FastQCFastQC Report
Thu 26 May 2016
SRR1511972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716800
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22060.3077566964285714No Hit
GGTATCAACGCAGAGTACTTTTTTT13860.193359375No Hit
TATCAACGCAGAGTACTTTTTTTTT13320.18582589285714285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACAC552.0109296E-413.7746543
GGTATCA4000.013.1789781
TATACTA701.1218738E-412.1758115
GTCCTAA1950.011.5143971
GTATAGA854.024953E-511.4849481
TTGGAGT855.4868484E-511.1412654
CCTCGAA600.0060098911.04842217
ATAAGAC600.0060098911.0484223
TTAGGAC2950.010.9146633
CCTAGAG1159.1539005E-710.7053033
ATTACAC803.876703E-410.6538343
GTATTGA1104.3546534E-610.6496781
GAATAAT650.00869025910.51314451
GTCCTAC4200.010.4595071
CTTATAC1002.4782117E-510.4170833
GTATTAG750.002109276410.413021
CTAGAGT1106.2606414E-610.3309914
GTATCAA8600.010.3297761
GACGTGA2700.010.1715637
GAACTAT750.002714711710.1014146
Produced by FastQC (version 0.11.5)