FastQCFastQC Report
Thu 26 May 2016
SRR1511975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671197
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33580.20093382168589338No Hit
GTCCTACAGTGGACATTTCTAAATT23560.14097679687074594No Hit
GTCCTAAAGTGTGTATTTCTCATTT21060.12601745934201655No Hit
TATCAACGCAGAGTACTTTTTTTTT20300.12146982073328279No Hit
CTGTAGGACGTGGAATATGGCAAGA20180.12075177253190379No Hit
CTTTAGGACGTGAAATATGGCGAGG19570.1171016941748938No Hit
GGTATCAACGCAGAGTACTTTTTTT19300.11548608572179103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1600.012.46193718
ACAGCGC550.003081199512.0843028
GTCCTAT2550.011.2345651
GTCCTAA7550.010.877441
TCTAGGC909.4726594E-510.5602143
AGGACGT12850.010.28085
TGTAGGA16050.010.2443932
GTCCTAC13400.010.1907561
TAGGACC5700.010.1711544
GGACGTG12650.010.1391716
GTATTAT1701.4242687E-910.1111091
TAGGACT2350.010.1108434
TAGGACA5750.010.0827094
CTGTAGG15250.010.0816411
ATAGGAC2002.5465852E-119.9794033
GTAGGAC15200.09.9418863
CTAGTAC1151.02091835E-59.91741853
CCAACGA1151.0318738E-59.90851619
CCTAGAC1752.5520421E-99.7757423
CTAGACT1951.6189006E-109.7478914
Produced by FastQC (version 0.11.5)