FastQCFastQC Report
Thu 26 May 2016
SRR1511976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401443
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64080.4572429988233556No Hit
GGTATCAACGCAGAGTACTTTTTTT42010.29976245912249017No Hit
TATCAACGCAGAGTACTTTTTTTTT40980.29241289156961786No Hit
CTTTAGGACGTGAAATATGGCGAGG27990.19972271437368483No Hit
GTCCTACAGTGGACATTTCTAAATT26440.18866268553198381No Hit
CTGTAGGACGTGGAATATGGCAAGA25150.17945788733469717No Hit
GTCCTAAAGTGTGTATTTCTCATTT25070.17888704713641582No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18390.1312218905799237No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15410.10995809319394366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG500.001495431613.3047015
GGTATCA13950.012.5226651
TCCAACG2050.012.04314718
CCAACGA1353.7289283E-1011.95907619
TAGGACC4450.011.5321644
GGCGAGG7050.011.04605419
CCAACGT803.7815137E-410.68402819
GGACGTG14200.010.5031076
TAGACTG1553.6379788E-910.4230375
AGGACGT14200.010.30645755
CCACCTT5050.010.15330313
TCGAACT856.611805E-410.05555419
CACCTTT5500.010.01349714
GACGTGA8350.010.0069377
CTCGAAC951.6513353E-49.99532418
GACGTGG6950.09.8367747
GGACCTG9050.09.7620466
AGGACCT9450.09.7547675
TGGCGAG15450.09.58774718
ATAGTCC1002.7446437E-49.5033583
Produced by FastQC (version 0.11.5)