FastQCFastQC Report
Thu 26 May 2016
SRR1511971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193923
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32460.2718768295777868No Hit
TATCAACGCAGAGTACTTTTTTTTT23200.19431738897734613No Hit
GGTATCAACGCAGAGTACTTTTTTT22320.1869467293954468No Hit
CTTTAGGACGTGAAATATGGCGAGG14370.12035952067260619No Hit
CTGTAGGACGTGGAATATGGCAAGA13550.1134914060622No Hit
GTCCTACAGTGGACATTTCTAAATT13240.11089492370948545No Hit
GTCCTAAAGTGTGTATTTCTCATTT13180.11039237873799232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCG400.005292299714.2442528
TAGGGTG1001.9219715E-611.4030445
GGTATCA9550.011.2887811
ATTTAGA3150.010.903451
TTATGCT1408.303687E-910.8600414
TAGGACC3350.010.77899554
TGGACTG1553.6397978E-910.4221355
GTAACAC1409.636824E-810.1812893
CCCATAG750.002554958210.1765541
CTTAAGC750.002640947510.1360383
TCTTATA1701.54796E-910.0615082
CGAAATC856.615048E-410.05476613
ACGAAAT856.615048E-410.05476612
GGCGAGG4500.09.918220519
AACAGTA1356.470018E-79.84787757
TAGCACT1752.5556801E-99.7740374
GACGTGA5250.09.7674877
TTTAGAA4500.09.7137032
AGGACGT9000.09.7137035
TTAGGAC6900.09.6402533
Produced by FastQC (version 0.11.5)