FastQCFastQC Report
Thu 26 May 2016
SRR1511973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919835
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21870.23776003304940557No Hit
GTCCTACAGTGGACATTTCTAAATT13360.1452434403996369No Hit
TATCAACGCAGAGTACTTTTTTTTT12130.13187147694967033No Hit
GGTATCAACGCAGAGTACTTTTTTT11690.12708801034968228No Hit
GTCCTAAAGTGTGTATTTCTCATTT11550.1255659982496861No Hit
CTGTAGGACGTGGAATATGGCAAGA11470.12469627704968826No Hit
CTTTAGGACGTGAAATATGGCGAGG10790.11730364684970675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACC456.9222134E-414.7312273
GGCCTAA500.001221780113.6651781
GTTAGGG655.61141E-513.11241153
CTAGTAC604.1994796E-412.6267663
TAGGACC2550.012.6267664
GTCTAGG708.462022E-512.5496531
CGGTTTC1001.5003025E-712.31109813
GGCGTGC550.003140125412.0528238
TTAGGGT951.0785461E-611.9621994
GGTATCA5650.011.92032051
GGCGAGG2950.011.87836719
GACGTGG3600.011.8375937
TCCAACG1052.8264367E-711.72549218
TGGCGAG7300.011.6760718
TTATGGT658.2329655E-411.6554784
GTAGGAC8300.011.6379233
GATATAC1950.011.5127881
GTATTGG854.0336854E-511.4833421
GGGTAGC854.0336854E-511.4833421
TAGGACA3550.011.4707964
Produced by FastQC (version 0.11.5)