FastQCFastQC Report
Thu 26 May 2016
SRR1511974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505442
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37590.2496941097697553No Hit
TATCAACGCAGAGTACTTTTTTTTT25750.17104611137459963No Hit
GGTATCAACGCAGAGTACTTTTTTT23600.15676459139574955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC551.968361E-413.8113218
TCCTATA957.337985E-813.0065052
TATGTCG853.921081E-612.3002715
GACCGTC550.00307980112.0849067
TAGGACA3850.011.6032074
GGTATCA9900.011.0817731
GTACTAG700.001440024810.9028121
AATACTA1408.281859E-910.8625765
TAGACAG1855.456968E-1210.7891815
AATCCCG803.784381E-410.68325919
TAGTACT1803.45608E-1110.5608384
CTAGGAC1659.2040864E-1010.3688233
TTAGGAC5000.010.2651353
TGGCGAG7400.010.13719418
CCGTCCC750.002658416510.1283039
GCTATAC1151.0199859E-59.9180043
TAGGACC2600.09.8703214
GTATAAG2159.094947E-129.761821
AGGACGT7750.09.5660755
TAACACC1106.796981E-59.5047544
Produced by FastQC (version 0.11.5)