##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511972_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 716800 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.472892020089287 31.0 30.0 34.0 25.0 34.0 2 27.641982421875 31.0 27.0 34.0 10.0 34.0 3 29.557276785714286 31.0 30.0 34.0 16.0 34.0 4 34.05345284598214 37.0 35.0 37.0 30.0 37.0 5 34.48689592633929 37.0 35.0 37.0 32.0 37.0 6 34.77996930803572 37.0 35.0 37.0 32.0 37.0 7 34.87232840401786 37.0 35.0 37.0 32.0 37.0 8 34.925630580357144 37.0 35.0 37.0 33.0 37.0 9 36.66700753348214 39.0 37.0 39.0 33.0 39.0 10 36.606201171875 39.0 37.0 39.0 33.0 39.0 11 36.621611328125 39.0 37.0 39.0 33.0 39.0 12 36.54793387276786 39.0 37.0 39.0 33.0 39.0 13 36.54036411830357 39.0 37.0 39.0 33.0 39.0 14 37.89533482142857 40.0 38.0 41.0 33.0 41.0 15 37.842354910714285 40.0 38.0 41.0 33.0 41.0 16 37.79839425223214 40.0 38.0 41.0 33.0 41.0 17 37.77307756696428 40.0 38.0 41.0 33.0 41.0 18 37.74268415178572 40.0 38.0 41.0 33.0 41.0 19 37.76299107142857 40.0 38.0 41.0 33.0 41.0 20 37.68749302455357 40.0 38.0 41.0 33.0 41.0 21 37.64820033482143 40.0 38.0 41.0 33.0 41.0 22 37.57200474330357 40.0 38.0 41.0 33.0 41.0 23 37.49757952008929 40.0 38.0 41.0 33.0 41.0 24 37.43329380580357 40.0 38.0 41.0 33.0 41.0 25 37.340232979910716 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0285886817600307 1101 2 4.068260619725805 1101 3 2.0116013631910086 1101 4 0.9793061538653589 1101 5 0.8702226950924299 1101 6 0.40189215840010206 1101 7 0.16555983949717046 1101 8 0.062193572146519216 1101 9 0.25091152884610324 1101 10 0.2693116901294559 1101 11 0.33607638629435854 1101 12 0.21142491946432074 1101 13 0.28041690589198964 1101 14 0.13703479271233476 1101 15 0.2481700225107204 1101 16 0.16095679991622092 1101 17 0.20415439840877525 1101 18 0.22430554442400563 1101 19 8.992493904784737E-4 1101 20 0.07547138585476176 1101 21 0.16872931390782497 1101 22 0.17529968433377974 1101 23 0.31923160550944374 1101 24 0.318590028870112 1101 25 -0.00541731261932199 1102 1 1.653544371231849 1102 2 4.530545270558839 1102 3 2.6196144791073515 1102 4 1.6555202623375322 1102 5 1.225465516786869 1102 6 0.8951658202221537 1102 7 0.7680997189725289 1102 8 0.7611354367335394 1102 9 0.9286286504341916 1102 10 0.8405169364959946 1102 11 0.8400147060391276 1102 12 0.7845904635976098 1102 13 0.8567019112092495 1102 14 0.9513967211065193 1102 15 1.025318196916956 1102 16 0.9904729289484777 1102 17 0.9101327748710233 1102 18 1.057526033612234 1102 19 1.0754596889509145 1102 20 0.9814160863155905 1102 21 1.020852674418279 1102 22 0.9772280785202341 1102 23 0.9710217508479744 1102 24 1.095545009373474 1102 25 1.1225638997167309 1103 1 1.5433906583069756 1103 2 4.451627755945289 1103 3 2.7347649445760673 1103 4 1.744067566948921 1103 5 1.3025038367695458 1103 6 1.1727583727226971 1103 7 0.935157523692169 1103 8 0.9834432955774517 1103 9 0.9134825149070238 1103 10 0.9142818206700554 1103 11 0.9078995295940118 1103 12 0.9055690303869568 1103 13 1.0592132773058083 1103 14 1.1917604361963683 1103 15 1.1498386558907612 1103 16 0.9544131757551497 1103 17 0.9963938703462318 1103 18 1.1438737279183613 1103 19 1.1060637157965516 1103 20 1.2438798230551456 1103 21 1.2445461145568402 1103 22 1.1841646448765886 1103 23 1.0781445046907976 1103 24 1.4861576961022038 1103 25 1.3942157724164446 1104 1 1.3416189157747205 1104 2 4.052851805741859 1104 3 2.275415916513335 1104 4 1.4299979491167392 1104 5 1.0142470645054047 1104 6 0.7018765004828893 1104 7 0.7017082765523384 1104 8 0.5290913117126479 1104 9 0.4811825531585754 1104 10 0.4973763715620123 1104 11 0.6135346792966914 1104 12 0.5978196128538329 1104 13 0.605389860398617 1104 14 0.4712245666689512 1104 15 0.35093578006922144 1104 16 0.6667858154602655 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-0.4263533353610143 2315 14 -0.37798036454420014 2315 15 -1.1606180285187122 2315 16 -0.8376064184745857 2315 17 -0.856443050119708 2315 18 -0.6160853864841727 2315 19 -0.4733334144973611 2315 20 -0.45511153696953954 2315 21 -0.6637987901720024 2315 22 -0.6775012503637043 2315 23 -0.7989634178579905 2315 24 -0.7086930328957095 2315 25 -0.5039173757745559 2316 1 -0.3657228890221269 2316 2 0.39817335405030363 2316 3 0.2627595187609302 2316 4 -0.10343463475778236 2316 5 -0.36216256670459046 2316 6 -0.6968742712486744 2316 7 -0.5830665341292018 2316 8 -0.7478958482370217 2316 9 -0.7385304017723016 2316 10 -1.0510395272805866 2316 11 -0.9062744001740768 2316 12 -0.760887082259444 2316 13 -0.8735369833560185 2316 14 -1.138902505283788 2316 15 -0.9720680727273745 2316 16 -0.8950386751969006 2316 17 -0.908186691873496 2316 18 -1.0305156148089196 2316 19 -0.9982658012451608 2316 20 -1.0088315660952532 2316 21 -1.1512448295957327 2316 22 -1.136055627021534 2316 23 -0.9112144190542253 2316 24 -1.131366876969146 2316 25 -1.2145586614665547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15700.0 3 0.0 4 133.0 5 553.0 6 698.0 7 632.0 8 591.0 9 577.0 10 569.0 11 534.0 12 531.0 13 478.0 14 510.0 15 539.0 16 590.0 17 578.0 18 701.0 19 849.0 20 1108.0 21 1347.0 22 1607.0 23 1939.0 24 2224.0 25 2524.0 26 2964.0 27 3384.0 28 4158.0 29 4937.0 30 6582.0 31 8997.0 32 12372.0 33 18068.0 34 27725.0 35 45529.0 36 81795.0 37 123877.0 38 340900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02036830357142 18.411551339285715 12.959542410714286 25.608537946428573 2 16.83104951907589 21.176768374062313 38.454801187353304 23.53738091950849 3 17.685267857142858 24.825334821428573 30.355189732142858 27.134207589285715 4 12.364536830357142 16.888950892857142 38.49497767857143 32.251534598214285 5 13.594029017857142 36.984375 35.828543526785715 13.593052455357144 6 31.20717075892857 36.92103794642857 18.489118303571427 13.382672991071429 7 27.205357142857146 31.802455357142854 22.98032924107143 18.01185825892857 8 24.798549107142858 35.64327566964286 21.486886160714285 18.0712890625 9 25.84654017857143 15.505580357142856 20.713588169642858 37.93429129464286 10 15.076450892857144 28.182477678571427 34.489815848214285 22.251255580357142 11 33.23465401785714 22.662527901785715 25.027901785714285 19.074916294642858 12 22.688337053571427 24.530691964285715 31.869559151785715 20.911411830357142 13 27.92327008928571 21.805106026785715 26.543247767857142 23.728376116071427 14 20.9833984375 21.488978794642858 28.177176339285715 29.35044642857143 15 22.81529017857143 29.649972098214285 24.318777901785715 23.215959821428573 16 22.10435267857143 27.746372767857142 27.53111049107143 22.6181640625 17 21.452287946428573 27.85797991071429 28.1865234375 22.503208705357142 18 21.289988657876638 27.11827755975541 29.7074346014283 21.884299180939653 19 22.67933225260115 26.722451792555223 28.479990178544025 22.1182257762996 20 22.88326295098068 26.824535190478784 28.669403835663832 21.622798022876704 21 22.59887636217021 26.48375580199755 28.096805291449666 22.82056254438258 22 22.804523487985357 27.058895746604318 28.028633132066204 22.10794763334412 23 22.13346317018626 26.87678659369389 28.286111688525484 22.70363854759437 24 22.4521484375 26.462890625 28.656529017857142 22.428431919642858 25 22.345424107142858 26.682896205357142 28.488699776785715 22.482979910714285 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 756.0 1 756.0 2 601.0 3 446.0 4 446.0 5 446.0 6 832.0 7 1218.0 8 1218.0 9 1218.0 10 1602.0 11 1986.0 12 1986.0 13 1986.0 14 3026.5 15 4067.0 16 4067.0 17 4067.0 18 6791.5 19 9516.0 20 9516.0 21 9516.0 22 14213.5 23 18911.0 24 18911.0 25 18911.0 26 26138.5 27 33366.0 28 33366.0 29 33366.0 30 41885.0 31 50404.0 32 50404.0 33 50404.0 34 57685.0 35 64966.0 36 64966.0 37 64966.0 38 70791.0 39 76616.0 40 76616.0 41 76616.0 42 81820.0 43 87024.0 44 87024.0 45 87024.0 46 89647.0 47 92270.0 48 92270.0 49 92270.0 50 90371.5 51 88473.0 52 88473.0 53 88473.0 54 80406.5 55 72340.0 56 72340.0 57 72340.0 58 62063.5 59 51787.0 60 51787.0 61 51787.0 62 42220.0 63 32653.0 64 32653.0 65 32653.0 66 25048.5 67 17444.0 68 17444.0 69 17444.0 70 12689.5 71 7935.0 72 7935.0 73 7935.0 74 5497.0 75 3059.0 76 3059.0 77 3059.0 78 2063.0 79 1067.0 80 1067.0 81 1067.0 82 697.5 83 328.0 84 328.0 85 328.0 86 223.5 87 119.0 88 119.0 89 119.0 90 74.0 91 29.0 92 29.0 93 29.0 94 21.5 95 14.0 96 14.0 97 14.0 98 10.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 2.995535714285714 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 4.1852678571428575E-4 19 2.7901785714285713E-4 20 1.3950892857142856E-4 21 0.0029296875 22 0.0011160714285714285 23 4.1852678571428575E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 716800.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.28340168471006 #Duplication Level Percentage of deduplicated Percentage of total 1 84.9895110439498 65.68278520996675 2 10.709988605130604 16.554087028179524 3 2.4697341706618334 5.726083738971383 4 0.8413742104278357 2.600970442866008 5 0.35808460287638744 1.3836998100602869 6 0.18226482194486257 0.8451627268414184 7 0.11160402249214185 0.6037596949922731 8 0.06531857710378519 0.40384334654284293 9 0.05467075085279525 0.38026274387051523 >10 0.1945666782202367 2.596447987470242 >50 0.011716081822259092 0.6217905351166523 >100 0.009518927786735356 1.361386387428535 >500 0.0010983378204623626 0.5431115388332572 >1k 5.491689102311813E-4 0.6966088088603696 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2206 0.3077566964285714 No Hit GGTATCAACGCAGAGTACTTTTTTT 1386 0.193359375 No Hit TATCAACGCAGAGTACTTTTTTTTT 1332 0.18582589285714285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 1.3950892857142856E-4 0.0 0.0 2.7901785714285713E-4 13 0.0 4.185267857142857E-4 0.0 0.0 2.7901785714285713E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTACAC 55 2.0109296E-4 13.774654 3 GGTATCA 400 0.0 13.178978 1 TATACTA 70 1.1218738E-4 12.175811 5 GTCCTAA 195 0.0 11.514397 1 GTATAGA 85 4.024953E-5 11.484948 1 TTGGAGT 85 5.4868484E-5 11.141265 4 CCTCGAA 60 0.00600989 11.048422 17 ATAAGAC 60 0.00600989 11.048422 3 TTAGGAC 295 0.0 10.914663 3 CCTAGAG 115 9.1539005E-7 10.705303 3 ATTACAC 80 3.876703E-4 10.653834 3 GTATTGA 110 4.3546534E-6 10.649678 1 GAATAAT 65 0.008690259 10.5131445 1 GTCCTAC 420 0.0 10.459507 1 CTTATAC 100 2.4782117E-5 10.417083 3 GTATTAG 75 0.0021092764 10.41302 1 CTAGAGT 110 6.2606414E-6 10.330991 4 GTATCAA 860 0.0 10.329776 1 GACGTGA 270 0.0 10.171563 7 GAACTAT 75 0.0027147117 10.101414 6 >>END_MODULE