##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511973_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 919835 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.86377556844434 31.0 29.0 33.0 24.0 34.0 2 29.05550343268086 31.0 29.0 33.0 25.0 34.0 3 29.05633292927536 31.0 28.0 34.0 24.0 34.0 4 32.57136334233857 35.0 32.0 37.0 26.0 37.0 5 32.07232818929482 35.0 32.0 37.0 23.0 37.0 6 32.06796545032533 35.0 32.0 37.0 23.0 37.0 7 31.825731788853435 35.0 32.0 37.0 19.0 37.0 8 31.678362967271305 35.0 32.0 37.0 19.0 37.0 9 32.827217924953935 37.0 32.0 39.0 18.0 39.0 10 32.46547369908733 37.0 32.0 39.0 17.0 39.0 11 32.56547206836009 37.0 32.0 39.0 17.0 39.0 12 32.24979371300287 37.0 32.0 39.0 16.0 39.0 13 32.2405866269494 37.0 32.0 39.0 15.0 39.0 14 32.78918719118103 38.0 32.0 40.0 11.0 41.0 15 32.690493403708274 37.0 32.0 40.0 10.0 41.0 16 32.522149081085196 37.0 31.0 40.0 10.0 41.0 17 32.40395831861149 37.0 31.0 40.0 10.0 41.0 18 32.234214832007915 37.0 31.0 40.0 10.0 41.0 19 32.26183500301685 37.0 31.0 40.0 8.0 41.0 20 32.06534867666484 37.0 31.0 40.0 2.0 41.0 21 31.866969619551334 37.0 31.0 40.0 2.0 41.0 22 31.654750036691365 37.0 31.0 40.0 2.0 41.0 23 31.678508645572304 37.0 31.0 40.0 2.0 41.0 24 31.445897362026884 37.0 31.0 40.0 2.0 41.0 25 30.810809547364475 37.0 30.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15971027696208395 1101 2 0.22418324807664547 1101 3 0.3437389998323148 1101 4 0.2081516715184364 1101 5 0.2798738388747495 1101 6 0.2839283648389994 1101 7 -0.2177625218495045 1101 8 0.13055808148349257 1101 9 0.020523407738949118 1101 10 0.19152108105515708 1101 11 0.19445066052151105 1101 12 0.23217871029360992 1101 13 -0.015358189595183092 1101 14 -0.1856343317621878 1101 15 -0.1802187786115681 1101 16 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0.045062848930040644 1103 3 0.1251827144492097 1103 4 0.21534168613364812 1103 5 0.23183261388440712 1103 6 0.3260983045302339 1103 7 -0.03492952169831298 1103 8 0.0804925649951791 1103 9 0.26355598119300794 1103 10 0.2013569538523754 1103 11 0.28947056745450084 1103 12 0.6429838911359198 1103 13 0.37138228111559357 1103 14 -0.2035379721270658 1103 15 0.14761984923797655 1103 16 0.41892311279924854 1103 17 0.42747354091562073 1103 18 0.1387735879421257 1103 19 0.2602597424779134 1103 20 0.12514101852610082 1103 21 0.05907538361782727 1103 22 -0.07866297258498278 1103 23 0.3354788625743801 1103 24 0.47165494501607697 1103 25 0.5023673313207873 1104 1 -0.2767147112228123 1104 2 -0.012981647408466301 1104 3 -0.05448743391422184 1104 4 -0.02851352097019344 1104 5 -0.12402492845226476 1104 6 -0.09985112414151587 1104 7 0.16413955804227598 1104 8 -0.13697704947988143 1104 9 -0.13581642498520807 1104 10 0.043923340883367246 1104 11 0.09996451996297395 1104 12 0.13556043135311313 1104 13 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23 -0.294677213233868 2314 24 -0.6073804360788024 2314 25 -0.5591975340198196 2315 1 -0.9482704222883811 2315 2 -0.7986194249164811 2315 3 -1.0735782715974729 2315 4 -1.1270287177101999 2315 5 -0.9669007053080918 2315 6 -0.9308161034451992 2315 7 -1.2279634689283974 2315 8 -1.1834146403758794 2315 9 -1.2910745279693323 2315 10 -1.1258543194684805 2315 11 -0.9928904296589209 2315 12 -1.1203884217208682 2315 13 -1.156425212912982 2315 14 -1.3489203741633489 2315 15 -1.5561580597011861 2315 16 -1.6756214446694209 2315 17 -1.3009855075290417 2315 18 -1.4243990624502807 2315 19 -1.1130445099175716 2315 20 -1.399104368025963 2315 21 -1.3958783666242596 2315 22 -1.5089079164673826 2315 23 -1.2543669408249514 2315 24 -1.4362328751124842 2315 25 -1.3627682823761482 2316 1 -1.0292534844991899 2316 2 -0.9874782819522494 2316 3 -1.0180177302850524 2316 4 -1.3404905251995665 2316 5 -1.2721280074299983 2316 6 -1.4295253789765994 2316 7 -1.5843393574562583 2316 8 -1.5297867543925143 2316 9 -1.812507416895759 2316 10 -1.8727654231302857 2316 11 -2.007791830451307 2316 12 -1.9647808969819813 2316 13 -1.903155509568446 2316 14 -2.174260806041339 2316 15 -1.2924899738534918 2316 16 -1.7603275474997773 2316 17 -1.7552756486640888 2316 18 -1.6693292075973964 2316 19 -1.524372530780326 2316 20 -1.9596681163531713 2316 21 -1.6359227633227498 2316 22 -1.6600322193046004 2316 23 -1.842500458220698 2316 24 -1.8077160468633018 2316 25 -2.1909712718946217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38946.0 3 1.0 4 631.0 5 4495.0 6 5621.0 7 4667.0 8 3907.0 9 3437.0 10 3259.0 11 3043.0 12 2957.0 13 2904.0 14 2867.0 15 2770.0 16 2867.0 17 3051.0 18 3201.0 19 3674.0 20 4337.0 21 5404.0 22 6860.0 23 8559.0 24 10225.0 25 11856.0 26 13542.0 27 15350.0 28 18081.0 29 21607.0 30 26862.0 31 34156.0 32 43002.0 33 54713.0 34 69846.0 35 91168.0 36 119056.0 37 148788.0 38 124125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22694820289292 22.89922798715713 15.13424554679332 20.73957826315663 2 17.560432034006098 24.637027292938406 38.058347421004854 19.74419325205064 3 20.89244266634777 27.754869079780615 30.626471051873434 20.726217201998185 4 15.810987840210473 20.466822854098833 37.030119532307424 26.69206977338327 5 13.345288612324884 39.83239409355568 35.34361820827415 11.478699085845284 6 30.941326050615757 39.88875801169629 17.58674058146453 11.583175356223428 7 27.388281593981528 33.37489875901657 22.77147531894307 16.465344328058837 8 25.492071947686266 38.2770823028043 20.399636891399002 15.83120885811042 9 27.298482880081753 15.698902520560754 20.38419934009904 36.618415259258455 10 15.225121896861937 30.89075758152277 34.2395103469644 19.64461017465089 11 34.85494681111286 23.316464365891708 24.613870965988465 17.214717857006963 12 23.312876765941716 26.24862067653438 31.81266205352047 18.625840504003435 13 29.54508145482614 22.89997662624275 26.008686340484978 21.546255578446136 14 21.492006718596272 23.176439252692056 28.097539232579756 27.234014796131916 15 23.505846157191236 31.950404148570122 23.481276533291297 21.06247316094734 16 22.45533166274386 29.42495121407644 27.560268961281096 20.5594481618986 17 21.997532166095006 29.728918773475677 27.672897856680816 20.600651203748498 18 21.77270923589557 28.53935760217865 29.841873814325393 19.846059347600384 19 23.98810656259003 27.8396668967804 27.954143949730113 20.218082590899456 20 23.92844795908827 28.180410813255435 28.377294163946974 19.51384706370932 21 23.59107883479102 27.63658699658091 27.630607663330924 21.141726505297147 22 23.681312409290797 28.473476221278816 27.870107138780327 19.97510423065006 23 22.90856512309273 28.476302815178812 28.051661439279872 20.56347062244859 24 23.208504291066276 27.971946624223488 28.565216162327765 20.25433292238247 25 23.254347414818504 28.25390163763316 28.325334713430507 20.16641623411783 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 432.0 1 432.0 2 396.0 3 360.0 4 360.0 5 360.0 6 895.0 7 1430.0 8 1430.0 9 1430.0 10 2064.0 11 2698.0 12 2698.0 13 2698.0 14 4273.0 15 5848.0 16 5848.0 17 5848.0 18 10137.5 19 14427.0 20 14427.0 21 14427.0 22 22180.5 23 29934.0 24 29934.0 25 29934.0 26 41724.5 27 53515.0 28 53515.0 29 53515.0 30 66875.0 31 80235.0 32 80235.0 33 80235.0 34 88934.5 35 97634.0 36 97634.0 37 97634.0 38 102552.5 39 107471.0 40 107471.0 41 107471.0 42 111090.5 43 114710.0 44 114710.0 45 114710.0 46 113811.0 47 112912.0 48 112912.0 49 112912.0 50 107875.0 51 102838.0 52 102838.0 53 102838.0 54 90380.0 55 77922.0 56 77922.0 57 77922.0 58 65930.0 59 53938.0 60 53938.0 61 53938.0 62 43365.0 63 32792.0 64 32792.0 65 32792.0 66 25229.5 67 17667.0 68 17667.0 69 17667.0 70 12874.5 71 8082.0 72 8082.0 73 8082.0 74 5706.5 75 3331.0 76 3331.0 77 3331.0 78 2258.0 79 1185.0 80 1185.0 81 1185.0 82 765.5 83 346.0 84 346.0 85 346.0 86 227.0 87 108.0 88 108.0 89 108.0 90 61.0 91 14.0 92 14.0 93 14.0 94 9.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38365576434904086 2 0.0 3 0.0 4 0.0 5 0.03228839954991928 6 0.04326862969989183 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.087151499997282E-4 21 0.0 22 0.0 23 0.0 24 0.0018481575499953795 25 0.010110508949974725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 919835.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.00815316529403 #Duplication Level Percentage of deduplicated Percentage of total 1 88.6178897880924 70.0153580956275 2 8.094609812375825 12.790803437389622 3 1.772998058980492 4.202439066170991 4 0.6426137508295473 2.0308690260665987 5 0.26842814153894995 1.060400586029229 6 0.16004816968434038 0.7587066182547199 7 0.08773847038419233 0.48524381546220174 8 0.06612705539243342 0.41796612166522096 9 0.04518705314803872 0.32131310545876723 >10 0.2125052448590249 2.9342867158262496 >50 0.018127127644062345 1.0394400668327861 >100 0.011119135459734239 1.801580360192714 >500 0.001647278912210036 0.8654872896022989 >1k 9.609126987891877E-4 1.276105695421134 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3104 0.3374518255991564 No Hit TATCAACGCAGAGTACTTTTTTTTT 2317 0.25189300254937025 No Hit GGTATCAACGCAGAGTACTTTTTTT 2087 0.22688851804943277 No Hit CTGTAGGACGTGGAATATGGCAAGA 1127 0.1225219740496937 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1078 0.11719493169970702 No Hit GTCCTACAGTGGACATTTCTAAATT 1033 0.11230274994971925 No Hit CTTTAGGACGTGAAATATGGCGAGG 1020 0.11088945299972278 No Hit GTCCTAAAGTGTGTATTTCTCATTT 999 0.1086064348497285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.087151499997282E-4 0.0 0.0 0.0 6 0.0 1.087151499997282E-4 0.0 0.0 0.0 7 0.0 1.087151499997282E-4 0.0 0.0 0.0 8 0.0 1.087151499997282E-4 0.0 0.0 0.0 9 0.0 1.087151499997282E-4 0.0 0.0 0.0 10 0.0 1.087151499997282E-4 0.0 0.0 0.0 11 0.0 1.087151499997282E-4 0.0 0.0 0.0 12 0.0 1.087151499997282E-4 0.0 0.0 0.0 13 0.0 2.174302999994564E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 35 0.0021660195 16.289396 5 TAGGACC 315 0.0 13.876153 4 GGTATCA 750 0.0 11.189356 1 AATCCCG 85 5.328475E-5 11.174136 19 GCAAAGT 85 5.337336E-5 11.172314 13 ACCTTTT 290 0.0 10.806325 15 GTCCTAC 680 0.0 10.658311 1 CTTACAC 125 2.228062E-7 10.642406 3 CACCTTT 325 0.0 10.519163 14 GTAGGAC 795 0.0 10.518102 3 CCACCTT 265 0.0 10.39236 13 TGTAGGA 855 0.0 10.33567 2 AGGACCT 535 0.0 10.301394 5 CTGTAGG 835 0.0 10.278736 1 TCCTACA 740 0.0 10.272593 2 TTTGGAC 75 0.0026408536 10.135625 3 ACGAAAT 75 0.0026539916 10.129564 12 TAAGCTC 85 6.5738056E-4 10.061098 5 CGTGGGG 85 6.610059E-4 10.055082 10 TTTCCAC 275 0.0 10.014456 10 >>END_MODULE