FastQCFastQC Report
Thu 26 May 2016
SRR1511971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193923
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26840.22480511724792973No Hit
GGTATCAACGCAGAGTACTTTTTTT16250.13610592977939112No Hit
TATCAACGCAGAGTACTTTTTTTTT15710.13158302503595293No Hit
GTCCTACAGTGGACATTTCTAAATT14650.12270473053957416No Hit
GTCCTAAAGTGTGTATTTCTCATTT14090.11801431080563822No Hit
CTTTAGGACGTGAAATATGGCGAGG13540.11340764856695112No Hit
CTGTAGGACGTGGAATATGGCAAGA13200.11055989372849002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATT603.2335115E-413.0243811
GGCGAGG3850.012.54419319
TAGGACC3100.012.2183734
GGACCTG6250.011.3630886
TCGAACT855.49654E-511.14074819
GTAGTAC855.498885E-511.1402823
AGGACCT6400.011.09676555
GATATAC2500.010.9404791
ACTGTTC3300.010.9039738
GCCTCGA700.001531310110.82244216
TCCAACG1053.5988014E-610.82244218
GGCCTAC1104.3304262E-610.6563121
TGGCGAG9350.010.53307118
CTAGGAC1355.9562808E-810.5213783
GTAGTAA650.00865608210.5196911
TAGGACA2650.010.3625634
GGTATCA8400.010.34973051
AAGGACC3700.010.2370154
GATCTAT1053.0193836E-510.2334411
TATGGCG9850.010.09453616
Produced by FastQC (version 0.11.5)