##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511970_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 602487 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.574946845326124 31.0 28.0 31.0 23.0 34.0 2 28.7659783530599 31.0 28.0 33.0 23.0 34.0 3 28.754481009548755 31.0 28.0 33.0 23.0 34.0 4 32.24079191750195 35.0 32.0 37.0 25.0 37.0 5 31.725469595194586 35.0 32.0 37.0 22.0 37.0 6 31.748993754222084 35.0 32.0 37.0 19.0 37.0 7 31.494110246362162 35.0 32.0 37.0 18.0 37.0 8 31.341642226305297 35.0 32.0 37.0 17.0 37.0 9 32.444080951124256 37.0 32.0 39.0 17.0 39.0 10 32.06913510167024 37.0 32.0 39.0 16.0 39.0 11 32.16929327935706 37.0 32.0 39.0 15.0 39.0 12 31.845603307623236 37.0 31.0 39.0 11.0 39.0 13 31.849560239474048 37.0 32.0 39.0 10.0 39.0 14 32.32789753139902 37.0 31.0 40.0 10.0 41.0 15 32.239100594701625 37.0 31.0 40.0 10.0 41.0 16 32.055058449393925 37.0 31.0 40.0 10.0 41.0 17 31.93913063684362 37.0 31.0 40.0 8.0 41.0 18 31.767405769751047 37.0 31.0 40.0 2.0 41.0 19 31.798704370384755 37.0 31.0 40.0 2.0 41.0 20 31.602379802385776 37.0 30.0 40.0 2.0 41.0 21 31.383143536707017 37.0 30.0 40.0 2.0 41.0 22 31.16702269094603 37.0 30.0 39.0 2.0 41.0 23 31.17821297388989 37.0 30.0 40.0 2.0 41.0 24 30.91626541319564 37.0 30.0 40.0 2.0 41.0 25 30.263504440759718 36.0 29.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08484790216215998 1101 2 -0.037257858951033285 1101 3 -0.01993148628148944 1101 4 -0.23000248421898206 1101 5 0.07338861751408743 1101 6 0.258557372693776 1101 7 0.22935396382627715 1101 8 0.23317055467217784 1101 9 0.0865775207368813 1101 10 0.06446601579435907 1101 11 -0.07526303170210724 1101 12 0.029768999558612563 1101 13 -0.1491478688941683 1101 14 -0.47983004635839066 1101 15 -0.06114632752843363 1101 16 -0.044854481627531584 1101 17 -0.41366100353337387 1101 18 -0.23866130113631456 1101 19 0.012075816139137885 1101 20 -0.05634366021585535 1101 21 -0.3501133417429827 1101 22 -0.40280624707437696 1101 23 -0.6499997276957679 1101 24 -0.825432547304402 1101 25 -0.9813753466805188 1102 1 -0.14782649256691016 1102 2 -0.11914565391899856 1102 3 -0.18966503839900994 1102 4 -0.2537952643408765 1102 5 -0.16619959939480466 1102 6 -0.09868594657486085 1102 7 -0.12197046186523863 1102 8 -0.10240922341814951 1102 9 -0.29566970539219994 1102 10 -0.45500968353366034 1102 11 -0.47569669321172015 1102 12 0.037660857661443004 1102 13 0.11458474557684539 1102 14 0.3952285565483109 1102 15 0.22581257229966667 1102 16 0.16279515112758958 1102 17 0.3291699075464791 1102 18 0.20783424504277903 1102 19 0.1383064771799276 1102 20 0.0871357896981948 1102 21 0.20833329054331173 1102 22 0.06802903968917917 1102 23 0.36554957688307255 1102 24 -0.25171009067055294 1102 25 0.07103518621524785 1103 1 0.41020347882490427 1103 2 0.1717530078324394 1103 3 0.4146660293198252 1103 4 0.6182095164897206 1103 5 0.20336676582993363 1103 6 0.35707697030780494 1103 7 0.8664806642219709 1103 8 0.4854049385924881 1103 9 0.3831698391808871 1103 10 0.26024529409819763 1103 11 0.10929265072007865 1103 12 0.6023052713856885 1103 13 0.5414514775239248 1103 14 0.3461242422807018 1103 15 0.33635007973970943 1103 16 0.585601057085384 1103 17 0.5206215328368842 1103 18 0.23369681056319536 1103 19 0.2898530470779761 1103 20 0.2741113863420104 1103 21 0.3402645349032767 1103 22 0.4398370192602492 1103 23 0.3461934530038775 1103 24 0.43870952801500707 1103 25 0.6234635217565945 1104 1 -0.22453574085666617 1104 2 -0.23275232736681062 1104 3 -0.17675343266295584 1104 4 0.1537243311835681 1104 5 0.14439943528877563 1104 6 0.09472636719286598 1104 7 -0.05875619752011474 1104 8 0.10695125445336728 1104 9 0.17907051679013364 1104 10 0.07305479720987051 1104 11 0.3130904784510591 1104 12 0.2938561347910209 1104 13 0.4230771367294359 1104 14 0.2932420789560197 1104 15 0.503742427302754 1104 16 0.684624465633263 1104 17 0.8290078580441005 1104 18 0.32714256790522 1104 19 0.7809200115543646 1104 20 0.33706598375870556 1104 21 0.4998104839456552 1104 22 0.4625697166073479 1104 23 0.6043432305259451 1104 24 0.7289860739916882 1104 25 0.741513755791825 1105 1 -0.45355773663148824 1105 2 -0.2856366320766419 1105 3 -0.37279419319483154 1105 4 -0.3474562330018429 1105 5 -0.6662803211421924 1105 6 -0.5159509038398191 1105 7 -0.7537083536031304 1105 8 -0.49223353490912913 1105 9 -0.6436659067217363 1105 10 -0.4044921146827605 1105 11 -0.329941707456733 1105 12 -0.24637997804606115 1105 13 -0.557400059741294 1105 14 -0.2022643209445647 1105 15 -0.13392626910584227 1105 16 -0.10389050889140705 1105 17 -0.7068937201838139 1105 18 -0.3842455270369953 1105 19 -0.5951977963243458 1105 20 -0.34254132331225406 1105 21 -0.2011016572249673 1105 22 -0.33109251776571114 1105 23 -0.7144836614835022 1105 24 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-0.8399515425703221 2315 5 -1.0373819024876774 2315 6 -0.8596228416580978 2315 7 -0.7677957296417226 2315 8 -0.5322442558954599 2315 9 -1.0895570887104782 2315 10 -1.0611900508928898 2315 11 -1.16107545176121 2315 12 -1.0282239876949468 2315 13 -0.8745536378704841 2315 14 -1.000758022365094 2315 15 -1.100460710274433 2315 16 -0.81143808326825 2315 17 -1.008233843475157 2315 18 -0.7860359317542276 2315 19 -0.7366451294789975 2315 20 -0.6572290745853699 2315 21 -0.6210909362386374 2315 22 -1.00884647112942 2315 23 -0.7375873870262488 2315 24 -0.925038907292052 2315 25 -0.7670675797330482 2316 1 -1.0729808135545653 2316 2 -1.1220959080947424 2316 3 -1.055905052923336 2316 4 -1.5667718438615736 2316 5 -1.2200076967078033 2316 6 -1.228179410627149 2316 7 -1.3375205707977003 2316 8 -1.4681045756331095 2316 9 -1.4311998433733173 2316 10 -1.6287115716963356 2316 11 -1.44907632284135 2316 12 -1.5182578061003582 2316 13 -1.271285806347631 2316 14 -1.7587066286368724 2316 15 -1.2701932374316378 2316 16 -1.357470825633488 2316 17 -1.1470238106815493 2316 18 -1.8063269750007969 2316 19 -1.8627147646501392 2316 20 -1.3869700563439267 2316 21 -1.9793482635598103 2316 22 -1.5178006625620917 2316 23 -1.6121646569586154 2316 24 -1.7036436418688083 2316 25 -1.3550722938138016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28190.0 3 2.0 4 392.0 5 3097.0 6 3864.0 7 3240.0 8 2690.0 9 2417.0 10 2286.0 11 2099.0 12 2038.0 13 1989.0 14 2034.0 15 1957.0 16 2062.0 17 2037.0 18 2261.0 19 2500.0 20 3103.0 21 3896.0 22 4883.0 23 6009.0 24 6946.0 25 8091.0 26 9346.0 27 10866.0 28 12480.0 29 15108.0 30 18735.0 31 23769.0 32 29619.0 33 37531.0 34 47647.0 35 60040.0 36 76699.0 37 91496.0 38 71068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.67162873467266 23.752109878647612 15.633013581536709 19.94324780514302 2 17.682705186999886 25.217141614673842 38.30954028883611 18.79061290949016 3 20.95115745235997 28.310652346025723 31.01228740205183 19.72590279956248 4 16.17412491887792 21.22087281551303 37.271841550108135 25.333160715500913 5 13.233763106989565 40.2757967970378 35.49061444072694 10.9998256552457 6 30.293228300956283 40.61508368107043 17.830485846699002 11.261202171274288 7 26.986640375642963 33.69018750612046 23.334113433152915 15.989058685083663 8 25.005518791276828 39.06158971064936 20.800448806364287 15.13244269170953 9 27.24506918821485 15.92416101924191 21.098878482025338 35.7318913105179 10 15.323650136849427 31.272707958843927 34.37318979496653 19.030452109340118 11 34.35543007566969 23.52764457988305 25.19124893981115 16.925676404636118 12 23.20597788831958 26.70381269637353 32.18658659854901 17.90362281675787 13 29.598149005704688 23.392537930278994 26.177494286183766 20.83181877783255 14 21.138215430374434 23.92167797811405 28.505013386180945 26.435093205330574 15 23.17510585290637 33.023617107091106 23.7606786536473 20.040598386355224 16 22.12446077674705 29.922969292283486 28.092888311283065 19.859681619686402 17 21.789183833012167 30.391693098772254 28.094548098133238 19.724574970082344 18 21.47747586254973 28.729416568324297 30.713193811650708 19.079913757475264 19 23.853294759886936 28.08956873758272 28.58634294183941 19.470793560690936 20 23.808978450987325 28.567089414377406 28.8947313386015 18.729200796033773 21 23.209961376759995 27.86516555543937 28.178533312751973 20.746339755048655 22 23.422746050952135 29.10170675881803 28.429161127128054 19.046386063101778 23 22.485796374029647 29.11946647811488 28.709333147437206 19.685404000418266 24 23.109749316566884 28.33521170243314 29.224214197150744 19.330824783849234 25 22.894316176678082 28.707283347913197 28.859168466325492 19.53923200908323 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 531.0 1 531.0 2 436.5 3 342.0 4 342.0 5 342.0 6 759.0 7 1176.0 8 1176.0 9 1176.0 10 1637.5 11 2099.0 12 2099.0 13 2099.0 14 3291.5 15 4484.0 16 4484.0 17 4484.0 18 7677.0 19 10870.0 20 10870.0 21 10870.0 22 16597.0 23 22324.0 24 22324.0 25 22324.0 26 30836.5 27 39349.0 28 39349.0 29 39349.0 30 48077.5 31 56806.0 32 56806.0 33 56806.0 34 62181.0 35 67556.0 36 67556.0 37 67556.0 38 70172.5 39 72789.0 40 72789.0 41 72789.0 42 73959.5 43 75130.0 44 75130.0 45 75130.0 46 73538.0 47 71946.0 48 71946.0 49 71946.0 50 67489.5 51 63033.0 52 63033.0 53 63033.0 54 54650.5 55 46268.0 56 46268.0 57 46268.0 58 38710.5 59 31153.0 60 31153.0 61 31153.0 62 25117.5 63 19082.0 64 19082.0 65 19082.0 66 14662.5 67 10243.0 68 10243.0 69 10243.0 70 7381.0 71 4519.0 72 4519.0 73 4519.0 74 3178.5 75 1838.0 76 1838.0 77 1838.0 78 1247.5 79 657.0 80 657.0 81 657.0 82 438.0 83 219.0 84 219.0 85 219.0 86 139.5 87 60.0 88 60.0 89 60.0 90 35.5 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3873942508303084 2 0.0 3 0.0 4 0.0 5 0.038507054924006655 6 0.04315445810449022 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0023237015902417813 25 0.009626763731001664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 602487.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.76929965772248 #Duplication Level Percentage of deduplicated Percentage of total 1 90.76406216463621 74.2171379729229 2 6.7479453853405245 11.0354953657571 3 1.365884572908742 3.350622748201056 4 0.4684964971236263 1.5323452184758046 5 0.20109859385267492 0.8221845590743003 6 0.10936818996128057 0.5365776178780001 7 0.07206482560884134 0.41248832239936023 8 0.045558053471206714 0.2980200100887716 9 0.030999141278069787 0.22813002650687061 >10 0.16241102595020276 2.45845370297588 >50 0.017884965749731835 1.0188878771754233 >100 0.012194214959090975 2.2217326506154906 >500 0.001422658411841618 0.8952407651025956 >1k 6.09710747932122E-4 0.9726831628263398 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2317 0.3845726131850148 No Hit TATCAACGCAGAGTACTTTTTTTTT 1783 0.2959399953857926 No Hit GGTATCAACGCAGAGTACTTTTTTT 1753 0.29096063483527446 No Hit CTGTAGGACGTGGAATATGGCAAGA 943 0.1565178999712857 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 805 0.13361284143890242 No Hit CTTTAGGACGTGAAATATGGCGAGG 788 0.13079120379360884 No Hit GTCCTAAAGTGTGTATTTCTCATTT 771 0.12796956614831523 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 740 0.12282422691277986 No Hit GTCCTACAGTGGACATTTCTAAATT 731 0.12133041874762443 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 609 0.10108101917551748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 40 0.005261915 14.254983 3 AACCTCA 40 0.005267718 14.252616 6 TAGGACT 75 9.606465E-7 13.938206 4 GGTATCA 605 0.0 12.921317 1 CACCTTT 165 0.0 12.661631 14 GGCGAGG 265 0.0 12.543223 19 CCACCTT 170 0.0 12.289231 13 TAGGCTG 70 1.0852131E-4 12.218557 5 TCATACA 70 1.0852131E-4 12.218557 2 ATAGAAC 105 2.6967427E-7 11.766018 3 TTTATCC 105 2.6967427E-7 11.766018 3 ATATACT 85 5.291199E-5 11.18038 4 GACGTGG 260 0.0 10.957181 7 GTGTAGG 70 0.001446108 10.895328 1 GTAGACC 70 0.001484767 10.86094 3 CCTGGAC 70 0.001484767 10.86094 3 GACAGCC 70 0.0014940538 10.852827 7 TAGGACC 255 0.0 10.8077 4 ACCTTTT 195 1.8189894E-12 10.713688 15 AATGTCC 170 1.382432E-10 10.613427 8 >>END_MODULE