FastQCFastQC Report
Thu 26 May 2016
SRR1511970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602487
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15610.2590927273119586No Hit
GTCCTAAAGTGTGTATTTCTCATTT10440.17328174715802996No Hit
GTCCTACAGTGGACATTTCTAAATT10190.16913228003259823No Hit
CTGTAGGACGTGGAATATGGCAAGA9870.16382096211204558No Hit
TATCAACGCAGAGTACTTTTTTTTT9810.16282509000194195No Hit
GGTATCAACGCAGAGTACTTTTTTT9290.15419419838104392No Hit
CTTTAGGACGTGAAATATGGCGAGG8480.14074992489464502No Hit
GTCCTACAGTGTGCATTTCTCATTT6900.11452529266191636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCC400.00538612914.2049343
ATCTAGA603.2432436E-413.0177131
CGGTAGG450.00959512413.0177121
GTAGCCC751.5238478E-512.6266083
CTTGGAC905.597467E-712.6266073
GTACAAA550.002496695112.4259981
CCTCTAA550.002496695112.4259981
TCCTATA1051.8989886E-712.0878752
GTGTAGC1051.8989886E-712.0878751
AGACTAT550.003138259812.0526726
AACCATA550.003138259812.0526727
CCAGAAC550.003138259812.0526723
CTTATTC550.003138259812.0526723
TTGGACT951.0766598E-611.962054
GGTATCA3400.011.7733721
GTATTAG751.6051266E-411.715941
TAGAAAT2250.011.3639474
GTCCTAT951.0012689E-511.3048551
CTAGACA855.4841847E-511.1411254
TCTAGAA1156.171322E-711.0367562
Produced by FastQC (version 0.11.5)