FastQCFastQC Report
Thu 26 May 2016
SRR1511969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117255
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28520.25526849286868264No Hit
GTCCTACAGTGGACATTTCTAAATT22520.20156544387807618No Hit
GTCCTAAAGTGTGTATTTCTCATTT21090.18876621720198164No Hit
CTGTAGGACGTGGAATATGGCAAGA20600.18438046820108211No Hit
CTTTAGGACGTGAAATATGGCGAGG18340.1641523197479537No Hit
GGTATCAACGCAGAGTACTTTTTTT17900.1602140961553092No Hit
TATCAACGCAGAGTACTTTTTTTTT16590.1484889304590268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG357.944034E-519.561791
TAGGACC3500.013.2550754
GGCGTGC500.001538312913.2544818
AAGGCGT751.530002E-512.6238816
GGTATCA6400.012.226121
TAGTACC550.0031462312.0500694
CGTGCAC550.003147284712.0495310
GTCCTAA6650.011.61941051
GGCGAGG6650.011.39098719
ATTATAC855.50601E-511.138723
GACTTAA907.073627E-510.8676621
AAAGGCG1053.6059864E-610.820475
TTAGGAC8950.010.7902453
AGGACCT8450.010.7564445
ACTGTTC2750.010.6724418
GGTCTAT1104.263273E-610.6700691
TGGCGAG14050.010.44596218
GTCTAGA1501.5510523E-810.4329561
TCCTAAA7750.010.3488192
GGACCTG8600.010.3486466
Produced by FastQC (version 0.11.5)