FastQCFastQC Report
Thu 26 May 2016
SRR1511975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671197
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28360.1696987249259064No Hit
GTCCTACAGTGGACATTTCTAAATT21270.1272740436944298No Hit
GTCCTAAAGTGTGTATTTCTCATTT19130.11446885076983743No Hit
CTGTAGGACGTGGAATATGGCAAGA18760.11225486881558547No Hit
CTTTAGGACGTGAAATATGGCGAGG17040.10196284459581964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG601.068247E-616.322041
CTGTCCG604.2159998E-412.6220269
TCCAACG1700.012.25115818
GAACCGT550.003150815612.0482996
GCCATAA1251.1683369E-811.7518691
ACCGTCC1052.8476097E-711.7204538
TAGGACC4950.011.66581254
ACTGTTC4250.011.3598248
CACCGTC600.006032411.0442727
ACGAAAT700.001535691710.81920412
ACCGTGC700.001536074910.818888
CCAACGA1159.222549E-710.70160419
TAGGACT2400.010.6498354
GTCCTAA7250.010.53615951
AATCCCG1909.094947E-1210.46330819
GATCTAG750.002056897910.4461061
CCGTGCA1002.4939449E-510.4131729
CCCTATA854.9839925E-410.3692962
GGCGAGG5350.010.26307719
TCTACAC2900.010.1193843
Produced by FastQC (version 0.11.5)