FastQCFastQC Report
Thu 26 May 2016
SRR1511978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926525
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30960.16070385798263712No Hit
GTCCTACAGTGGACATTTCTAAATT29790.1546307470705026No Hit
CTGTAGGACGTGGAATATGGCAAGA27710.14383410544893008No Hit
GTCCTAAAGTGTGTATTTCTCATTT23300.12094314893396141No Hit
CTTTAGGACGTGAAATATGGCGAGG21660.11243041227079846No Hit
GTCCTACAGTGTGCATTTCTCATTT19490.10116660827136945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT350.002223338516.2271184
CGGTAGG558.409652E-616.0351241
CGTTAGA603.1497946E-413.0656571
GACCTAC1204.5292836E-1013.0656571
TAGGACC6050.012.3603264
CCAACGA1104.0088707E-812.04803219
ATTAGAC1251.9324943E-811.3589823
TTAGGAC9000.011.1486313
GGCGAGG6650.011.10334119
CCCGTTT1408.8366505E-910.81807912
TCCCGTT1502.2646418E-910.72792811
TACACCG803.8992654E-410.6490465
ATCCCGT1451.5268597E-810.44504310
AGGACGT15150.010.4342685
GACGTGA7550.010.2807577
GTTCTAA2300.010.2252961
GTCCTAC15900.010.1690251
GGACCTG10950.010.1141626
GGACGTG15050.010.0633296
TCCAACG1606.542905E-910.05795518
Produced by FastQC (version 0.11.5)