##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511974_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1505442 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.974707760245828 31.0 30.0 34.0 25.0 34.0 2 27.007433697213177 31.0 26.0 34.0 10.0 34.0 3 28.995794590558788 31.0 28.0 34.0 16.0 34.0 4 33.55628114533805 35.0 33.0 37.0 28.0 37.0 5 34.01137871801106 37.0 35.0 37.0 30.0 37.0 6 34.30324383137975 37.0 35.0 37.0 31.0 37.0 7 34.38841748802013 37.0 35.0 37.0 31.0 37.0 8 34.43583877691734 37.0 35.0 37.0 32.0 37.0 9 36.11572614554397 39.0 37.0 39.0 32.0 39.0 10 36.03907955271608 39.0 37.0 39.0 32.0 39.0 11 36.052267041838874 39.0 37.0 39.0 32.0 39.0 12 35.95693158554099 39.0 37.0 39.0 32.0 39.0 13 35.95206258361332 39.0 37.0 39.0 32.0 39.0 14 37.22664174375366 40.0 38.0 41.0 32.0 41.0 15 37.166697222476856 40.0 38.0 41.0 31.0 41.0 16 37.11523061001354 40.0 38.0 41.0 31.0 41.0 17 37.08529587988112 40.0 38.0 41.0 31.0 41.0 18 37.03949139189687 40.0 38.0 41.0 31.0 41.0 19 37.065910211087505 40.0 38.0 41.0 31.0 41.0 20 36.976523173925 40.0 38.0 41.0 31.0 41.0 21 36.92601176265841 40.0 38.0 41.0 31.0 41.0 22 36.83156840316664 40.0 38.0 41.0 31.0 41.0 23 36.74701649083791 40.0 37.0 41.0 31.0 41.0 24 36.66784705089934 40.0 37.0 41.0 31.0 41.0 25 36.570043880800455 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0556040911127234 1101 2 3.8793127899044926 1101 3 2.0687294300607846 1101 4 1.1330379884237232 1101 5 0.7013536582661501 1101 6 0.3662089412494254 1101 7 -0.039030820558522805 1101 8 -0.10877043037405087 1101 9 -0.0787497291358008 1101 10 0.013471975606989872 1101 11 0.11039156449746912 1101 12 0.03902073757559066 1101 13 0.12907915358058375 1101 14 0.17040528299465763 1101 15 0.1319787741667895 1101 16 0.14157281369253383 1101 17 0.18964877756743448 1101 18 0.10337859208890876 1101 19 0.03505659466024724 1101 20 0.030674809177384077 1101 21 0.05350941101358586 1101 22 0.20440574761654062 1101 23 0.09296953719105971 1101 24 0.08104671678456299 1101 25 -0.05864758279044935 1102 1 1.3922579309423604 1102 2 4.163514123788598 1102 3 2.478704785689686 1102 4 1.5333501036687451 1102 5 1.0708222101236018 1102 6 0.8159566823491744 1102 7 0.6342327438717703 1102 8 0.6629036824085333 1102 9 0.6372364267032538 1102 10 0.3805643208126668 1102 11 0.5084422729479385 1102 12 0.637909033805343 1102 13 0.6451045118162924 1102 14 0.7104147878212643 1102 15 0.6640355063512544 1102 16 0.7114227617568787 1102 17 0.6629149488067085 1102 18 0.694127628572204 1102 19 0.6684926295834686 1102 20 0.8501357411316519 1102 21 0.7455540315624631 1102 22 0.6483583518187217 1102 23 0.7069076435248007 1102 24 0.7378923977031207 1102 25 0.6735103136350133 1103 1 1.2337305971006813 1103 2 3.9542892440014796 1103 3 2.415091163119232 1103 4 1.461936260124446 1103 5 1.0795658493449025 1103 6 0.9840874303923144 1103 7 0.7810869789443657 1103 8 0.7517503390120766 1103 9 0.6998832887190929 1103 10 0.5019384824348023 1103 11 0.600916713121606 1103 12 0.5798061409154087 1103 13 0.6142012423371384 1103 14 0.6576495025004618 1103 15 0.8316060390227022 1103 16 0.728550179630858 1103 17 0.729035924203913 1103 18 0.7296546200600389 1103 19 0.7346933578634491 1103 20 0.9919655445894264 1103 21 0.9470409365281469 1103 22 0.9012100036759918 1103 23 1.0614870151498934 1103 24 0.9881413823127616 1103 25 0.9186103743250129 1104 1 1.3383287635065777 1104 2 3.853704060388498 1104 3 2.231940243786479 1104 4 1.4874862726151292 1104 5 1.0332883534161112 1104 6 0.9257381158770173 1104 7 0.6730083271112406 1104 8 0.851503436794367 1104 9 0.8504875370525795 1104 10 0.7462012224577563 1104 11 0.7942578664338882 1104 12 0.8576960426240987 1104 13 0.9254093257786309 1104 14 0.8796126240056594 1104 15 0.8049990145653894 1104 16 0.8170100131761231 1104 17 0.8423545567281394 1104 18 0.894372866030622 1104 19 1.0694158487865764 1104 20 0.9003797542863268 1104 21 0.783331336361357 1104 22 0.9927352188819327 1104 23 1.0229856712279712 1104 24 1.0852010013564453 1104 25 0.960622256435542 1105 1 1.3877815429063531 1105 2 4.220012843215859 1105 3 2.4597788941564467 1105 4 1.3535550701941261 1105 5 1.0368664385598052 1105 6 0.8598818250094737 1105 7 0.5432409365417996 1105 8 0.576949917824237 1105 9 0.577191793875663 1105 10 0.32185080964697477 1105 11 0.5432274212996688 1105 12 0.5658337570967902 1105 13 0.5059246025796966 1105 14 0.4761223978681457 1105 15 0.4290953412996572 1105 16 0.5350987543879668 1105 17 0.6449831861095703 1105 18 0.6339744091674078 1105 19 0.683213599523711 1105 20 0.5773568982901267 1105 21 0.34543870512874264 1105 22 0.5775973471307765 1105 23 0.47878409446613546 1105 24 0.6369293470942878 1105 25 0.6651023426313714 1106 1 0.5834834919339897 1106 2 2.618749548082249 1106 3 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-0.3032839995697003 2315 8 -0.3043044916579163 2315 9 -0.3628909868025758 2315 10 -0.3683338790631865 2315 11 -0.4812454006121243 2315 12 -0.28915816865145416 2315 13 -0.30413999143438275 2315 14 -0.618287554627031 2315 15 -0.799780691499052 2315 16 -0.8035769115418248 2315 17 -0.6487776906130733 2315 18 -0.5499280413288048 2315 19 -0.5479020582534417 2315 20 -0.5069414257147002 2315 21 -0.560997962948953 2315 22 -0.7688343039361243 2315 23 -0.5093318611960314 2315 24 -0.4545934724657812 2315 25 -0.3955094191233286 2316 1 -0.2419885619914801 2316 2 0.21388938149373615 2316 3 0.26568669267520306 2316 4 -0.023893768314401598 2316 5 -0.3172776656576488 2316 6 -0.4613096595710786 2316 7 -0.6750735821796994 2316 8 -0.7735098099594921 2316 9 -0.6516437184570947 2316 10 -0.8493712610318838 2316 11 -0.9541766616323528 2316 12 -0.6898886522138667 2316 13 -0.756503340630033 2316 14 -0.9255706050937107 2316 15 -0.8149302261817013 2316 16 -0.9233033716579726 2316 17 -1.032716583246284 2316 18 -1.0086090861994208 2316 19 -0.8780186769246825 2316 20 -0.8607296603680794 2316 21 -0.8769916724847278 2316 22 -0.9041500866019447 2316 23 -0.9068395030208123 2316 24 -0.9759817316274635 2316 25 -0.8236354190280153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38170.0 3 1.0 4 353.0 5 1477.0 6 1869.0 7 1659.0 8 1596.0 9 1556.0 10 1453.0 11 1426.0 12 1463.0 13 1402.0 14 1462.0 15 1440.0 16 1586.0 17 1627.0 18 1934.0 19 2434.0 20 3072.0 21 3964.0 22 4892.0 23 5771.0 24 6779.0 25 7954.0 26 9044.0 27 10418.0 28 12438.0 29 15609.0 30 19932.0 31 26127.0 32 34892.0 33 49165.0 34 70133.0 35 105595.0 36 176596.0 37 248627.0 38 631526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.97694630547042 18.60782414732683 13.201239237380118 25.21399030982263 2 17.405925620402833 21.40028871932357 37.946861895923554 23.24692376435004 3 18.467931677208423 24.405523427671074 29.884645173975482 27.241899721145018 4 12.857818501144514 16.766238752472695 37.59965511789893 32.77628762848386 5 13.834209487977617 37.322128650589 35.170800336379614 13.672861525053772 6 32.11306993304283 36.36770645127006 18.049341056435328 13.469882559251781 7 28.088362089007745 31.626259928977667 22.060298570120935 18.22507941189365 8 25.820257439343397 35.67410767070402 20.48421659552477 18.021418294427814 9 26.49487658773968 15.61534751920034 20.070517495858358 37.81925839720162 10 15.597346161459559 28.433310615752717 33.6368986649768 22.33244455781093 11 34.24416217961237 22.550055066884013 24.213353951862643 18.99242880164098 12 23.434247217760632 24.33856634795628 30.963929530330624 21.26325690395246 13 28.63756956428743 21.609533944183834 25.831948358023755 23.920948133504975 14 21.82256108172882 21.297665403250342 27.131566676099112 29.748206838921725 15 23.398443779302024 29.311856584312117 23.639635402758792 23.65006423362707 16 23.095210576030162 27.549517018922014 26.484846310917327 22.870426094130494 17 22.330385361907002 27.78818446675461 27.25080076150393 22.630629409834455 18 22.341995730134965 27.102350551402456 28.444488433235865 22.11116528522672 19 23.759558718029112 26.772647319839088 27.183094287885474 22.28469967424633 20 23.836116362457606 26.80788397232957 27.410102335937676 21.945897329275148 21 23.63882268225866 26.364033724196023 26.898381015392708 23.09876257815261 22 23.69177096251992 26.904643443360726 26.876345560985587 22.527240033133765 23 23.197936532292832 26.64300560571851 27.138014662949452 23.02104319903921 24 23.397248117164263 26.365877928209784 27.443235940009647 22.793638014616306 25 23.34373563539068 26.59129979620522 27.317866717681788 22.747097850722316 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 572.0 1 572.0 2 546.5 3 521.0 4 521.0 5 521.0 6 1022.0 7 1523.0 8 1523.0 9 1523.0 10 2298.0 11 3073.0 12 3073.0 13 3073.0 14 4986.5 15 6900.0 16 6900.0 17 6900.0 18 11740.5 19 16581.0 20 16581.0 21 16581.0 22 25177.0 23 33773.0 24 33773.0 25 33773.0 26 47586.5 27 61400.0 28 61400.0 29 61400.0 30 79179.5 31 96959.0 32 96959.0 33 96959.0 34 112031.5 35 127104.0 36 127104.0 37 127104.0 38 141478.0 39 155852.0 40 155852.0 41 155852.0 42 169557.5 43 183263.0 44 183263.0 45 183263.0 46 190213.5 47 197164.0 48 197164.0 49 197164.0 50 195141.0 51 193118.0 52 193118.0 53 193118.0 54 176990.5 55 160863.0 56 160863.0 57 160863.0 58 139822.5 59 118782.0 60 118782.0 61 118782.0 62 97732.0 63 76682.0 64 76682.0 65 76682.0 66 59206.5 67 41731.0 68 41731.0 69 41731.0 70 30260.0 71 18789.0 72 18789.0 73 18789.0 74 12978.5 75 7168.0 76 7168.0 77 7168.0 78 4814.5 79 2461.0 80 2461.0 81 2461.0 82 1628.0 83 795.0 84 795.0 85 795.0 86 530.0 87 265.0 88 265.0 89 265.0 90 169.5 91 74.0 92 74.0 93 74.0 94 46.0 95 18.0 96 18.0 97 18.0 98 14.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.370571566357256 3 0.0 4 0.0 5 0.0 6 1.3285134864046572E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.314053945618629E-4 19 6.642567432023286E-4 20 5.314053945618629E-4 21 0.003586986413292574 22 0.0019263445552867532 23 9.96385114803493E-4 24 0.0 25 3.985540459213972E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1505442.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.7495404883923 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56596174230397 57.80570734015704 2 12.996549084399048 18.64992849480935 3 3.541500598337029 7.623031217101447 4 1.3269047537295078 3.8081922540782216 5 0.5977833863103057 2.144534163967977 6 0.3017173818599861 1.2988850103488876 7 0.18036687310491303 0.9058868185223973 8 0.10560350767715937 0.6061602519838876 9 0.07304276987323646 0.4716706656963691 >10 0.285354904274223 3.457487035277076 >50 0.014166114793832748 0.703546667398031 >100 0.009550862381003387 1.3577121373053103 >500 8.426367876792443E-4 0.4339598477248011 >1k 6.553841681949678E-4 0.7332980956290911 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3005 0.19960915133229976 No Hit TATCAACGCAGAGTACTTTTTTTTT 1840 0.12222324074922847 No Hit GGTATCAACGCAGAGTACTTTTTTT 1804 0.11983191647370008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 6.642567432023286E-5 0.0 0.0 0.0 0.0 11 6.642567432023286E-5 0.0 0.0 0.0 0.0 12 6.642567432023286E-5 0.0 0.0 0.0 0.0 13 6.642567432023286E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTAGA 55 0.002436952 12.4682255 1 GTCCTAT 130 1.6116246E-9 12.057185 1 GGTATCA 985 0.0 11.536951 1 GTCTTAG 190 0.0 11.343273 1 TAGGACT 150 2.2610038E-9 10.728365 4 CTTATAC 125 2.3517896E-7 10.60215 3 GGACTAA 65 0.00847494 10.550037 1 CTAACCT 100 2.4944082E-5 10.412826 4 ATAGGAC 130 4.031972E-7 10.194374 3 GTATTGG 125 1.7333768E-6 10.188322 1 GTATAGG 100 2.0222634E-4 9.796464 1 GTATTAA 140 6.954524E-7 9.796462 1 CCAGTAC 155 4.2902684E-8 9.771566 3 TCTATAC 155 4.2902684E-8 9.771566 3 CTATACT 165 1.0824806E-8 9.75306 4 TAGCACC 110 7.105927E-5 9.466206 4 AGGATAG 120 1.772933E-5 9.466206 5 GGGGGCG 120 1.772933E-5 9.466206 7 TTAGACC 80 0.004649987 9.466205 4 ATAGCCC 150 2.786819E-7 9.466205 3 >>END_MODULE