FastQCFastQC Report
Thu 26 May 2016
SRR1511976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401443
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50460.360057455065957No Hit
GGTATCAACGCAGAGTACTTTTTTT32950.23511480666712808No Hit
TATCAACGCAGAGTACTTTTTTTTT32130.22926369463474433No Hit
CTTTAGGACGTGAAATATGGCGAGG23800.1698249589886995No Hit
GTCCTACAGTGGACATTTCTAAATT23560.16811243839385548No Hit
GTCCTAAAGTGTGTATTTCTCATTT22300.1591217052709243No Hit
CTGTAGGACGTGGAATATGGCAAGA22110.15776595980000613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA953.2196112E-1014.944509519
CGTTAGG551.4927126E-414.2685091
ACGGTAC400.00541014114.1972863
TCCAACG1650.013.76706418
CTCAACG603.120811E-413.0794662
CGGTTTC1251.4952093E-912.11501613
TAGGACA4950.011.8549724
CTAAACT1201.0571966E-811.831074
TACGCTG658.275854E-411.6490555
GTATTAG1352.8558134E-911.6261921
TCTAGAC1002.0168645E-611.3578283
TAGGACC3750.011.3578274
GGTATCA10750.011.2240071
CGGTAGG700.00114079211.2109721
GGCGAGG8500.010.80107219
TGCCGGT1356.000482E-810.51650810
TTAGGAC11300.010.3862153
GACGTGA9200.010.2878887
GGACGTG15850.010.1515826
GCCGGTT1502.5867848E-810.09584611
Produced by FastQC (version 0.11.5)