##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511972_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 716800 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.10470703125 31.0 30.0 33.0 25.0 34.0 2 29.297498604910714 31.0 30.0 34.0 25.0 34.0 3 29.313123604910714 31.0 30.0 34.0 25.0 34.0 4 32.87212332589286 35.0 33.0 37.0 27.0 37.0 5 32.38204799107143 35.0 32.0 37.0 25.0 37.0 6 32.359820033482144 35.0 32.0 37.0 25.0 37.0 7 32.126025390625 35.0 32.0 37.0 23.0 37.0 8 31.97900390625 35.0 32.0 37.0 22.0 37.0 9 33.16902622767857 37.0 33.0 39.0 21.0 39.0 10 32.84068917410714 37.0 32.0 39.0 18.0 39.0 11 32.93075892857143 37.0 32.0 39.0 18.0 39.0 12 32.61945591517857 37.0 32.0 39.0 17.0 39.0 13 32.60399553571428 37.0 32.0 39.0 17.0 39.0 14 33.19652064732143 38.0 32.0 40.0 17.0 41.0 15 33.079239676339284 38.0 32.0 40.0 16.0 41.0 16 32.91685267857143 38.0 32.0 40.0 11.0 41.0 17 32.80409737723214 38.0 32.0 40.0 11.0 41.0 18 32.647575334821425 37.0 32.0 40.0 10.0 41.0 19 32.67387834821429 38.0 32.0 40.0 10.0 41.0 20 32.48232421875 37.0 31.0 40.0 10.0 41.0 21 32.31115652901786 37.0 31.0 40.0 8.0 41.0 22 32.100888671875 37.0 31.0 40.0 2.0 41.0 23 32.12744838169643 37.0 31.0 40.0 2.0 41.0 24 31.886042131696428 37.0 31.0 40.0 2.0 41.0 25 31.264172712053572 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1870858899933303 1101 2 0.11023677362266326 1101 3 0.14995684381819174 1101 4 0.12052843270836888 1101 5 0.021159580247903875 1101 6 -0.2320312579060939 1101 7 -0.23827924055387228 1101 8 -0.04576772438267085 1101 9 -0.23093667136281226 1101 10 -0.2443031157247617 1101 11 -0.4885903010602064 1101 12 -0.24567729242195924 1101 13 -0.6469134312904856 1101 14 -0.28089191800738433 1101 15 -0.47004895920689904 1101 16 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3 0.45311157499305565 1103 4 0.57584409020523 1103 5 0.8501550813910512 1103 6 0.7347767662844831 1103 7 0.6479513074216428 1103 8 0.9120997437196934 1103 9 1.0985234658154397 1103 10 0.7930687406068273 1103 11 1.015097286519996 1103 12 1.0279269556789288 1103 13 1.0302604015880448 1103 14 1.0041475391797192 1103 15 1.1710989868068182 1103 16 1.2054970273038208 1103 17 1.0085720366907793 1103 18 0.8360331989218963 1103 19 1.2808785016486866 1103 20 1.1393258015678143 1103 21 1.0942460322052625 1103 22 1.3666121637547377 1103 23 1.1770294302565247 1103 24 1.290575423933305 1103 25 1.7574786775499796 1104 1 -0.16767269339219482 1104 2 -0.132240308880327 1104 3 0.07211564356766331 1104 4 0.08755428250623964 1104 5 0.21732481397790337 1104 6 -0.0849749463420082 1104 7 -0.0066701760432508195 1104 8 -0.05850045797032877 1104 9 -0.05898427143114304 1104 10 1.3832366486354886E-4 1104 11 0.47764553564195467 1104 12 0.19220347965570284 1104 13 0.13659451154388336 1104 14 0.30007374845050805 1104 15 0.3696210857740283 1104 16 0.331885384921101 1104 17 0.4482754132599638 1104 18 0.8125345725005033 1104 19 0.6721745339190903 1104 20 0.341092327978771 1104 21 0.7370390912865332 1104 22 0.4305583333830043 1104 23 0.43707046374402836 1104 24 0.23303465125187017 1104 25 0.3760115565011901 1105 1 0.03888849914888226 1105 2 0.052390208423155116 1105 3 0.079652148513496 1105 4 0.06069855670202173 1105 5 0.2824864886336371 1105 6 0.10348616584662551 1105 7 0.3775461105655964 1105 8 0.4607772012799032 1105 9 0.12160784294640337 1105 10 0.20362395720231063 1105 11 0.17969729369797705 1105 12 0.060134542801641544 1105 13 -0.03226337546396252 1105 14 -0.055499663203363525 1105 15 0.32785166957608425 1105 16 0.2559384729485856 1105 17 0.10043938765801386 1105 18 0.29011208489971807 1105 19 0.12759278223584403 1105 20 0.40563057851214523 1105 21 0.32660435686521083 1105 22 0.3394829960412835 1105 23 -0.00319165146475342 1105 24 0.5733410988426826 1105 25 8.695543399852568E-4 1106 1 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2313 19 0.3585578955538935 2313 20 0.04778744125724188 2313 21 0.17037823005084363 2313 22 0.22565564691981166 2313 23 0.20409126170051195 2313 24 -0.28074599053852367 2313 25 0.06758715046934682 2314 1 -0.3935082234955196 2314 2 -0.4932832443710389 2314 3 -0.35867852665928623 2314 4 -0.5630153626775325 2314 5 -0.641763753728064 2314 6 -0.33806557446672514 2314 7 -0.5800953192120559 2314 8 -0.37350212448549414 2314 9 -0.5931402572506101 2314 10 -0.5406609976089598 2314 11 -0.20194419862463064 2314 12 -0.5103614386850523 2314 13 -0.32800719133161493 2314 14 -0.7026835766411494 2314 15 -0.5961093023725041 2314 16 -0.5057808878419863 2314 17 -0.4849579291634498 2314 18 -0.11655520721649282 2314 19 -0.5830977140583684 2314 20 -0.5541217434692776 2314 21 -0.6216631479038419 2314 22 -0.2718626586480255 2314 23 -0.08996495727830478 2314 24 0.23208019256630052 2314 25 -0.03220890830504075 2315 1 -0.5020482517270182 2315 2 -0.557535109666464 2315 3 -0.7432318640256916 2315 4 -0.642970998894512 2315 5 -1.2948692185395174 2315 6 -0.8092543221981217 2315 7 -0.9110088098351667 2315 8 -0.7972518724185456 2315 9 -1.0509442499910833 2315 10 -1.1263133468729265 2315 11 -0.9676227628512883 2315 12 -0.8654985631053016 2315 13 -0.8133116882059852 2315 14 -0.7500721630497011 2315 15 -0.7193700404245291 2315 16 -0.9074243643493425 2315 17 -1.0341109842148732 2315 18 -1.1825967478496864 2315 19 -1.0360620213783918 2315 20 -0.9059819975527645 2315 21 -1.0774001916626652 2315 22 -1.5800649171678884 2315 23 -0.944875221444466 2315 24 -0.9579530802709684 2315 25 -0.8717178818884221 2316 1 -1.0451647025513786 2316 2 -1.1252458809776407 2316 3 -1.1077193035638402 2316 4 -1.4523276327947592 2316 5 -1.3838716630186347 2316 6 -1.3140717664171895 2316 7 -1.6282813952619115 2316 8 -1.4170508530187398 2316 9 -1.3599498150818405 2316 10 -1.484364524903821 2316 11 -1.863186293303258 2316 12 -1.7108787576234263 2316 13 -1.2890164263346584 2316 14 -1.7034840317759716 2316 15 -1.5536785648953249 2316 16 -1.9387345011361532 2316 17 -2.0420945489935676 2316 18 -1.663404986264414 2316 19 -1.5597786698414922 2316 20 -1.305153735213345 2316 21 -1.4130089709834124 2316 22 -1.1743659001605558 2316 23 -1.4866003995793804 2316 24 -1.6891708989634324 2316 25 -1.5329299794595457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27584.0 3 1.0 4 409.0 5 3274.0 6 3989.0 7 3400.0 8 2814.0 9 2535.0 10 2382.0 11 2277.0 12 2286.0 13 2106.0 14 2029.0 15 2038.0 16 2029.0 17 2203.0 18 2337.0 19 2638.0 20 3191.0 21 4002.0 22 4987.0 23 6270.0 24 7305.0 25 8669.0 26 9882.0 27 11379.0 28 13242.0 29 16174.0 30 19764.0 31 25435.0 32 32210.0 33 41464.0 34 54234.0 35 71231.0 36 95333.0 37 121544.0 38 104153.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.104797175116715 22.330556726676292 15.329698112309002 21.234947985897996 2 17.651925223214285 24.085379464285715 37.410435267857146 20.852260044642858 3 20.439592633928573 27.183314732142854 30.69321986607143 21.683872767857142 4 15.822684151785715 20.0625 36.38895089285714 27.725864955357142 5 13.790374913650542 39.05116772379337 34.95328407053094 12.205173292025147 6 31.28923575928609 38.922152345701754 17.423119544676776 12.365492350335378 7 27.57728794642857 33.161690848214285 22.362025669642858 16.89899553571429 8 25.96484375 36.99525669642857 20.388392857142858 16.651506696428573 9 27.28752790178571 15.607142857142858 20.077706473214285 37.027622767857146 10 15.735630580357144 30.134765625 33.609933035714285 20.51967075892857 11 34.924246651785715 23.232840401785715 23.978794642857142 17.864118303571427 12 23.4560546875 25.997488839285715 31.05678013392857 19.489676339285715 13 29.404854910714285 22.5400390625 25.847237723214285 22.20786830357143 14 21.976283482142858 22.47377232142857 27.636021205357142 27.913922991071427 15 23.74609375 30.892299107142858 23.703822544642858 21.657784598214285 16 22.9130859375 28.841238839285715 26.850306919642858 21.39536830357143 17 22.307896205357142 29.09361049107143 27.34430803571429 21.254185267857142 18 22.009517312384645 28.02905696017991 29.271943738760797 20.68948198867465 19 23.71051897321429 27.646763392857142 27.987723214285715 20.654994419642858 20 23.991277877449434 27.54862597272872 28.17279066068823 20.28730548913362 21 23.867745535714285 27.15987723214286 27.558314732142858 21.4140625 22 24.017299107142858 27.584263392857146 27.67340959821429 20.725027901785715 23 23.264927455357142 27.572265624999996 27.85853794642857 21.304268973214285 24 23.444547528198832 27.344182704716196 28.122937840496103 21.088331926588864 25 23.34923890446891 27.612629581571863 28.014175491468198 21.023956022491035 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 876.0 1 876.0 2 656.5 3 437.0 4 437.0 5 437.0 6 901.0 7 1365.0 8 1365.0 9 1365.0 10 1845.5 11 2326.0 12 2326.0 13 2326.0 14 3444.5 15 4563.0 16 4563.0 17 4563.0 18 7640.5 19 10718.0 20 10718.0 21 10718.0 22 15930.0 23 21142.0 24 21142.0 25 21142.0 26 29426.0 27 37710.0 28 37710.0 29 37710.0 30 46229.0 31 54748.0 32 54748.0 33 54748.0 34 61705.5 35 68663.0 36 68663.0 37 68663.0 38 73617.5 39 78572.0 40 78572.0 41 78572.0 42 82958.0 43 87344.0 44 87344.0 45 87344.0 46 88812.5 47 90281.0 48 90281.0 49 90281.0 50 87926.5 51 85572.0 52 85572.0 53 85572.0 54 76865.5 55 68159.0 56 68159.0 57 68159.0 58 57788.0 59 47417.0 60 47417.0 61 47417.0 62 38481.5 63 29546.0 64 29546.0 65 29546.0 66 22711.5 67 15877.0 68 15877.0 69 15877.0 70 11595.5 71 7314.0 72 7314.0 73 7314.0 74 5058.0 75 2802.0 76 2802.0 77 2802.0 78 1887.0 79 972.0 80 972.0 81 972.0 82 632.0 83 292.0 84 292.0 85 292.0 86 188.0 87 84.0 88 84.0 89 84.0 90 50.0 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3988560267857143 2 0.0 3 0.0 4 0.0 5 0.03278459821428571 6 0.04157366071428571 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.3950892857142856E-4 19 0.0 20 2.7901785714285713E-4 21 0.0 22 0.0 23 0.0 24 0.0020926339285714285 25 0.009765625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 716800.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.19977521021549 #Duplication Level Percentage of deduplicated Percentage of total 1 86.93173559736 67.9804218234744 2 9.223723921973255 14.425862745987917 3 2.177732182151874 5.108945013369858 4 0.7370080791296202 2.3053546446419606 5 0.3328067195831183 1.3012705329924537 6 0.15944822040401385 0.7481288995957667 7 0.10390096025259501 0.5687522215114926 8 0.06809181809506598 0.42598118949512354 9 0.039743023142216276 0.27971059283061345 >10 0.200255528772435 2.7102005462573886 >50 0.012866655901138847 0.681533410543777 >100 0.01072165626188757 1.6493337276656768 >500 0.0014295541619691442 0.7794614514294931 >1k 5.360828107384291E-4 1.0350432002040615 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3084 0.4302455357142857 No Hit TATCAACGCAGAGTACTTTTTTTTT 2206 0.3077566964285714 No Hit GGTATCAACGCAGAGTACTTTTTTT 2117 0.2953404017857143 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 969 0.1351841517857143 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 903 0.1259765625 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 732 0.10212053571428573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 1.3950892857142856E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGGC 25 0.006045751 18.990864 9 GGTATCA 650 0.0 12.909382 1 TAGGACC 115 5.2696123E-9 12.396586 4 GGGCTAT 70 1.0521955E-4 12.259721 1 TCGAACT 55 0.0030719193 12.087624 19 CAGAACG 55 0.0030767387 12.0850935 9 AGTGCAA 55 0.0030767387 12.0850935 10 TTAGGAC 260 0.0 11.697291 3 GGTACAC 60 0.0058518257 11.0880575 3 TTATGCT 60 0.0058518257 11.0880575 4 ATAAGCT 60 0.0058518257 11.0880575 4 GTATAAT 95 1.30250555E-5 11.040918 1 TGTTAGG 95 1.3508536E-5 11.004688 2 ATAACTG 70 0.0014844154 10.861771 5 GGCTATA 70 0.0014844154 10.861771 2 TAAGGCT 90 9.456786E-5 10.560055 4 GATCTGC 90 9.490318E-5 10.55637 6 GACAGTT 155 3.6707206E-9 10.414344 7 GTATAAC 75 0.0025643485 10.171029 1 ATAAACC 75 0.0026352582 10.137652 3 >>END_MODULE