##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511971_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193923 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.549385513136105 31.0 30.0 33.0 26.0 34.0 2 29.755108997816443 31.0 30.0 34.0 26.0 34.0 3 29.800482108142653 31.0 30.0 34.0 26.0 34.0 4 33.385067546232044 35.0 33.0 37.0 28.0 37.0 5 32.93661902819529 35.0 33.0 37.0 26.0 37.0 6 32.89628476878325 35.0 33.0 37.0 26.0 37.0 7 32.683028134980226 35.0 33.0 37.0 25.0 37.0 8 32.5441674211821 35.0 33.0 37.0 25.0 37.0 9 33.80884780676811 37.0 33.0 39.0 25.0 39.0 10 33.50773626104866 37.0 33.0 39.0 23.0 39.0 11 33.56027566266836 37.0 33.0 39.0 23.0 39.0 12 33.304363011685005 37.0 33.0 39.0 22.0 39.0 13 33.28209356884824 37.0 33.0 39.0 22.0 39.0 14 33.973267120241424 38.0 33.0 40.0 19.0 41.0 15 33.87906841563485 38.0 33.0 40.0 19.0 41.0 16 33.727053587207884 38.0 33.0 40.0 18.0 41.0 17 33.62006595065176 38.0 33.0 40.0 17.0 41.0 18 33.4751537578219 38.0 32.0 40.0 17.0 41.0 19 33.5029721347189 38.0 32.0 40.0 18.0 41.0 20 33.319232479816534 38.0 32.0 40.0 16.0 41.0 21 33.1630021366537 38.0 32.0 40.0 11.0 41.0 22 32.97348488972907 38.0 32.0 40.0 10.0 41.0 23 33.02183892931119 38.0 32.0 40.0 9.0 41.0 24 32.83377152462931 38.0 32.0 40.0 2.0 41.0 25 32.24056744027881 37.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14350130142117834 1101 2 0.2977120173183927 1101 3 0.32851395462565236 1101 4 0.29531991447390027 1101 5 0.48393100211460904 1101 6 0.31386590679453974 1101 7 0.09031022700067837 1101 8 0.38858143613239093 1101 9 0.18536647193769085 1101 10 0.04042943493022477 1101 11 0.2020475546636149 1101 12 0.3384802027067195 1101 13 0.0725133715869859 1101 14 0.24762817010567062 1101 15 0.055359026959521884 1101 16 0.5410188261096422 1101 17 0.29403240384203855 1101 18 0.29340570835189084 1101 19 0.4882279948662429 1101 20 0.5885894009190977 1101 21 0.6702659751077817 1101 22 0.6245519604898675 1101 23 0.0012285016043378505 1101 24 0.07037481133907875 1101 25 0.2703300390868506 1102 1 0.22758050882704595 1102 2 0.2623820298001931 1102 3 0.15412569271593668 1102 4 0.29472442872395277 1102 5 0.43303582320884715 1102 6 0.6728976573666188 1102 7 0.6448491806099952 1102 8 0.6500737910314953 1102 9 0.7061675172882218 1102 10 0.7548381097794064 1102 11 0.7056490732822951 1102 12 0.9273011929293133 1102 13 0.877009419017817 1102 14 1.0309168636775468 1102 15 0.9883857328051633 1102 16 0.933935406097163 1102 17 0.851310201844953 1102 18 0.8561423424271979 1102 19 0.8991794718789308 1102 20 1.086265966344513 1102 21 0.7900684766680044 1102 22 1.1979884853494482 1102 23 0.966738435034749 1102 24 1.1678023909105377 1102 25 1.3261626789869965 1103 1 0.7154402052093118 1103 2 0.5856247729335458 1103 3 0.7954149351821407 1103 4 0.8280260827608643 1103 5 0.6640087342634544 1103 6 0.5716653117559858 1103 7 0.7584609980365471 1103 8 0.6927948957368173 1103 9 0.7765131782735963 1103 10 0.8529713390535889 1103 11 0.9493866428298929 1103 12 1.170800853058715 1103 13 0.8555547728608701 1103 14 0.9127199401358439 1103 15 1.1025797359772938 1103 16 1.2480670996597638 1103 17 1.3848334011601722 1103 18 1.0301221012449489 1103 19 1.217139744782429 1103 20 1.0157705105402783 1103 21 1.5610271635087187 1103 22 1.1639365984382408 1103 23 1.4057813846080265 1103 24 1.5871731267933171 1103 25 1.5481334249400192 1104 1 0.15316727296709942 1104 2 0.2832851743662843 1104 3 0.3903913893125299 1104 4 0.5459259397946425 1104 5 0.2428367956108204 1104 6 0.45235129338047386 1104 7 0.29777862899923946 1104 8 0.6179355560460067 1104 9 0.7758726486324434 1104 10 0.8067391906146923 1104 11 0.6680122199471015 1104 12 1.0065344644673146 1104 13 0.8195467585540044 1104 14 1.007495267958916 1104 15 0.9683990246306919 1104 16 1.0960956775302293 1104 17 1.0870582168157412 1104 18 0.9487863663524791 1104 19 0.9854294281558182 1104 20 1.000547683088719 1104 21 1.142721595000232 1104 22 0.7812617722779862 1104 23 0.9583925677431324 1104 24 0.7923712810431027 1104 25 0.5029299671048051 1105 1 0.11352937718383416 1105 2 0.21540184299724174 1105 3 0.016752514799975415 1105 4 0.11385410823857 1105 5 0.03741637757555338 1105 6 0.11943601239293145 1105 7 0.07135908720852058 1105 8 0.27002419509987163 1105 9 0.41504821012073023 1105 10 0.25583758109181076 1105 11 0.11021466830759863 1105 12 0.3817881142052073 1105 13 0.3229114607589594 1105 14 0.4421785388619597 1105 15 0.5922294175059548 1105 16 0.40969130348141647 1105 17 0.2367442291856534 1105 18 0.3390392986971804 1105 19 0.2959614008333844 1105 20 0.694114459584867 1105 21 0.35959404248961135 1105 22 0.4967391462089381 1105 23 -0.23195746889516045 1105 24 0.048160387939816474 1105 25 -0.12304491900902548 1106 1 -0.3379141090537452 1106 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-1.1743029745970617 2315 4 -1.1760340170960148 2315 5 -1.2701187744494433 2315 6 -1.1921332919957486 2315 7 -1.129026574479866 2315 8 -1.0461227933521293 2315 9 -1.2716235185832048 2315 10 -1.2264580346012366 2315 11 -1.0915393822326536 2315 12 -1.1908880829301864 2315 13 -1.288014072666872 2315 14 -1.5865863058354677 2315 15 -1.6585230597968206 2315 16 -1.8670564650341674 2315 17 -1.6365486988933604 2315 18 -1.4888796577219594 2315 19 -1.3530168141975878 2315 20 -1.6684211840768413 2315 21 -1.827800119051286 2315 22 -2.222646908788821 2315 23 -1.7761282962651244 2315 24 -2.0680242926601977 2315 25 -1.8730244341459752 2316 1 -1.0346171196314522 2316 2 -1.0337163452547067 2316 3 -0.9066841447895513 2316 4 -1.096706401315572 2316 5 -1.0156282084117052 2316 6 -1.002232777415987 2316 7 -1.0556444513618963 2316 8 -0.9261159322886634 2316 9 -1.044293615781605 2316 10 -1.2646436644849786 2316 11 -1.254171743581999 2316 12 -1.4526513762406488 2316 13 -1.1653199617463486 2316 14 -1.3584902509469572 2316 15 -1.304466265438137 2316 16 -1.3964431621944513 2316 17 -1.1657051692573788 2316 18 -1.4316995548060056 2316 19 -1.0792763910986736 2316 20 -1.4703682014200865 2316 21 -1.261054931324967 2316 22 -1.2872148523756302 2316 23 -1.1272554165243207 2316 24 -1.4554914167310962 2316 25 -1.4244214229014531 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37593.0 3 4.0 4 651.0 5 4780.0 6 5898.0 7 5054.0 8 4062.0 9 3810.0 10 3487.0 11 3269.0 12 3259.0 13 2968.0 14 2924.0 15 3002.0 16 3023.0 17 3168.0 18 3372.0 19 3875.0 20 4773.0 21 5913.0 22 7570.0 23 9058.0 24 10948.0 25 12643.0 26 14492.0 27 16746.0 28 19512.0 29 23756.0 30 29399.0 31 37527.0 32 48485.0 33 63295.0 34 83219.0 35 114200.0 36 162353.0 37 229657.0 38 206178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.09898491674433 22.20936218318602 15.233903175121208 22.45774972494844 2 16.16879815532492 23.628491954673795 38.93961335865043 21.26309653135085 3 19.529818924671023 27.00216010580247 31.19656795287468 22.271453016651826 4 14.340288276547147 20.034709106031126 37.96233090408678 27.662671713334948 5 13.069860426612953 39.125709890456086 35.94060799635356 11.863821686577394 6 30.40939592816298 39.384665920319634 18.180309845747498 12.025628305769889 7 26.827274455722854 32.92096726505813 23.304769235536966 16.946989043682045 8 24.77915242440258 37.85068216292005 21.077741194365128 16.292424218312238 9 26.50028519427132 15.752104616461866 20.808125817159063 36.93948437210775 10 14.636119749766108 30.515870788987232 34.912134199609184 19.935875261637477 11 33.98058333745141 23.162716523594906 25.045166229312944 17.81153390964074 12 22.646937867852447 25.920348297168243 32.418589808555495 19.014124026423815 13 28.646235980042263 22.67776062610403 26.49668362197562 22.179319771878088 14 20.917513105954068 22.984061786229095 28.45903797816107 27.639387129655763 15 22.833465809771653 31.662259626458322 24.037396046478708 21.466878517291317 16 21.97109863868943 29.208667560638336 27.95088125448626 20.869352546185976 17 21.468386152205795 29.532222764784667 28.206090342509526 20.793300740500015 18 21.295510682012157 28.504267025595453 30.07530636397825 20.124915928414143 19 23.116649901208035 28.005491141388518 28.16898577211428 20.708873185289168 20 23.182586467122643 28.188859908771256 28.64550615534348 19.983047468762617 21 22.950977575605798 27.621211753186763 27.811508782392163 21.616301888815276 22 22.854572698574362 28.502759390680975 28.210194459776723 20.432473450967944 23 22.423891658004745 28.29797231479752 28.2369131007611 21.041222926436628 24 22.597091708916263 27.932811624453365 28.708087883628153 20.76200878300222 25 22.7199433079889 28.172026077654987 28.398526074723218 20.709504539632892 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 462.0 1 462.0 2 478.5 3 495.0 4 495.0 5 495.0 6 1087.5 7 1680.0 8 1680.0 9 1680.0 10 2446.5 11 3213.0 12 3213.0 13 3213.0 14 5351.0 15 7489.0 16 7489.0 17 7489.0 18 13101.0 19 18713.0 20 18713.0 21 18713.0 22 28884.0 23 39055.0 24 39055.0 25 39055.0 26 54197.0 27 69339.0 28 69339.0 29 69339.0 30 86749.5 31 104160.0 32 104160.0 33 104160.0 34 115933.0 35 127706.0 36 127706.0 37 127706.0 38 134803.5 39 141901.0 40 141901.0 41 141901.0 42 147503.5 43 153106.0 44 153106.0 45 153106.0 46 151119.0 47 149132.0 48 149132.0 49 149132.0 50 140836.5 51 132541.0 52 132541.0 53 132541.0 54 116280.0 55 100019.0 56 100019.0 57 100019.0 58 84184.5 59 68350.0 60 68350.0 61 68350.0 62 54676.5 63 41003.0 64 41003.0 65 41003.0 66 31092.5 67 21182.0 68 21182.0 69 21182.0 70 15295.0 71 9408.0 72 9408.0 73 9408.0 74 6378.5 75 3349.0 76 3349.0 77 3349.0 78 2271.0 79 1193.0 80 1193.0 81 1193.0 82 751.0 83 309.0 84 309.0 85 309.0 86 204.0 87 99.0 88 99.0 89 99.0 90 57.5 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4233941384829675 2 0.0 3 0.0 4 0.0 5 0.03593196546175926 6 0.046485409863115126 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 8.375749524885608E-5 21 0.0 22 0.0 23 0.0 24 0.002177694876470258 25 0.008710779505881032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1193923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.35205637125509 #Duplication Level Percentage of deduplicated Percentage of total 1 88.42150971866727 70.16428623627165 2 8.39699225181473 13.32637205029989 3 1.7792154920876397 4.2355322407424625 4 0.584707826883323 1.85591073758238 5 0.26184115552832254 1.0388817066899008 6 0.12969347506805665 0.6174866366750638 7 0.0873850169160821 0.4853926551829617 8 0.06133335770938241 0.38935424467146124 9 0.03903372569158796 0.2787665761313096 >10 0.20886960017060768 2.8680969965740553 >50 0.014992020626314936 0.8317052893971454 >100 0.01210973219972699 1.915841556048832 >500 0.0014742169508491615 0.796648418259121 >1k 8.424096861995208E-4 1.1957246554738854 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3246 0.2718768295777868 No Hit TATCAACGCAGAGTACTTTTTTTTT 2320 0.19431738897734613 No Hit GGTATCAACGCAGAGTACTTTTTTT 2232 0.1869467293954468 No Hit CTTTAGGACGTGAAATATGGCGAGG 1437 0.12035952067260619 No Hit CTGTAGGACGTGGAATATGGCAAGA 1355 0.1134914060622 No Hit GTCCTACAGTGGACATTTCTAAATT 1324 0.11089492370948545 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1318 0.11039237873799232 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.375749524885608E-5 0.0 2 0.0 0.0 0.0 8.375749524885608E-5 0.0 3 0.0 0.0 0.0 8.375749524885608E-5 0.0 4 0.0 0.0 0.0 8.375749524885608E-5 0.0 5 0.0 0.0 0.0 8.375749524885608E-5 0.0 6 0.0 0.0 0.0 8.375749524885608E-5 0.0 7 0.0 0.0 0.0 1.6751499049771215E-4 0.0 8 0.0 0.0 0.0 1.6751499049771215E-4 0.0 9 0.0 0.0 0.0 1.6751499049771215E-4 0.0 10 0.0 0.0 0.0 1.6751499049771215E-4 0.0 11 0.0 0.0 0.0 1.6751499049771215E-4 0.0 12 0.0 0.0 0.0 1.6751499049771215E-4 0.0 13 0.0 0.0 0.0 1.6751499049771215E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACACTCG 40 0.0052922997 14.244252 8 TAGGGTG 100 1.9219715E-6 11.403044 5 GGTATCA 955 0.0 11.288781 1 ATTTAGA 315 0.0 10.90345 1 TTATGCT 140 8.303687E-9 10.860041 4 TAGGACC 335 0.0 10.7789955 4 TGGACTG 155 3.6397978E-9 10.422135 5 GTAACAC 140 9.636824E-8 10.181289 3 CCCATAG 75 0.0025549582 10.176554 1 CTTAAGC 75 0.0026409475 10.136038 3 TCTTATA 170 1.54796E-9 10.061508 2 CGAAATC 85 6.615048E-4 10.054766 13 ACGAAAT 85 6.615048E-4 10.054766 12 GGCGAGG 450 0.0 9.9182205 19 AACAGTA 135 6.470018E-7 9.8478775 7 TAGCACT 175 2.5556801E-9 9.774037 4 GACGTGA 525 0.0 9.767487 7 TTTAGAA 450 0.0 9.713703 2 AGGACGT 900 0.0 9.713703 5 TTAGGAC 690 0.0 9.640253 3 >>END_MODULE