FastQCFastQC Report
Thu 26 May 2016
SRR1511972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716800
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30840.4302455357142857No Hit
TATCAACGCAGAGTACTTTTTTTTT22060.3077566964285714No Hit
GGTATCAACGCAGAGTACTTTTTTT21170.2953404017857143No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9690.1351841517857143No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9030.1259765625No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7320.10212053571428573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGC250.00604575118.9908649
GGTATCA6500.012.9093821
TAGGACC1155.2696123E-912.3965864
GGGCTAT701.0521955E-412.2597211
TCGAACT550.003071919312.08762419
CAGAACG550.003076738712.08509359
AGTGCAA550.003076738712.085093510
TTAGGAC2600.011.6972913
GGTACAC600.005851825711.08805753
TTATGCT600.005851825711.08805754
ATAAGCT600.005851825711.08805754
GTATAAT951.30250555E-511.0409181
TGTTAGG951.3508536E-511.0046882
ATAACTG700.001484415410.8617715
GGCTATA700.001484415410.8617712
TAAGGCT909.456786E-510.5600554
GATCTGC909.490318E-510.556376
GACAGTT1553.6707206E-910.4143447
GTATAAC750.002564348510.1710291
ATAAACC750.002635258210.1376523
Produced by FastQC (version 0.11.5)