FastQCFastQC Report
Thu 26 May 2016
SRR1511977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736388
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38120.21953618661266955No Hit
TATCAACGCAGAGTACTTTTTTTTT23400.134762506997284No Hit
GGTATCAACGCAGAGTACTTTTTTT22320.12854269898202475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC1501.6189006E-1011.4558171
ATAGGAC1701.0913936E-1111.1826263
TAGGACC3300.010.6573824
CTAATAC1409.609357E-810.1841773
GTCTTCG856.30417E-410.1080731
GTATAAT2600.09.9136881
TAGGACA3750.09.63196854
GGTATCA12100.09.6254111
AGCACCG800.00449543269.5052325
TAGGACT2700.09.5052324
GGGGCGT800.004509359.5016716
GTTCTAG2159.640644E-119.3245021
GTATTAG1659.880932E-89.2572261
GTCTTAT1553.967998E-79.2385621
CTCTTAG1451.5962632E-69.2173231
TTAGGAC6450.09.1368133
TTAAGAC1502.680672E-68.8715513
TAATACT2800.08.8262874
GTAAGAC1401.0772721E-58.8262873
TGGCGAG9550.08.74941518
Produced by FastQC (version 0.11.5)