FastQCFastQC Report
Thu 26 May 2016
SRR1511978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926525
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37880.19662345414671492No Hit
GTCCTACAGTGGACATTTCTAAATT31910.16563501641556688No Hit
CTGTAGGACGTGGAATATGGCAAGA30490.1582642322316087No Hit
TATCAACGCAGAGTACTTTTTTTTT25160.13059783807632916No Hit
GTCCTAAAGTGTGTATTTCTCATTT24750.1284696539105384No Hit
CTTTAGGACGTGAAATATGGCGAGG23090.11985310338562957No Hit
GGTATCAACGCAGAGTACTTTTTTT22470.11663487367150698No Hit
GTCCTACAGTGTGCATTTCTCATTT20980.10890074097143822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT1207.4578566E-1012.66059412
GGCGAGG7150.012.35168119
TAGGACC5750.012.2310184
GCCGGTT1405.2750693E-1112.20842911
AAGGCGT1406.8030204E-1011.5352736
CGGTTTC1406.85759E-1011.53018413
TCCAACG1501.7826096E-1011.3948318
TGCCGGT1451.2278178E-911.1325910
CTAGACT2050.011.1264384
GTATTAA2500.011.07045651
GACGTGA8900.010.989117
ACGTGAA9750.010.9075898
GCGTGCG700.001497662310.8519379
GGACGTG15800.010.8223716
AGGACGT16100.010.8024915
TTAGGAC10050.010.6859013
CCAACGA803.7855827E-410.683207519
AACCGTG803.7883074E-410.6823767
TGGCGAG16050.010.64937318
GTCCTAC15400.010.5350291
Produced by FastQC (version 0.11.5)