##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511970_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602487 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.43419692043148 31.0 31.0 34.0 25.0 34.0 2 27.62796541668119 31.0 27.0 34.0 10.0 34.0 3 29.533455493645505 31.0 30.0 34.0 16.0 34.0 4 34.00816947087655 37.0 35.0 37.0 30.0 37.0 5 34.44339711894199 37.0 35.0 37.0 32.0 37.0 6 34.730631532298624 37.0 35.0 37.0 32.0 37.0 7 34.81900024398867 37.0 35.0 37.0 32.0 37.0 8 34.85840856317232 37.0 35.0 37.0 33.0 37.0 9 36.60005776058239 39.0 37.0 39.0 33.0 39.0 10 36.53480158078099 39.0 37.0 39.0 33.0 39.0 11 36.551714808784254 39.0 37.0 39.0 33.0 39.0 12 36.476911535020676 39.0 37.0 39.0 33.0 39.0 13 36.47749577999193 39.0 37.0 39.0 33.0 39.0 14 37.828296710136485 40.0 38.0 41.0 33.0 41.0 15 37.791864388775195 40.0 38.0 41.0 33.0 41.0 16 37.75626030105214 40.0 38.0 41.0 33.0 41.0 17 37.729697072301974 40.0 38.0 41.0 33.0 41.0 18 37.69811132854319 40.0 38.0 41.0 33.0 41.0 19 37.72212014533093 40.0 38.0 41.0 33.0 41.0 20 37.66208731474704 40.0 38.0 41.0 33.0 41.0 21 37.60902392914702 40.0 38.0 41.0 33.0 41.0 22 37.5329027846244 40.0 38.0 41.0 33.0 41.0 23 37.46577436525602 40.0 38.0 41.0 33.0 41.0 24 37.40672246870057 40.0 38.0 41.0 33.0 41.0 25 37.324607833861975 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2951025007985955 1101 2 4.384481362831892 1101 3 2.4422386942701877 1101 4 1.516083489147583 1101 5 1.1873671875857568 1101 6 0.7452277108505712 1101 7 0.47161063229142997 1101 8 0.6230348802573857 1101 9 0.5321450298824146 1101 10 0.732825527360589 1101 11 0.7138448699576259 1101 12 0.7779419932026173 1101 13 0.7451282377568376 1101 14 0.5022741661751411 1101 15 0.833071116104783 1101 16 0.8854105766171187 1101 17 0.8912202722928981 1101 18 1.037423314065684 1101 19 0.6154004454870474 1101 20 0.8950386474762482 1101 21 0.535868666493613 1101 22 0.6841670873054824 1101 23 0.719941692401342 1101 24 0.7088074216396834 1101 25 0.44479796628742463 1102 1 1.3984780282247584 1102 2 4.174975971364475 1102 3 2.382229317805116 1102 4 1.3968851769112973 1102 5 0.9334798614410502 1102 6 0.7189345400426319 1102 7 0.45471346085839315 1102 8 0.5503282073397386 1102 9 0.6333131811560548 1102 10 0.38943202837637614 1102 11 0.5260420882729875 1102 12 0.4767621213476403 1102 13 0.4677606803259877 1102 14 0.5542744787239755 1102 15 0.5997924788177045 1102 16 0.6112271104505282 1102 17 0.5762687173624457 1102 18 0.6551565536343702 1102 19 0.5315592202956125 1102 20 0.5357442264729642 1102 21 0.44741734597478455 1102 22 0.45166982210778883 1102 23 0.6241064056377823 1102 24 0.8153865746323419 1102 25 0.6647542094504217 1103 1 1.4139653273434725 1103 2 4.259835714466831 1103 3 2.5958402849153046 1103 4 1.6669733054753166 1103 5 1.189631100808981 1103 6 1.0985113693343393 1103 7 0.8375279108708753 1103 8 0.7056308012763424 1103 9 0.798098235780401 1103 10 0.70884527262114 1103 11 0.6305771572974308 1103 12 0.8336564054710536 1103 13 0.8961706221496115 1103 14 1.0357751603283347 1103 15 1.0347808627433608 1103 16 0.9248371175545742 1103 17 0.9421558590401489 1103 18 0.997979547701533 1103 19 0.8129490211903416 1103 20 0.8944505221932602 1103 21 0.759966546289661 1103 22 0.9223824346685134 1103 23 0.9929671663466522 1103 24 0.9768572080443931 1103 25 1.2101408999252428 1104 1 1.4937257395871697 1104 2 4.12275614248875 1104 3 2.326075425625376 1104 4 1.5611285802500134 1104 5 1.141703499726617 1104 6 0.8497807874747707 1104 7 0.769201683599178 1104 8 0.7503047724886258 1104 9 0.8345238155923198 1104 10 0.7406755713269746 1104 11 0.6540761535714594 1104 12 0.8030974092472789 1104 13 0.8052599324052068 1104 14 0.7179394899926521 1104 15 0.7837111848150329 1104 16 0.8544858511513809 1104 17 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-1.0128658521298135 2315 14 -1.270197496154502 2315 15 -1.1944175267744157 2315 16 -0.8427131829206616 2315 17 -0.9712427894986604 2315 18 -0.9855390199880318 2315 19 -1.047155237483885 2315 20 -1.031167633121818 2315 21 -1.0185352500591165 2315 22 -1.5133310382182685 2315 23 -1.3852550173415281 2315 24 -1.2992142340741637 2315 25 -1.373019481778556 2316 1 -0.5935562855007319 2316 2 -0.01776640336319346 2316 3 -0.12968517021231207 2316 4 -0.5173305093632123 2316 5 -0.6685989330397035 2316 6 -0.9703810009885956 2316 7 -0.9572101048641883 2316 8 -1.0568794905542305 2316 9 -1.0782654317706033 2316 10 -1.0312288285589943 2316 11 -1.1311383765055183 2316 12 -1.0963134051592647 2316 13 -1.2768607793690023 2316 14 -1.2887719991264248 2316 15 -1.324272517551215 2316 16 -1.5278433325414866 2316 17 -1.6290235847435852 2316 18 -1.528939306932081 2316 19 -1.2783309394719993 2316 20 -1.37145893256843 2316 21 -1.4214098145190448 2316 22 -1.327560388494966 2316 23 -1.2974168332874143 2316 24 -1.570325117451901 2316 25 -1.41535653855248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14531.0 3 0.0 4 106.0 5 579.0 6 696.0 7 553.0 8 575.0 9 522.0 10 467.0 11 469.0 12 466.0 13 488.0 14 433.0 15 484.0 16 492.0 17 483.0 18 598.0 19 743.0 20 880.0 21 1198.0 22 1299.0 23 1651.0 24 1916.0 25 2042.0 26 2425.0 27 2664.0 28 3258.0 29 3981.0 30 5182.0 31 7217.0 32 9637.0 33 14499.0 34 22229.0 35 36493.0 36 67612.0 37 102235.0 38 293384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.23904250216187 19.255851163593572 13.40344272988463 27.101663604359928 2 15.91944866110011 21.362499850254054 40.38879181548561 22.32925967316022 3 17.11754776451608 24.64567700215938 30.930459910338314 27.306315322986226 4 11.410204701512232 17.148751757299326 39.44914994016469 31.991893601023758 5 12.324415298587356 37.462551059192975 37.073497021512495 13.139536620707167 6 29.621552000292123 38.19567891091426 19.07244471664949 13.110324372144131 7 26.05334222979085 31.783756330012096 23.85478856805209 18.30811287214496 8 23.14157815853288 37.48031077848983 21.92379254656117 17.454318516416123 9 24.929500553538915 15.295931696451543 21.362784591202796 38.41178315880674 10 13.791998831510059 28.74651237288107 35.84990215556518 21.611586640043686 11 32.10940651001599 22.569615609963368 26.45833022123299 18.862647658787658 12 21.848770180933364 24.5631855957058 33.35159098868523 20.236453234675604 13 27.34349454843009 22.37741229271337 27.013860879985792 23.265232278870748 14 19.463656477235194 22.392350374364923 28.94917234728716 29.19482080111272 15 21.325273408388895 31.519850220834638 24.618124540446516 22.536751830329948 16 20.63546599345712 28.202932179449512 29.291420395792773 21.87018143130059 17 20.14001961868057 28.8909138288461 29.308848157719584 21.66021839475375 18 20.038307944085005 27.531760074093008 31.40720282297016 21.022729158851824 19 21.849838585192995 27.074532975924715 28.953915865125275 22.121712573757023 20 21.878055055312583 27.486825398142695 29.69451521614646 20.940604330398266 21 21.135868699208586 26.957627343708502 28.742630265044884 23.16387369203803 22 21.491549842814795 28.233915488469215 28.905147774011823 21.36938689470417 23 20.411066222725328 28.216444640669764 29.373989596369682 21.99849954023523 24 21.551870084948032 26.84161955630504 30.065428906231844 21.54108145251508 25 21.059113504900537 27.535955251997972 29.553100907076523 21.851830336024964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 447.0 1 447.0 2 368.5 3 290.0 4 290.0 5 290.0 6 598.5 7 907.0 8 907.0 9 907.0 10 1287.0 11 1667.0 12 1667.0 13 1667.0 14 2624.0 15 3581.0 16 3581.0 17 3581.0 18 6386.0 19 9191.0 20 9191.0 21 9191.0 22 14087.0 23 18983.0 24 18983.0 25 18983.0 26 26679.0 27 34375.0 28 34375.0 29 34375.0 30 42960.0 31 51545.0 32 51545.0 33 51545.0 34 57599.5 35 63654.0 36 63654.0 37 63654.0 38 67660.0 39 71666.0 40 71666.0 41 71666.0 42 73527.5 43 75389.0 44 75389.0 45 75389.0 46 75494.0 47 75599.0 48 75599.0 49 75599.0 50 71593.0 51 67587.0 52 67587.0 53 67587.0 54 59319.5 55 51052.0 56 51052.0 57 51052.0 58 43063.0 59 35074.0 60 35074.0 61 35074.0 62 28381.5 63 21689.0 64 21689.0 65 21689.0 66 16622.5 67 11556.0 68 11556.0 69 11556.0 70 8384.0 71 5212.0 72 5212.0 73 5212.0 74 3595.0 75 1978.0 76 1978.0 77 1978.0 78 1342.0 79 706.0 80 706.0 81 706.0 82 471.5 83 237.0 84 237.0 85 237.0 86 154.0 87 71.0 88 71.0 89 71.0 90 46.0 91 21.0 92 21.0 93 21.0 94 15.5 95 10.0 96 10.0 97 10.0 98 5.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.0148368346536936 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.6597868501727007E-4 19 3.3195737003454014E-4 20 3.3195737003454014E-4 21 0.0038175097553972115 22 0.002157722905224511 23 8.298934250863504E-4 24 1.6597868501727007E-4 25 3.3195737003454014E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 602487.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.45061430089535 #Duplication Level Percentage of deduplicated Percentage of total 1 89.48207700324346 72.88370140834202 2 7.812816682838304 12.727174364749269 3 1.5391065079990185 3.7608351165307785 4 0.4780001420571124 1.557336208258682 5 0.23207288438346724 0.9451239497807036 6 0.10875883552731141 0.5315084378609733 7 0.07059177693222955 0.40248205170053103 8 0.04567188536997709 0.29760024957317677 9 0.03094286576825579 0.22682838825491222 >10 0.17105431391288048 2.519290095458234 >50 0.015279501049368592 0.8745300453515631 >100 0.011975770504311714 1.9182411475835615 >500 0.0010323940089407785 0.7458705496493714 >1k 6.19436405364467E-4 0.6094779869062732 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1561 0.2590927273119586 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1044 0.17328174715802996 No Hit GTCCTACAGTGGACATTTCTAAATT 1019 0.16913228003259823 No Hit CTGTAGGACGTGGAATATGGCAAGA 987 0.16382096211204558 No Hit TATCAACGCAGAGTACTTTTTTTTT 981 0.16282509000194195 No Hit GGTATCAACGCAGAGTACTTTTTTT 929 0.15419419838104392 No Hit CTTTAGGACGTGAAATATGGCGAGG 848 0.14074992489464502 No Hit GTCCTACAGTGTGCATTTCTCATTT 690 0.11452529266191636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTACCC 40 0.005386129 14.204934 3 ATCTAGA 60 3.2432436E-4 13.017713 1 CGGTAGG 45 0.009595124 13.017712 1 GTAGCCC 75 1.5238478E-5 12.626608 3 CTTGGAC 90 5.597467E-7 12.626607 3 GTACAAA 55 0.0024966951 12.425998 1 CCTCTAA 55 0.0024966951 12.425998 1 TCCTATA 105 1.8989886E-7 12.087875 2 GTGTAGC 105 1.8989886E-7 12.087875 1 AGACTAT 55 0.0031382598 12.052672 6 AACCATA 55 0.0031382598 12.052672 7 CCAGAAC 55 0.0031382598 12.052672 3 CTTATTC 55 0.0031382598 12.052672 3 TTGGACT 95 1.0766598E-6 11.96205 4 GGTATCA 340 0.0 11.773372 1 GTATTAG 75 1.6051266E-4 11.71594 1 TAGAAAT 225 0.0 11.363947 4 GTCCTAT 95 1.0012689E-5 11.304855 1 CTAGACA 85 5.4841847E-5 11.141125 4 TCTAGAA 115 6.171322E-7 11.036756 2 >>END_MODULE