##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511975_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1671197 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.813369100112077 31.0 30.0 34.0 25.0 34.0 2 26.88571724338902 31.0 25.0 34.0 10.0 34.0 3 28.85497999338199 31.0 28.0 34.0 16.0 34.0 4 33.370327974499716 35.0 33.0 37.0 28.0 37.0 5 33.83033837423117 37.0 35.0 37.0 30.0 37.0 6 34.115200661561744 37.0 35.0 37.0 30.0 37.0 7 34.18769181610546 37.0 35.0 37.0 30.0 37.0 8 34.224153107024485 37.0 35.0 37.0 30.0 37.0 9 35.882949167572704 39.0 37.0 39.0 31.0 39.0 10 35.80160747057349 39.0 37.0 39.0 30.0 39.0 11 35.8128580891421 39.0 37.0 39.0 30.0 39.0 12 35.72916598103036 39.0 37.0 39.0 30.0 39.0 13 35.72731940040582 39.0 37.0 39.0 30.0 39.0 14 36.976160799714215 40.0 38.0 41.0 31.0 41.0 15 36.92563773151819 40.0 38.0 41.0 31.0 41.0 16 36.875648412485184 40.0 38.0 41.0 31.0 41.0 17 36.8505065530874 40.0 37.0 41.0 31.0 41.0 18 36.80203829949431 40.0 37.0 41.0 31.0 41.0 19 36.82839425872593 40.0 38.0 41.0 30.0 41.0 20 36.738256471259824 40.0 37.0 41.0 30.0 41.0 21 36.685543356049585 40.0 37.0 41.0 30.0 41.0 22 36.595170407797525 40.0 37.0 41.0 30.0 41.0 23 36.511047470764964 40.0 37.0 41.0 30.0 41.0 24 36.426868286623304 40.0 37.0 41.0 30.0 41.0 25 36.33920956057245 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.036278669086343 1101 2 4.105796484440361 1101 3 2.227933351138134 1101 4 1.2526887462471024 1101 5 0.9019270847105574 1101 6 0.5409418621384177 1101 7 0.23658862117240176 1101 8 0.08937261744834046 1101 9 0.21368480002249868 1101 10 0.279470406557742 1101 11 0.586471440964111 1101 12 0.23045354937349316 1101 13 0.3178704130172463 1101 14 0.23465201870729402 1101 15 0.4631677722803005 1101 16 0.42804552637015547 1101 17 0.34277779800201813 1101 18 0.4886134653102445 1101 19 0.391066570847741 1101 20 0.4912159903101738 1101 21 0.2766424256588351 1101 22 0.29311693875605727 1101 23 0.15755286976136063 1101 24 0.19864519507727607 1101 25 0.13227050587548206 1102 1 1.7100874707847495 1102 2 4.405326390102243 1102 3 2.5500421512402944 1102 4 1.6953124951895902 1102 5 1.3025536111204374 1102 6 1.046538299318172 1102 7 0.8212550856479552 1102 8 0.7280185222801734 1102 9 0.7819370706819129 1102 10 0.6359383938516245 1102 11 0.7570127278573366 1102 12 0.8168894521302121 1102 13 0.9682359532665785 1102 14 0.9355028176282332 1102 15 0.8852470418382978 1102 16 0.9273782956966983 1102 17 0.9730394226608183 1102 18 1.0541960151530105 1102 19 0.8856880211965219 1102 20 0.9687991148013708 1102 21 0.8271197031022837 1102 22 0.8955647814300818 1102 23 0.9272712920321737 1102 24 0.9055250137437696 1102 25 0.9910897629827673 1103 1 1.6188708333422959 1103 2 4.441317943225869 1103 3 2.6757391526938754 1103 4 1.8033794830858696 1103 5 1.4758826620936745 1103 6 1.2828866982468483 1103 7 1.0794190579525562 1103 8 0.9934259435997035 1103 9 1.162210647204894 1103 10 0.9415947391887229 1103 11 1.169972969773859 1103 12 1.0460117629632677 1103 13 1.137920000317827 1103 14 1.236934935644605 1103 15 1.294364996658878 1103 16 1.238324197493867 1103 17 1.20184213475207 1103 18 1.1525945492601153 1103 19 1.1597830043643214 1103 20 1.3668984097902168 1103 21 1.3405791503621813 1103 22 1.3027832938518245 1103 23 1.421833378625287 1103 24 1.4404062571643763 1103 25 1.52918040907727 1104 1 1.3356931393745626 1104 2 3.8611656378106254 1104 3 2.163464603786501 1104 4 1.459635362886928 1104 5 0.9802189558632008 1104 6 0.7087431210273039 1104 7 0.5484879716966873 1104 8 0.5987276102447865 1104 9 0.6032800911309266 1104 10 0.6776818444184016 1104 11 0.3950679416536502 1104 12 0.5408108765844446 1104 13 0.5604372674129579 1104 14 0.6264769453234678 1104 15 0.5531775567899615 1104 16 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-1.046457607311524 2315 15 -1.3745618710354748 2315 16 -1.3099199845452745 2315 17 -1.212827988616425 2315 18 -1.266312236395862 2315 19 -1.214928397310132 2315 20 -1.2717855559329792 2315 21 -1.2247334686724827 2315 22 -1.5809546601641955 2315 23 -1.37296446650722 2315 24 -1.229476774679263 2315 25 -1.1388868178134857 2316 1 -0.5559274058359875 2316 2 -0.13952664699466766 2316 3 -0.055627642006395916 2316 4 -0.3093044753103982 2316 5 -0.6725895661226247 2316 6 -0.6900104576808204 2316 7 -0.6912209662155817 2316 8 -0.8536781951944832 2316 9 -0.959806097864373 2316 10 -0.9882421258581076 2316 11 -1.044227971057353 2316 12 -0.9324742756194482 2316 13 -1.1329358108254084 2316 14 -1.2419071514470232 2316 15 -1.0559543680642705 2316 16 -1.3237145578468628 2316 17 -1.3548882610012427 2316 18 -1.3910807344997522 2316 19 -1.1185606148148253 2316 20 -1.194115357418795 2316 21 -1.1966225241447503 2316 22 -1.2299515902705735 2316 23 -1.3526584430483553 2316 24 -1.3485432918408478 2316 25 -1.3562151284480848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47317.0 3 2.0 4 429.0 5 1915.0 6 2358.0 7 2134.0 8 1924.0 9 1885.0 10 1808.0 11 1698.0 12 1795.0 13 1726.0 14 1740.0 15 1871.0 16 1900.0 17 2109.0 18 2355.0 19 2976.0 20 3993.0 21 4927.0 22 6096.0 23 7339.0 24 8612.0 25 9880.0 26 11429.0 27 12749.0 28 15246.0 29 18363.0 30 23216.0 31 30212.0 32 40121.0 33 53797.0 34 75877.0 35 113838.0 36 188974.0 37 264735.0 38 703851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47751761162807 19.14513968131824 13.295619846134239 25.08172286091945 2 16.766649065802678 21.773176654692712 39.331555088950395 22.128619190554215 3 18.27558330944826 25.150236626801032 30.179685578660088 26.394494485090625 4 12.172771971227808 17.415600913596663 39.02747551605227 31.38415159912326 5 13.017555680150217 37.97876611793822 36.06600538416476 12.937672817746801 6 30.785119887122825 37.698128945899256 18.86486153337997 12.651889633597953 7 27.358294683391605 31.989167046135197 22.889402027409098 17.763136243064103 8 24.59584357798632 37.177723511949814 21.27086154415069 16.955571365913176 9 26.24340517605046 15.843135189926741 20.704142001212304 37.209317632810496 10 14.635497789907474 29.315514568300443 34.814267857110806 21.234719784681282 11 33.548648064830175 22.923210130224025 25.195353988787677 18.33278781615812 12 22.996451046764683 24.839501267654263 32.20517988005005 19.958867805531007 13 28.478210528142405 22.298268845623824 26.250406146013905 22.973114480219866 14 20.82028629778536 22.37803203332701 28.208942452625273 28.59273921626236 15 22.68864771777355 30.895998496885763 23.861220430625472 22.554133354715216 16 21.82962271952379 28.48012532334608 28.0802921498782 21.609959807251926 17 21.391852666083054 28.77255045335768 28.22569691065745 21.609899969901814 18 21.213626218443146 28.004715202939224 29.97283372925879 20.80882484935884 19 23.087983886844093 27.346409515187165 27.682279776996026 21.883326820972712 20 23.04168780936151 27.874848011948416 28.402079005064675 20.681385173625404 21 22.81519556950474 27.163770947464165 27.328268941354576 22.692764541676517 22 22.64448666357503 28.218110012416414 27.73958442413272 21.397818899875837 23 22.330296353829453 27.83437790588435 27.776275178347248 22.059050561938946 24 22.574956752555202 27.211154639459025 28.561085258051566 21.652803349934207 25 22.70507292425858 27.574309502353415 28.082511461919857 21.63810611146814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 354.0 1 354.0 2 407.5 3 461.0 4 461.0 5 461.0 6 1007.0 7 1553.0 8 1553.0 9 1553.0 10 2416.5 11 3280.0 12 3280.0 13 3280.0 14 5694.5 15 8109.0 16 8109.0 17 8109.0 18 14763.0 19 21417.0 20 21417.0 21 21417.0 22 33699.5 23 45982.0 24 45982.0 25 45982.0 26 65237.0 27 84492.0 28 84492.0 29 84492.0 30 109155.5 31 133819.0 32 133819.0 33 133819.0 34 150552.5 35 167286.0 36 167286.0 37 167286.0 38 180333.0 39 193380.0 40 193380.0 41 193380.0 42 203497.5 43 213615.0 44 213615.0 45 213615.0 46 213829.5 47 214044.0 48 214044.0 49 214044.0 50 205521.5 51 196999.0 52 196999.0 53 196999.0 54 175079.5 55 153160.0 56 153160.0 57 153160.0 58 130529.0 59 107898.0 60 107898.0 61 107898.0 62 86971.0 63 66044.0 64 66044.0 65 66044.0 66 50665.0 67 35286.0 68 35286.0 69 35286.0 70 25286.5 71 15287.0 72 15287.0 73 15287.0 74 10508.5 75 5730.0 76 5730.0 77 5730.0 78 3886.5 79 2043.0 80 2043.0 81 2043.0 82 1354.0 83 665.0 84 665.0 85 665.0 86 431.5 87 198.0 88 198.0 89 198.0 90 134.0 91 70.0 92 70.0 93 70.0 94 43.5 95 17.0 96 17.0 97 17.0 98 12.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.335692919506198 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.1886145080442344E-4 19 9.573976018386821E-4 20 7.778855514939292E-4 21 0.0031115422059757166 22 0.0013164217025281879 23 0.0011369096521834348 24 0.0 25 6.582108512640939E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1671197.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.92902114479988 #Duplication Level Percentage of deduplicated Percentage of total 1 86.03009678291701 66.18211134502202 2 9.95338458488868 15.314082663864525 3 2.2813400216649526 5.2650376429542405 4 0.7479397733813764 2.301530985659709 5 0.32980686286072475 1.2685859563356403 6 0.1633717813331687 0.7540818732385773 7 0.11725007913265362 0.6313953671777757 8 0.07179576352306088 0.44185422481380815 9 0.05159405431860338 0.35721720860596134 >10 0.22453279478893617 3.0313920015363 >50 0.014890915459754179 0.801417429666478 >100 0.011637976649317712 1.784400675803264 >500 0.0015724060545753487 0.8295082889910275 >1k 7.862030272876744E-4 1.0373843363305943 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2836 0.1696987249259064 No Hit GTCCTACAGTGGACATTTCTAAATT 2127 0.1272740436944298 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1913 0.11446885076983743 No Hit CTGTAGGACGTGGAATATGGCAAGA 1876 0.11225486881558547 No Hit CTTTAGGACGTGAAATATGGCGAGG 1704 0.10196284459581964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.983735011491763E-5 0.0 9 0.0 0.0 0.0 5.983735011491763E-5 0.0 10 0.0 0.0 0.0 5.983735011491763E-5 0.0 11 0.0 0.0 0.0 5.983735011491763E-5 0.0 12 0.0 0.0 0.0 5.983735011491763E-5 0.0 13 0.0 0.0 0.0 5.983735011491763E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 60 1.068247E-6 16.32204 1 CTGTCCG 60 4.2159998E-4 12.622026 9 TCCAACG 170 0.0 12.251158 18 GAACCGT 55 0.0031508156 12.048299 6 GCCATAA 125 1.1683369E-8 11.751869 1 ACCGTCC 105 2.8476097E-7 11.720453 8 TAGGACC 495 0.0 11.6658125 4 ACTGTTC 425 0.0 11.359824 8 CACCGTC 60 0.0060324 11.044272 7 ACGAAAT 70 0.0015356917 10.819204 12 ACCGTGC 70 0.0015360749 10.81888 8 CCAACGA 115 9.222549E-7 10.701604 19 TAGGACT 240 0.0 10.649835 4 GTCCTAA 725 0.0 10.5361595 1 AATCCCG 190 9.094947E-12 10.463308 19 GATCTAG 75 0.0020568979 10.446106 1 CCGTGCA 100 2.4939449E-5 10.413172 9 CCCTATA 85 4.9839925E-4 10.369296 2 GGCGAGG 535 0.0 10.263077 19 TCTACAC 290 0.0 10.119384 3 >>END_MODULE