FastQCFastQC Report
Thu 26 May 2016
SRR1511974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505442
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30050.19960915133229976No Hit
TATCAACGCAGAGTACTTTTTTTTT18400.12222324074922847No Hit
GGTATCAACGCAGAGTACTTTTTTT18040.11983191647370008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGA550.00243695212.46822551
GTCCTAT1301.6116246E-912.0571851
GGTATCA9850.011.5369511
GTCTTAG1900.011.3432731
TAGGACT1502.2610038E-910.7283654
CTTATAC1252.3517896E-710.602153
GGACTAA650.0084749410.5500371
CTAACCT1002.4944082E-510.4128264
ATAGGAC1304.031972E-710.1943743
GTATTGG1251.7333768E-610.1883221
GTATAGG1002.0222634E-49.7964641
GTATTAA1406.954524E-79.7964621
CCAGTAC1554.2902684E-89.7715663
TCTATAC1554.2902684E-89.7715663
CTATACT1651.0824806E-89.753064
TAGCACC1107.105927E-59.4662064
AGGATAG1201.772933E-59.4662065
GGGGGCG1201.772933E-59.4662067
TTAGACC800.0046499879.4662054
ATAGCCC1502.786819E-79.4662053
Produced by FastQC (version 0.11.5)