##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511971_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193923 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.581324758799354 31.0 31.0 34.0 26.0 34.0 2 27.711770357049826 31.0 27.0 34.0 10.0 34.0 3 29.65537559792382 31.0 30.0 34.0 16.0 34.0 4 34.1792242883335 37.0 35.0 37.0 31.0 37.0 5 34.62987730364521 37.0 35.0 37.0 32.0 37.0 6 34.92102170743004 37.0 35.0 37.0 33.0 37.0 7 35.01838979565684 37.0 35.0 37.0 33.0 37.0 8 35.06267657126967 37.0 35.0 37.0 33.0 37.0 9 36.813118601450846 39.0 37.0 39.0 34.0 39.0 10 36.7449115227699 39.0 37.0 39.0 33.0 39.0 11 36.76387421969424 39.0 37.0 39.0 33.0 39.0 12 36.698488093453264 39.0 37.0 39.0 33.0 39.0 13 36.69587150930169 39.0 37.0 39.0 33.0 39.0 14 38.055636753794005 40.0 38.0 41.0 34.0 41.0 15 38.013342568993146 40.0 38.0 41.0 33.0 41.0 16 37.96990928225689 40.0 38.0 41.0 33.0 41.0 17 37.948290635158216 40.0 38.0 41.0 33.0 41.0 18 37.91371302839463 40.0 38.0 41.0 33.0 41.0 19 37.947745373864144 40.0 38.0 41.0 34.0 41.0 20 37.873686158990154 40.0 38.0 41.0 33.0 41.0 21 37.83008116938865 40.0 38.0 41.0 33.0 41.0 22 37.753955657106864 40.0 38.0 41.0 33.0 41.0 23 37.689283144725415 40.0 38.0 41.0 33.0 41.0 24 37.633184049557634 40.0 38.0 41.0 33.0 41.0 25 37.55168465638069 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.096451472205942 1101 2 4.075761109007274 1101 3 2.0645212146921885 1101 4 1.2215991411384906 1101 5 0.8239928318793801 1101 6 0.4206538203974901 1101 7 0.10036080730424857 1101 8 0.18109266058009865 1101 9 0.14735984652119782 1101 10 0.2840869649107418 1101 11 0.3454889354756929 1101 12 0.2958150384262197 1101 13 0.2552222538714588 1101 14 0.1739946365266647 1101 15 0.18808836035594823 1101 16 0.31897227154402685 1101 17 0.27665437696139605 1101 18 0.3725710945706311 1101 19 0.39901786862980515 1101 20 0.3506787744886992 1101 21 0.35552334186260026 1101 22 0.4189508667957753 1101 23 0.3669562990004067 1101 24 0.3447806551255965 1101 25 0.24100072895623725 1102 1 1.441235121051374 1102 2 4.2010770545034255 1102 3 2.4491113956782513 1102 4 1.5404414544316012 1102 5 1.0419503677645494 1102 6 0.8934727563242646 1102 7 0.7602773352842789 1102 8 0.6553626054521544 1102 9 0.7184033767895528 1102 10 0.5707190899367447 1102 11 0.8033206132372896 1102 12 0.7489387382389907 1102 13 0.8371477011358621 1102 14 0.7939421089626961 1102 15 0.8058931242419476 1102 16 0.7784278831520979 1102 17 0.7655995350641618 1102 18 0.7726484557106374 1102 19 0.6146012626385371 1102 20 0.6527759764857848 1102 21 0.7733658111781807 1102 22 0.6671195530866001 1102 23 0.765166591282501 1102 24 0.8210522382335625 1102 25 0.8154123942360414 1103 1 1.4040696418338356 1103 2 4.337232362778646 1103 3 2.4385993239781456 1103 4 1.657066791155252 1103 5 1.2458154517251785 1103 6 1.112705655798088 1103 7 0.9235568347933594 1103 8 0.9471471778445917 1103 9 0.9388554046841193 1103 10 0.9543486561611587 1103 11 0.8791117564612776 1103 12 0.8015192372193809 1103 13 0.8911644680853357 1103 14 0.9776528664964914 1103 15 1.0753872205637904 1103 16 1.0394560546482836 1103 17 1.0152093132671283 1103 18 0.9925164096896424 1103 19 1.0486103510300708 1103 20 1.1208451338584595 1103 21 1.0357005963044372 1103 22 1.0054147873455577 1103 23 1.1482743932008788 1103 24 1.1349038532482894 1103 25 1.2337251255368642 1104 1 1.241542931747798 1104 2 4.052712298828165 1104 3 2.3090855115969617 1104 4 1.4405568834427598 1104 5 1.0490300137822501 1104 6 0.8484064720890672 1104 7 0.6181247591186221 1104 8 0.6785673345437644 1104 9 0.6717000993052054 1104 10 0.683300852152982 1104 11 0.6743549271342459 1104 12 0.7641263978283419 1104 13 0.7340451092298821 1104 14 0.6997520358576566 1104 15 0.7940633995226491 1104 16 0.8586755308487639 1104 17 0.7792061669855315 1104 18 0.8008411172238041 1104 19 0.9673803035283939 1104 20 0.8506562800990665 1104 21 0.9217375625510869 1104 22 0.8364923887926423 1104 23 1.067842445632074 1104 24 0.9178027366770181 1104 25 1.0425347140093777 1105 1 1.310120597247849 1105 2 4.236913891441066 1105 3 2.396401871748843 1105 4 1.392735580964171 1105 5 0.9568027128716707 1105 6 0.7759222163076487 1105 7 0.6106587456213788 1105 8 0.4216694708382249 1105 9 0.5471356594611905 1105 10 0.5801448345051128 1105 11 0.4929652596459917 1105 12 0.5347994919964449 1105 13 0.601355550887881 1105 14 0.7515382168149642 1105 15 0.5602598320287626 1105 16 0.5060165642024259 1105 17 0.6226933897002525 1105 18 0.7074845625558837 1105 19 0.6744065293740178 1105 20 0.5491890829030481 1105 21 0.6519426345209993 1105 22 0.7227714752436469 1105 23 0.7247075142199861 1105 24 0.7137255092992447 1105 25 0.9590435968744444 1106 1 0.36172383741514125 1106 2 2.323163522180156 1106 3 1.1140319422841571 1106 4 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0.17491205929153608 1107 16 0.19382173407926473 1107 17 0.21254316593022082 1107 18 0.2143535896272155 1107 19 0.3004184586840708 1107 20 0.40546520849938616 1107 21 0.3437446069557879 1107 22 0.2986661734977787 1107 23 0.5097769109319188 1107 24 0.41140367250116583 1107 25 0.3967793954966936 1108 1 1.1233653478040289 1108 2 3.677784633728166 1108 3 2.1356934634960183 1108 4 1.3027394920156823 1108 5 0.864438406042062 1108 6 0.6358900644166283 1108 7 0.5239553049119081 1108 8 0.4830513926375133 1108 9 0.46240171254990514 1108 10 0.49772831259814154 1108 11 0.3945070774214656 1108 12 0.36372273380101916 1108 13 0.45829741336108754 1108 14 0.45459670989189505 1108 15 0.39293285796360067 1108 16 0.5163656128518426 1108 17 0.48964779801402614 1108 18 0.4261745634701555 1108 19 0.4776412737334681 1108 20 0.5451066054296874 1108 21 0.4517653673809434 1108 22 0.5154171746107679 1108 23 0.49571925753310353 1108 24 0.5938835259389919 1108 25 0.5771002106149297 1109 1 0.917778315161474 1109 2 3.139714535586645 1109 3 1.7176013099221876 1109 4 0.9794109768667454 1109 5 0.5719694601422844 1109 6 0.303060450360924 1109 7 0.08033965689187283 1109 8 0.1379508677903658 1109 9 0.074351067505809 1109 10 0.07840584723367527 1109 11 0.08084971474616509 1109 12 0.14322251865748825 1109 13 -0.0889143522844833 1109 14 0.13095514158575838 1109 15 0.18994300706966527 1109 16 0.26191265714049194 1109 17 0.3241856774460459 1109 18 0.20032410239309684 1109 19 0.3410521875652677 1109 20 0.32376514044397453 1109 21 0.3650277419986452 1109 22 0.24764979758397487 1109 23 0.21310697708900506 1109 24 0.32153114402959915 1109 25 0.3196146329609988 1110 1 1.247938421285962 1110 2 3.9491698595421454 1110 3 2.333744577978454 1110 4 1.279632945160742 1110 5 0.769223095037276 1110 6 0.6484391755536691 1110 7 0.6420087533889784 1110 8 0.46203377097803866 1110 9 0.4753203728369826 1110 10 0.5024585417186174 1110 11 0.42705852255314625 1110 12 0.31293501061278306 1110 13 0.3508403005124521 1110 14 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1112 1 0.8514647695078779 1112 2 3.6972665458879845 1112 3 2.0753252725140072 1112 4 1.1650266279484214 1112 5 0.5982369499680047 1112 6 0.4102297812347899 1112 7 0.31601435897070473 1112 8 0.17073832501624508 1112 9 0.27230488791440877 1112 10 0.24063414352352908 1112 11 0.2702552943741665 1112 12 0.1458926507026277 1112 13 0.07549889673166632 1112 14 0.0422575156070053 1112 15 0.1935064747127413 1112 16 0.21520707079766055 1112 17 0.1881636475400441 1112 18 0.24533320691138272 1112 19 0.17580616807646265 1112 20 0.30042650107901636 1112 21 0.30163843858974815 1112 22 0.36268732821215366 1112 23 0.23921976251861565 1112 24 0.3326441862026783 1112 25 0.31611238875978387 1113 1 0.4975675634458483 1113 2 3.1743648855227597 1113 3 1.5030696304195352 1113 4 0.616236134126865 1113 5 0.2823765520355934 1113 6 0.0937880287049353 1113 7 -0.020381737295018354 1113 8 -0.08671792564516068 1113 9 -0.285050622103995 1113 10 -0.08280722830839693 1113 11 -0.1995554973813185 1113 12 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0.48566068480177904 2313 25 0.5992383238763779 2314 1 0.4948125451996148 2314 2 2.093969410985938 2314 3 1.1587235519061245 2314 4 0.5410571111265412 2314 5 0.15812132884720143 2314 6 -0.050074982001696355 2314 7 -0.14234784879773343 2314 8 -0.20272653179740274 2314 9 -0.3419210064733562 2314 10 -0.40743772965789304 2314 11 -0.2598772778393439 2314 12 -0.2534793431859299 2314 13 -0.31431248894647723 2314 14 -0.26378202231978065 2314 15 -0.1249704905138671 2314 16 -0.44793365891500514 2314 17 -0.27731692244987727 2314 18 -0.18784912255122777 2314 19 -0.06227271722297445 2314 20 -0.07780906358841122 2314 21 -0.014370919126285742 2314 22 0.0026311764323523334 2314 23 -0.1512955767465698 2314 24 -0.17268481785036727 2314 25 -0.0805356406157145 2315 1 -2.308746234754423 2315 2 -10.91974478157682 2315 3 -4.453319807245158 2315 4 -1.6900082435324535 2315 5 -0.976402977003886 2315 6 -0.5482753233237361 2315 7 -0.30329936693065207 2315 8 -0.2440407803335063 2315 9 -0.36070021035340716 2315 10 -0.2129635113012327 2315 11 -0.562776784033197 2315 12 -0.28497877984587916 2315 13 -0.3907290980288778 2315 14 -0.5918659304138458 2315 15 -0.7401356623938966 2315 16 -0.6260616388107607 2315 17 -0.5280465235531864 2315 18 -0.564662305718258 2315 19 -0.48551060252664513 2315 20 -0.6363805601686963 2315 21 -0.7228456885607955 2315 22 -1.1228573281125946 2315 23 -0.9595183218355601 2315 24 -0.8177351433758062 2315 25 -0.566741383077705 2316 1 -0.0743380014782673 2316 2 0.47006958853943814 2316 3 0.5579071796044701 2316 4 0.2567277961092529 2316 5 -0.1329991271849451 2316 6 -0.353251881356897 2316 7 -0.3169505962045207 2316 8 -0.3901288306479742 2316 9 -0.2860129604963504 2316 10 -0.48069592982609777 2316 11 -0.42975448557693596 2316 12 -0.4680731194685208 2316 13 -0.5536366642571977 2316 14 -0.62258138686515 2316 15 -0.42928298467329284 2316 16 -0.3952813834267346 2316 17 -0.6288317341497134 2316 18 -0.6302571218036377 2316 19 -0.3608892951128908 2316 20 -0.3204308746341056 2316 21 -0.5329108686637127 2316 22 -0.6354153612743971 2316 23 -0.6703222097715198 2316 24 -0.5813509238217733 2316 25 -0.41578655174551216 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22274.0 3 1.0 4 166.0 5 907.0 6 1042.0 7 965.0 8 924.0 9 830.0 10 811.0 11 826.0 12 766.0 13 788.0 14 812.0 15 895.0 16 830.0 17 934.0 18 1133.0 19 1384.0 20 1661.0 21 2178.0 22 2584.0 23 3001.0 24 3550.0 25 4065.0 26 4742.0 27 5633.0 28 6592.0 29 8383.0 30 10961.0 31 14846.0 32 20808.0 33 30359.0 34 46133.0 35 75680.0 36 137955.0 37 205849.0 38 572655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.70084670451947 19.316321069281685 13.473565715711985 25.509266510486857 2 16.266798688764815 21.902275998762725 39.70167153109345 22.129253781379006 3 17.57441644059123 25.051029253980367 30.772755026915473 26.60179927851294 4 11.777141406941654 17.629026327493484 39.29859798328703 31.295234282277836 5 12.70500693930848 37.79866875837051 36.53300924766505 12.963315054655952 6 30.082836370946126 38.004472661484854 19.120627847761995 12.792063119807024 7 26.49107186979395 32.15466994102635 23.584351754677645 17.769906434502058 8 23.97357283509908 37.1010525804428 21.742021889183807 17.18335269527432 9 25.62996106114046 15.807719593307107 21.16510026190969 37.397219083642746 10 14.216255333262978 29.256182564690153 35.356497325620936 21.17106477642593 11 32.76408947645702 23.043445850360534 25.62183658410132 18.57062808908112 12 22.277232283824 25.034947814892583 32.783940002831 19.903879898452413 13 27.80824224007746 22.423640385519 26.776601171097298 22.991516203306244 14 20.378952411503924 22.431262317586643 28.65536554702439 28.53441972388504 15 22.153103675865193 30.90107150963672 24.427203429366887 22.5186213851312 16 21.35757498599156 28.600085600160146 28.38281865748461 21.659520756363683 17 20.90134790937104 28.72471675309044 28.75838726618048 21.615548071358035 18 20.639662956169225 28.050188037624274 30.51972091698704 20.790428089219457 19 22.466909064106332 27.434883956623086 28.50314136695739 21.595065612313185 20 22.408007987215093 27.837823349872853 29.05617518267739 20.697993480234658 21 22.203534211762893 27.276603122777715 28.114799480346736 22.405063185112652 22 22.058343147092472 28.195949081247875 28.36262942854606 21.38307834311359 23 21.536273496484636 27.847176736449054 28.559798040375174 22.056751726691136 24 21.861980933427812 27.42515842743496 29.1380006399078 21.57485999922943 25 21.94203267572036 27.692614375909823 28.7988917124684 21.566461235901414 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 418.0 1 418.0 2 461.5 3 505.0 4 505.0 5 505.0 6 1027.0 7 1549.0 8 1549.0 9 1549.0 10 2255.0 11 2961.0 12 2961.0 13 2961.0 14 4954.5 15 6948.0 16 6948.0 17 6948.0 18 12142.5 19 17337.0 20 17337.0 21 17337.0 22 26764.5 23 36192.0 24 36192.0 25 36192.0 26 50410.5 27 64629.0 28 64629.0 29 64629.0 30 82014.5 31 99400.0 32 99400.0 33 99400.0 34 111235.0 35 123070.0 36 123070.0 37 123070.0 38 131905.0 39 140740.0 40 140740.0 41 140740.0 42 147035.0 43 153330.0 44 153330.0 45 153330.0 46 151960.0 47 150590.0 48 150590.0 49 150590.0 50 144072.5 51 137555.0 52 137555.0 53 137555.0 54 121157.0 55 104759.0 56 104759.0 57 104759.0 58 88458.5 59 72158.0 60 72158.0 61 72158.0 62 57726.0 63 43294.0 64 43294.0 65 43294.0 66 33139.5 67 22985.0 68 22985.0 69 22985.0 70 16527.0 71 10069.0 72 10069.0 73 10069.0 74 6820.5 75 3572.0 76 3572.0 77 3572.0 78 2432.5 79 1293.0 80 1293.0 81 1293.0 82 840.0 83 387.0 84 387.0 85 387.0 86 253.5 87 120.0 88 120.0 89 120.0 90 80.0 91 40.0 92 40.0 93 40.0 94 26.5 95 13.0 96 13.0 97 13.0 98 11.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.06058263388845 3 0.0 4 0.0 5 0.0 6 2.5127248574656827E-4 7 0.0 8 0.0 9 0.0 10 8.375749524885608E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.001088847438235129 19 0.0013401199239816972 20 0.0012563624287328413 21 0.004187874762442804 22 0.0017589074002259779 23 0.0017589074002259779 24 8.375749524885608E-5 25 7.538174572397047E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1193923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.04492483183859 #Duplication Level Percentage of deduplicated Percentage of total 1 87.47929986113392 69.147946818652 2 9.162515062372197 14.485006287515986 3 1.9068387321146436 4.5217777273932125 4 0.6101752629461091 1.9292503117529016 5 0.2810290531438125 1.1106960190657718 6 0.1359091842142582 0.6445758750103527 7 0.09114403103978629 0.5043131157687469 8 0.06158798245736067 0.3894573955109335 9 0.042238501849844595 0.30048652833573974 >10 0.2017545758284557 2.7910391809023474 >50 0.014678915456931351 0.8175074433010582 >100 0.010904523560538018 1.783971315090658 >500 0.0010690632678409576 0.5235024388829681 >1k 8.55250614272766E-4 1.050469542817462 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2684 0.22480511724792973 No Hit GGTATCAACGCAGAGTACTTTTTTT 1625 0.13610592977939112 No Hit TATCAACGCAGAGTACTTTTTTTTT 1571 0.13158302503595293 No Hit GTCCTACAGTGGACATTTCTAAATT 1465 0.12270473053957416 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1409 0.11801431080563822 No Hit CTTTAGGACGTGAAATATGGCGAGG 1354 0.11340764856695112 No Hit CTGTAGGACGTGGAATATGGCAAGA 1320 0.11055989372849002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 8.375749524885608E-5 0.0 13 0.0 0.0 0.0 8.375749524885608E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATATT 60 3.2335115E-4 13.024381 1 GGCGAGG 385 0.0 12.544193 19 TAGGACC 310 0.0 12.218373 4 GGACCTG 625 0.0 11.363088 6 TCGAACT 85 5.49654E-5 11.140748 19 GTAGTAC 85 5.498885E-5 11.140282 3 AGGACCT 640 0.0 11.0967655 5 GATATAC 250 0.0 10.940479 1 ACTGTTC 330 0.0 10.903973 8 GCCTCGA 70 0.0015313101 10.822442 16 TCCAACG 105 3.5988014E-6 10.822442 18 GGCCTAC 110 4.3304262E-6 10.656312 1 TGGCGAG 935 0.0 10.533071 18 CTAGGAC 135 5.9562808E-8 10.521378 3 GTAGTAA 65 0.008656082 10.519691 1 TAGGACA 265 0.0 10.362563 4 GGTATCA 840 0.0 10.3497305 1 AAGGACC 370 0.0 10.237015 4 GATCTAT 105 3.0193836E-5 10.233441 1 TATGGCG 985 0.0 10.094536 16 >>END_MODULE