##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511973_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 919835 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.41922953573195 31.0 30.0 34.0 25.0 34.0 2 27.62798871536743 31.0 27.0 34.0 10.0 34.0 3 29.53632771094816 31.0 30.0 34.0 16.0 34.0 4 33.9988965412275 37.0 35.0 37.0 30.0 37.0 5 34.43073594720792 37.0 35.0 37.0 32.0 37.0 6 34.709308734718725 37.0 35.0 37.0 32.0 37.0 7 34.798550827050505 37.0 35.0 37.0 32.0 37.0 8 34.8380274723184 37.0 35.0 37.0 32.0 37.0 9 36.56884441231308 39.0 37.0 39.0 33.0 39.0 10 36.501289905254744 39.0 37.0 39.0 33.0 39.0 11 36.517174275821205 39.0 37.0 39.0 33.0 39.0 12 36.4401539406524 39.0 37.0 39.0 33.0 39.0 13 36.44426228617089 39.0 37.0 39.0 33.0 39.0 14 37.790142797349525 40.0 38.0 41.0 33.0 41.0 15 37.746142514690135 40.0 38.0 41.0 33.0 41.0 16 37.70739208662423 40.0 38.0 41.0 33.0 41.0 17 37.6807677463893 40.0 38.0 41.0 33.0 41.0 18 37.64691819728538 40.0 38.0 41.0 33.0 41.0 19 37.67520479216381 40.0 38.0 41.0 33.0 41.0 20 37.60279615365799 40.0 38.0 41.0 33.0 41.0 21 37.5600384851631 40.0 38.0 41.0 33.0 41.0 22 37.4801339370648 40.0 38.0 41.0 33.0 41.0 23 37.41709763164046 40.0 38.0 41.0 33.0 41.0 24 37.35650959139411 40.0 38.0 41.0 32.0 41.0 25 37.27247604189882 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3075963705740499 1101 2 4.195128260331149 1101 3 2.374469349215339 1101 4 1.3499079708864343 1101 5 0.9030699297395302 1101 6 0.666713717042775 1101 7 0.31484230809761726 1101 8 0.24083370805801962 1101 9 0.38465084889711676 1101 10 0.30773665120109683 1101 11 0.29308168681447455 1101 12 0.3526823431205486 1101 13 0.4696674593998864 1101 14 0.42982293257093573 1101 15 0.5993462755521364 1101 16 0.6242945957251109 1101 17 0.6144680615107703 1101 18 0.7271666442579772 1101 19 0.6658915492231259 1101 20 0.5834705660840172 1101 21 0.5751000285341163 1101 22 0.5196845724356365 1101 23 0.6350588695782164 1101 24 0.6819468612260664 1101 25 0.48395953007441506 1102 1 1.464829519347866 1102 2 4.372642616149911 1102 3 2.5174997651379876 1102 4 1.5699448827903097 1102 5 1.1292827341449794 1102 6 0.8431714589078112 1102 7 0.7155484302494699 1102 8 0.6520845591300528 1102 9 0.7757971305152509 1102 10 0.6738646267416044 1102 11 0.7015480871330695 1102 12 0.6879147981903699 1102 13 0.7660685487720826 1102 14 0.8908545389238895 1102 15 0.7583832688163525 1102 16 0.6657594673256426 1102 17 0.7107676645579275 1102 18 0.7291107282903653 1102 19 0.719649326629785 1102 20 0.9703218779125251 1102 21 0.7723699607481009 1102 22 0.7641060361510625 1102 23 0.7429255585119208 1102 24 0.7630674072824419 1102 25 0.8008516505391086 1103 1 1.2676397122509364 1103 2 4.199029851205875 1103 3 2.3075291372107003 1103 4 1.4758550201245981 1103 5 1.0441235692644497 1103 6 0.860377936640603 1103 7 0.6301908054181595 1103 8 0.6473215203112801 1103 9 0.6863710766929074 1103 10 0.6151601733003105 1103 11 0.6421291778689593 1103 12 0.599679504072725 1103 13 0.6427653813512677 1103 14 0.5107441951912577 1103 15 0.7804321693482237 1103 16 0.628607764539268 1103 17 0.6639708081635476 1103 18 0.7644564455246297 1103 19 0.7001407134679241 1103 20 0.8674005474370148 1103 21 0.8270655231835988 1103 22 0.8407909611332016 1103 23 0.9758580027446726 1103 24 0.9846876343206219 1103 25 0.806964217023193 1104 1 1.4633931421241506 1104 2 4.186334137965698 1104 3 2.521977121168664 1104 4 1.7234690213683592 1104 5 1.2662044224705298 1104 6 0.9288008929945946 1104 7 0.819546670363863 1104 8 0.84657779942237 1104 9 0.8652892547036473 1104 10 0.8321993349855674 1104 11 0.8007875189229381 1104 12 0.7443476006464422 1104 13 0.7838809203796941 1104 14 0.8820324652998508 1104 15 0.9866507843690187 1104 16 0.8495284046976579 1104 17 0.9210092929697282 1104 18 0.761610380518853 1104 19 0.9121529903622303 1104 20 0.793081339723976 1104 21 0.8104016063381962 1104 22 0.96714313285743 1104 23 1.0798530699883813 1104 24 1.110945677547818 1104 25 1.0853222775954094 1105 1 1.488471512030106 1105 2 4.343273543645903 1105 3 2.6766035873022993 1105 4 1.5650737552629082 1105 5 1.2233974296441659 1105 6 0.9363384929382406 1105 7 0.8683179946098107 1105 8 0.7081536509246078 1105 9 0.7034069680635184 1105 10 0.6717142047212832 1105 11 0.7235329899055287 1105 12 0.6872285605750648 1105 13 0.7185315725816821 1105 14 0.8948064664504543 1105 15 0.44441322082188606 1105 16 0.8383276720711166 1105 17 0.9994966070246534 1105 18 0.860620630245883 1105 19 0.8643326717166531 1105 20 0.8087788025349596 1105 21 0.7287912368549101 1105 22 0.9941822180135205 1105 23 1.0043831440728894 1105 24 0.9156725276059348 1105 25 1.0061305149476212 1106 1 0.18146393627252877 1106 2 2.1067854180074974 1106 3 0.9020972883578011 1106 4 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-0.7406488006781871 2315 14 -0.7835273826494387 2315 15 -0.9888516280197308 2315 16 -0.9972164771583749 2315 17 -0.9071791185192879 2315 18 -0.8680516470302209 2315 19 -0.9106120094116434 2315 20 -0.9169547687490152 2315 21 -0.84341763231334 2315 22 -1.019408424662636 2315 23 -0.9558210064130535 2315 24 -0.7909687617949501 2315 25 -0.7715958154392482 2316 1 -0.5967491768985802 2316 2 -0.1251851787480085 2316 3 -0.12269027440774849 2316 4 -0.500565385109006 2316 5 -0.8045131419538336 2316 6 -0.9003182038353756 2316 7 -1.0218222269845754 2316 8 -1.1206285268361782 2316 9 -1.2033202833588845 2316 10 -1.433533067958642 2316 11 -1.3004752385111544 2316 12 -1.417854191115424 2316 13 -1.473896437649394 2316 14 -1.5858670517066287 2316 15 -1.4710573151259538 2316 16 -1.6274146785905756 2316 17 -1.6566202005106447 2316 18 -1.5118288505661184 2316 19 -1.3717028695514841 2316 20 -1.3608518064175854 2316 21 -1.3274749415355558 2316 22 -1.4882607884832808 2316 23 -1.5507592020911076 2316 24 -1.7908658205796613 2316 25 -1.5827646733460625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22190.0 3 1.0 4 182.0 5 838.0 6 978.0 7 916.0 8 822.0 9 811.0 10 766.0 11 727.0 12 692.0 13 712.0 14 646.0 15 751.0 16 746.0 17 783.0 18 886.0 19 1099.0 20 1444.0 21 1790.0 22 2190.0 23 2425.0 24 2908.0 25 3200.0 26 3854.0 27 4416.0 28 5228.0 29 6339.0 30 8345.0 31 11102.0 32 15569.0 33 22850.0 34 34518.0 35 56442.0 36 103099.0 37 156514.0 38 443056.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.98235009539755 18.978295020302554 13.098762278017254 26.94059260628265 2 16.253358942350577 21.22100776114586 39.9195870882144 22.60604620828916 3 17.373333260856565 24.46417020443884 30.4792707387738 27.683225795930795 4 11.565008941821088 16.820516723107946 39.072333625052316 32.54214071001865 5 12.534748079818664 37.38605293340653 36.64298488315839 13.43621410361641 6 30.148374598025296 37.74115764366179 18.776866260651378 13.333601497661535 7 26.422564916533943 31.641870552870895 23.42822354009143 18.50734099050373 8 23.811661874140473 36.84562992275788 21.553213348046114 17.789494855055526 9 25.19082226703702 15.225448042311937 20.89918300564775 38.68454668500329 10 13.9605472720651 28.60393440127849 35.44885767556138 21.986660651095033 11 32.77642185826806 22.530997407143673 25.630683763935924 19.061896970652345 12 22.128860067294678 24.42024928383895 32.80512265786798 20.645767990998383 13 27.65909103263085 21.941870009295144 26.771649263183072 23.627389694890933 14 20.086211113949783 21.790755950795525 28.549141965678626 29.573890969576066 15 21.93708654269516 30.53330216832367 24.35588991503911 23.173721373942065 16 21.296102018296757 27.91304962303022 28.556534595878606 22.234313762794415 17 20.68838432979828 28.313773665929215 28.761462653628094 22.23637935064441 18 20.568995826407242 27.399380973243996 30.512235399763654 21.519387800585108 19 22.30280509574637 26.734512831774705 28.5717081274067 22.390973945072222 20 22.253484071495897 27.22027681232055 29.121499757020054 21.404739359163496 21 21.880833437522085 26.584004218285394 28.227396024157294 23.307766320035224 22 22.006514360512018 27.59981909386028 28.425420791594824 21.968245754032882 23 21.22634330045748 27.401002800535757 28.755718485275445 22.616935413731323 24 21.85100789925139 26.64219848363944 29.333336232407152 22.173457384702022 25 21.605211403423898 27.135913305625287 28.970928292106468 22.287946998844347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 402.0 1 402.0 2 353.0 3 304.0 4 304.0 5 304.0 6 708.5 7 1113.0 8 1113.0 9 1113.0 10 1634.5 11 2156.0 12 2156.0 13 2156.0 14 3519.5 15 4883.0 16 4883.0 17 4883.0 18 8656.0 19 12429.0 20 12429.0 21 12429.0 22 19143.0 23 25857.0 24 25857.0 25 25857.0 26 36359.0 27 46861.0 28 46861.0 29 46861.0 30 60319.0 31 73777.0 32 73777.0 33 73777.0 34 82907.0 35 92037.0 36 92037.0 37 92037.0 38 98272.5 39 104508.0 40 104508.0 41 104508.0 42 109923.5 43 115339.0 44 115339.0 45 115339.0 46 116014.0 47 116689.0 48 116689.0 49 116689.0 50 112249.5 51 107810.0 52 107810.0 53 107810.0 54 96222.0 55 84634.0 56 84634.0 57 84634.0 58 72354.0 59 60074.0 60 60074.0 61 60074.0 62 48550.0 63 37026.0 64 37026.0 65 37026.0 66 28359.5 67 19693.0 68 19693.0 69 19693.0 70 14273.0 71 8853.0 72 8853.0 73 8853.0 74 6206.5 75 3560.0 76 3560.0 77 3560.0 78 2392.0 79 1224.0 80 1224.0 81 1224.0 82 823.0 83 422.0 84 422.0 85 422.0 86 271.5 87 121.0 88 121.0 89 121.0 90 78.0 91 35.0 92 35.0 93 35.0 94 27.5 95 20.0 96 20.0 97 20.0 98 14.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 2.983252431142542 3 0.0 4 0.0 5 0.0 6 1.087151499997282E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.348605999989128E-4 19 9.784363499975538E-4 20 6.522908999983693E-4 21 0.003261454499991846 22 0.0022830181499942925 23 0.0011958666499970103 24 1.087151499997282E-4 25 6.522908999983693E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 919835.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.37098381069676 #Duplication Level Percentage of deduplicated Percentage of total 1 87.1784843898119 68.32263588755029 2 9.162589342784646 14.361622820948764 3 2.077328014201443 4.884067205114645 4 0.6763355294990193 2.1202032333186667 5 0.29644611062119547 1.1616386668118865 6 0.158463711120342 0.745137416327715 7 0.09133855213510039 0.5010804533470704 8 0.061779942253061625 0.38734038833123785 9 0.051927962870921274 0.366268098373149 >10 0.21611453053077892 2.987716000717435 >50 0.01572115288129212 0.8778013219590504 >100 0.011927236888454588 1.967152150106472 >500 5.612816006500496E-4 0.29615049750840106 >1k 9.822428011375867E-4 1.0211858595852255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2187 0.23776003304940557 No Hit GTCCTACAGTGGACATTTCTAAATT 1336 0.1452434403996369 No Hit TATCAACGCAGAGTACTTTTTTTTT 1213 0.13187147694967033 No Hit GGTATCAACGCAGAGTACTTTTTTT 1169 0.12708801034968228 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1155 0.1255659982496861 No Hit CTGTAGGACGTGGAATATGGCAAGA 1147 0.12469627704968826 No Hit CTTTAGGACGTGAAATATGGCGAGG 1079 0.11730364684970675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.087151499997282E-4 0.0 5 0.0 0.0 0.0 1.087151499997282E-4 0.0 6 0.0 0.0 0.0 1.087151499997282E-4 0.0 7 0.0 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 8 0.0 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 9 0.0 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 10 0.0 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 11 0.0 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 12 1.087151499997282E-4 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 13 1.087151499997282E-4 0.0 0.0 1.087151499997282E-4 1.087151499997282E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAACC 45 6.9222134E-4 14.731227 3 GGCCTAA 50 0.0012217801 13.665178 1 GTTAGGG 65 5.61141E-5 13.1124115 3 CTAGTAC 60 4.1994796E-4 12.626766 3 TAGGACC 255 0.0 12.626766 4 GTCTAGG 70 8.462022E-5 12.549653 1 CGGTTTC 100 1.5003025E-7 12.311098 13 GGCGTGC 55 0.0031401254 12.052823 8 TTAGGGT 95 1.0785461E-6 11.962199 4 GGTATCA 565 0.0 11.9203205 1 GGCGAGG 295 0.0 11.878367 19 GACGTGG 360 0.0 11.837593 7 TCCAACG 105 2.8264367E-7 11.725492 18 TGGCGAG 730 0.0 11.67607 18 TTATGGT 65 8.2329655E-4 11.655478 4 GTAGGAC 830 0.0 11.637923 3 GATATAC 195 0.0 11.512788 1 GTATTGG 85 4.0336854E-5 11.483342 1 GGGTAGC 85 4.0336854E-5 11.483342 1 TAGGACA 355 0.0 11.470796 4 >>END_MODULE