FastQCFastQC Report
Thu 26 May 2016
SRR1511977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736388
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31970.1841178354146654No Hit
GGTATCAACGCAGAGTACTTTTTTT19570.11270522486909608No Hit
TATCAACGCAGAGTACTTTTTTTTT19490.11224449834944725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1800.012.09497453
TCGAACT1052.8502654E-711.71969119
CTAATAC1407.221388E-1011.4939813
TAGGACC3550.011.4654144
TCCAACG1105.2051837E-711.18697818
CCCGTTT951.4160816E-510.96020512
TAATACC1658.54925E-1110.8998194
CGTTAGG650.00844171510.5557791
CCTATAC1606.548362E-910.0572343
AGGACGT9850.09.8980725
ATCCCGT1151.0715219E-59.87718210
TTAATAC1451.7043931E-79.7920333
TAATACT2151.0913936E-119.6857624
GTAGGAC9400.09.6670293
AATACTG2350.09.6670295
GTATAAG2251.4551915E-119.5839781
GGCGAGG4500.09.46590419
TAGGACG10400.09.4656324
CCGTTTC900.00115117659.46563213
TTAGACC1107.112326E-59.46563154
Produced by FastQC (version 0.11.5)