##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511977_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1736388 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2639070299956 33.0 31.0 34.0 28.0 34.0 2 31.46362103400853 33.0 31.0 34.0 28.0 34.0 3 31.58211816713776 34.0 31.0 34.0 28.0 34.0 4 35.122913196820065 37.0 35.0 37.0 32.0 37.0 5 34.81075773387054 37.0 35.0 37.0 32.0 37.0 6 34.75742633558859 37.0 35.0 37.0 32.0 37.0 7 34.63274337302492 37.0 35.0 37.0 32.0 37.0 8 34.5573328081051 37.0 35.0 37.0 31.0 37.0 9 36.121736616470514 39.0 37.0 39.0 32.0 39.0 10 35.95579789770489 39.0 37.0 39.0 31.0 39.0 11 35.95078922452816 39.0 37.0 39.0 31.0 39.0 12 35.809404349719074 39.0 37.0 39.0 30.0 39.0 13 35.781798192569866 39.0 37.0 39.0 30.0 39.0 14 36.88851166905093 40.0 37.0 41.0 31.0 41.0 15 36.8175632404739 40.0 37.0 41.0 31.0 41.0 16 36.720621197566444 40.0 37.0 41.0 30.0 41.0 17 36.64120807100717 40.0 37.0 41.0 30.0 41.0 18 36.56928981310629 40.0 36.0 41.0 30.0 41.0 19 36.58754667735552 40.0 37.0 41.0 30.0 41.0 20 36.476177559393406 40.0 36.0 41.0 30.0 41.0 21 36.371095630700054 40.0 36.0 41.0 30.0 41.0 22 36.25260540846861 40.0 36.0 41.0 30.0 41.0 23 36.360478763962895 40.0 37.0 41.0 30.0 41.0 24 36.2754516847617 40.0 37.0 41.0 30.0 41.0 25 35.80039369081104 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16148648175847669 1101 2 0.20936792786275404 1101 3 0.28030790466076283 1101 4 0.22690291908199356 1101 5 0.3466668382671543 1101 6 0.3087798974310729 1101 7 0.19900770694597014 1101 8 0.21957346189312688 1101 9 0.2823013011197162 1101 10 0.13791930168712696 1101 11 0.22001550233464684 1101 12 0.33020005863850344 1101 13 0.11253073420935067 1101 14 0.037386924203801186 1101 15 0.15990016975442956 1101 16 0.4072148703599652 1101 17 0.32948272103454457 1101 18 0.2676501176369044 1101 19 0.20707159460000213 1101 20 0.22472926983589048 1101 21 0.2703761975009442 1101 22 0.29773617913375716 1101 23 0.03478559786707791 1101 24 -0.0014642558074200451 1101 25 0.08759937015765473 1102 1 0.33055594440592273 1102 2 0.3122541955196638 1102 3 0.2169964149272552 1102 4 0.2899406356976897 1102 5 0.38990551600124945 1102 6 0.45982110902274087 1102 7 0.343757961787972 1102 8 0.40711387255279874 1102 9 0.6046953596605604 1102 10 0.6685556785620363 1102 11 0.7196048426579296 1102 12 0.7118164850255724 1102 13 0.6853281712269705 1102 14 0.7872631438047861 1102 15 0.7057688169074865 1102 16 0.7645703385411196 1102 17 0.6058828957833384 1102 18 0.7745381327818066 1102 19 0.7114024523253519 1102 20 0.8251486669525363 1102 21 0.6141303841909149 1102 22 0.7200982003948582 1102 23 0.7486926897558206 1102 24 0.6796133617839644 1102 25 0.6858810069714067 1103 1 0.34327053558619625 1103 2 0.2859214908687129 1103 3 0.3729493323658666 1103 4 0.3704538927360588 1103 5 0.49695436221144007 1103 6 0.5388029031969523 1103 7 0.3434723656616967 1103 8 0.42521844153446864 1103 9 0.4718753575464092 1103 10 0.40862574233899096 1103 11 0.5538674761138438 1103 12 0.6865753510234995 1103 13 0.4987523018742195 1103 14 0.5242428349529789 1103 15 0.5241678837128703 1103 16 0.6912095953977442 1103 17 0.651613882777589 1103 18 0.5259114977903465 1103 19 0.7955190423076033 1103 20 0.6617491086992686 1103 21 0.7226383669735483 1103 22 0.7571619013126636 1103 23 0.797991773808242 1103 24 0.8495523295208756 1103 25 0.9058133911612458 1104 1 0.23765985954765512 1104 2 0.15104984060391402 1104 3 0.30471074299537904 1104 4 0.3461768069734745 1104 5 0.2864109321943715 1104 6 0.3367414830228199 1104 7 0.2837872800558898 1104 8 0.292587055122155 1104 9 0.4842060630244731 1104 10 0.4760071304751321 1104 11 0.33032412847809667 1104 12 0.3915457028207783 1104 13 0.4622842322572822 1104 14 0.5511368885821284 1104 15 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23 -1.1296816239992467 2314 24 -1.0485765057896188 2314 25 -1.26846867739021 2315 1 -0.7789038983243586 2315 2 -0.7636191128609511 2315 3 -0.9747315681201805 2315 4 -0.8150982555669515 2315 5 -0.9405845594888049 2315 6 -0.9384257347817737 2315 7 -0.9950229407127367 2315 8 -0.9713759442989556 2315 9 -1.1296014013356057 2315 10 -1.1332741874016037 2315 11 -1.1954157234954295 2315 12 -1.2318127099480236 2315 13 -1.1412651362131427 2315 14 -1.3619555774933403 2315 15 -1.3271526055443772 2315 16 -1.401196531362345 2315 17 -1.224108272306573 2315 18 -1.2060842744059883 2315 19 -1.2446666570036982 2315 20 -1.475903667625495 2315 21 -1.5805346130831026 2315 22 -1.6144028260758887 2315 23 -1.5363622782303423 2315 24 -1.64741713255318 2315 25 -1.6636710050435184 2316 1 -1.1943203013864334 2316 2 -1.2086839163761418 2316 3 -1.1793426606013107 2316 4 -1.1965663055383118 2316 5 -1.4257986179393072 2316 6 -1.5870976862162962 2316 7 -1.4505526447726496 2316 8 -1.4915330814436345 2316 9 -1.6304222343376367 2316 10 -1.7354528577281982 2316 11 -1.895791280810272 2316 12 -1.9448530417383338 2316 13 -1.7958853900341083 2316 14 -2.2422750604941584 2316 15 -2.119615646164263 2316 16 -1.9089253603126437 2316 17 -1.968948248475563 2316 18 -1.8779248509596442 2316 19 -1.9265312374201002 2316 20 -2.0074872426683967 2316 21 -2.0949361240703723 2316 22 -2.0076469429400134 2316 23 -2.3146034623133502 2316 24 -2.1783102076174004 2316 25 -2.036324348010666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21004.0 3 1.0 4 385.0 5 3277.0 6 4401.0 7 3609.0 8 2862.0 9 2571.0 10 2531.0 11 2343.0 12 2329.0 13 2261.0 14 2152.0 15 2274.0 16 2286.0 17 2371.0 18 2627.0 19 2850.0 20 3488.0 21 4488.0 22 5819.0 23 7461.0 24 8823.0 25 10404.0 26 12286.0 27 14122.0 28 16438.0 29 20081.0 30 25361.0 31 33556.0 32 43354.0 33 57568.0 34 77252.0 35 106286.0 36 163337.0 37 316703.0 38 747427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.436699042945094 19.822683607172962 14.192138853674921 24.548478496207014 2 14.851922496584866 21.132143276733082 40.15300727717538 23.862926949506676 3 18.019014183465906 25.454449120818616 30.744223065351754 25.782313630363717 4 12.73517209287325 18.06716010476921 38.52969497600767 30.66797282634987 5 13.456327873801449 37.789336081823265 35.66298197714982 13.091354067225467 6 30.865110200845514 37.74457465890489 18.596173004754213 12.794142135495385 7 27.432693614560797 32.116381822495896 22.750445176999612 17.700479385943694 8 25.1783011631041 36.217308573890165 21.05157372660949 17.552816536396243 9 26.25547976604307 15.80505048410839 20.58192063064246 37.35754911920608 10 14.938020764944241 29.164161466216076 34.28928327078971 21.608534498049973 11 33.4905562581635 23.086948308788127 24.862242770625 18.560252662423377 12 22.949248670228084 25.04480565403585 31.622770947507124 20.383174728228944 13 28.13610782843466 22.103354780152824 26.35862491562946 23.401912475783064 14 21.308831897018408 22.002801217239465 27.920315044794137 28.76805184094799 15 23.023310458261633 30.01840602445997 23.96572655420332 22.992556963075074 16 22.506490484845553 28.32489051986077 27.110357823251483 22.058261172042194 17 21.861300584892316 28.46690946954252 27.719380691412287 21.952409254152876 18 21.646256481846223 27.827478651084895 29.15598357049231 21.37028129657657 19 23.26818660345499 27.28681608027699 27.705558895822826 21.7394384204452 20 23.307073826284118 27.54708483765428 27.94549832497018 21.200343011091423 21 23.25666844046377 26.910978421873455 27.333234277131606 22.499118860531173 22 23.009200708597387 27.578398376399743 27.54223134460731 21.870169570395557 23 22.71767600328959 27.366521768176238 27.50088113946883 22.41492108906535 24 22.784972698209387 27.12411732081346 27.97113226552741 22.119777715449743 25 22.83258485146422 27.30694553160068 27.779764897784275 22.080704719150827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 362.0 1 362.0 2 384.5 3 407.0 4 407.0 5 407.0 6 1033.0 7 1659.0 8 1659.0 9 1659.0 10 2459.5 11 3260.0 12 3260.0 13 3260.0 14 5849.5 15 8439.0 16 8439.0 17 8439.0 18 15396.5 19 22354.0 20 22354.0 21 22354.0 22 34908.0 23 47462.0 24 47462.0 25 47462.0 26 66109.0 27 84756.0 28 84756.0 29 84756.0 30 107354.5 31 129953.0 32 129953.0 33 129953.0 34 147194.5 35 164436.0 36 164436.0 37 164436.0 38 179615.5 39 194795.0 40 194795.0 41 194795.0 42 207831.5 43 220868.0 44 220868.0 45 220868.0 46 222446.5 47 224025.0 48 224025.0 49 224025.0 50 217095.5 51 210166.0 52 210166.0 53 210166.0 54 188583.5 55 167001.0 56 167001.0 57 167001.0 58 142602.5 59 118204.0 60 118204.0 61 118204.0 62 95786.5 63 73369.0 64 73369.0 65 73369.0 66 55900.5 67 38432.0 68 38432.0 69 38432.0 70 27636.0 71 16840.0 72 16840.0 73 16840.0 74 11662.5 75 6485.0 76 6485.0 77 6485.0 78 4366.5 79 2248.0 80 2248.0 81 2248.0 82 1456.0 83 664.0 84 664.0 85 664.0 86 415.0 87 166.0 88 166.0 89 166.0 90 99.0 91 32.0 92 32.0 93 32.0 94 18.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46446992262098097 2 0.0 3 0.0 4 0.0 5 0.03726125727659947 6 0.050391963086591246 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 5.7590814956104285E-5 21 0.0 22 0.0 23 0.0 24 0.0031674948225857355 25 0.011921298695913586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1736388.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.38820032332004 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11000679651022 59.437956205349494 2 12.12885413716973 17.559718459475427 3 3.1864044092649446 6.919742420669429 4 1.1351513852271342 3.28686263484464 5 0.502614738895187 1.8191688202299 6 0.2561635556274659 1.112593126817696 7 0.1556460357390857 0.7886855490228096 8 0.10777407836920036 0.6241257259720694 9 0.07433659955681315 0.4842983394065711 >10 0.3124771807608038 3.8437591615338094 >50 0.017220130476451483 0.849628006491201 >100 0.011285212535975805 1.6301848246628912 >500 0.001350676066980432 0.6747487940588505 >1k 7.150638001661106E-4 0.9685279314653118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3812 0.21953618661266955 No Hit TATCAACGCAGAGTACTTTTTTTTT 2340 0.134762506997284 No Hit GGTATCAACGCAGAGTACTTTTTTT 2232 0.12854269898202475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 5.759081495610428E-5 6 0.0 0.0 0.0 0.0 1.1518162991220856E-4 7 0.0 5.759081495610428E-5 0.0 0.0 1.1518162991220856E-4 8 0.0 5.759081495610428E-5 0.0 0.0 1.1518162991220856E-4 9 0.0 5.759081495610428E-5 0.0 5.759081495610428E-5 1.7277244486831283E-4 10 0.0 5.759081495610428E-5 0.0 5.759081495610428E-5 1.7277244486831283E-4 11 0.0 5.759081495610428E-5 0.0 5.759081495610428E-5 1.7277244486831283E-4 12 0.0 5.759081495610428E-5 0.0 5.759081495610428E-5 2.303632598244171E-4 13 0.0 5.759081495610428E-5 0.0 5.759081495610428E-5 3.4554488973662567E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAC 150 1.6189006E-10 11.455817 1 ATAGGAC 170 1.0913936E-11 11.182626 3 TAGGACC 330 0.0 10.657382 4 CTAATAC 140 9.609357E-8 10.184177 3 GTCTTCG 85 6.30417E-4 10.108073 1 GTATAAT 260 0.0 9.913688 1 TAGGACA 375 0.0 9.6319685 4 GGTATCA 1210 0.0 9.625411 1 AGCACCG 80 0.0044954326 9.505232 5 TAGGACT 270 0.0 9.505232 4 GGGGCGT 80 0.00450935 9.501671 6 GTTCTAG 215 9.640644E-11 9.324502 1 GTATTAG 165 9.880932E-8 9.257226 1 GTCTTAT 155 3.967998E-7 9.238562 1 CTCTTAG 145 1.5962632E-6 9.217323 1 TTAGGAC 645 0.0 9.136813 3 TTAAGAC 150 2.680672E-6 8.871551 3 TAATACT 280 0.0 8.826287 4 GTAAGAC 140 1.0772721E-5 8.826287 3 TGGCGAG 955 0.0 8.749415 18 >>END_MODULE