FastQCFastQC Report
Thu 26 May 2016
SRR1511973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919835
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31040.3374518255991564No Hit
TATCAACGCAGAGTACTTTTTTTTT23170.25189300254937025No Hit
GGTATCAACGCAGAGTACTTTTTTT20870.22688851804943277No Hit
CTGTAGGACGTGGAATATGGCAAGA11270.1225219740496937No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10780.11719493169970702No Hit
GTCCTACAGTGGACATTTCTAAATT10330.11230274994971925No Hit
CTTTAGGACGTGAAATATGGCGAGG10200.11088945299972278No Hit
GTCCTAAAGTGTGTATTTCTCATTT9990.1086064348497285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG350.002166019516.2893965
TAGGACC3150.013.8761534
GGTATCA7500.011.1893561
AATCCCG855.328475E-511.17413619
GCAAAGT855.337336E-511.17231413
ACCTTTT2900.010.80632515
GTCCTAC6800.010.6583111
CTTACAC1252.228062E-710.6424063
CACCTTT3250.010.51916314
GTAGGAC7950.010.5181023
CCACCTT2650.010.3923613
TGTAGGA8550.010.335672
AGGACCT5350.010.3013945
CTGTAGG8350.010.2787361
TCCTACA7400.010.2725932
TTTGGAC750.002640853610.1356253
ACGAAAT750.002653991610.12956412
TAAGCTC856.5738056E-410.0610985
CGTGGGG856.610059E-410.05508210
TTTCCAC2750.010.01445610
Produced by FastQC (version 0.11.5)