FastQCFastQC Report
Thu 26 May 2016
SRR1511969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117255
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35310.3160424433097189No Hit
GTCCTACAGTGGACATTTCTAAATT23450.20988941647162015No Hit
TATCAACGCAGAGTACTTTTTTTTT23240.20800980975694897No Hit
GGTATCAACGCAGAGTACTTTTTTT23030.20613020304227772No Hit
GTCCTAAAGTGTGTATTTCTCATTT22340.199954352408358No Hit
CTGTAGGACGTGGAATATGGCAAGA21610.19342048144783422No Hit
CTTTAGGACGTGAAATATGGCGAGG19620.17560897019928307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3900.012.1850294
TCCAACG907.4955824E-611.60547618
TATACTG2100.011.3146715
GGCGAGG4850.011.16053119
AATCCCG1752.0008883E-1110.85284619
TAGAAAT4200.010.635794
GTGCTAG1355.318907E-810.6026291
ACTGTTC3900.010.4692778
GTATAAG1303.6083475E-710.2763941
TTAGGAC6750.010.1379443
GACCCTA750.002657252610.12841611
GGTATCA8400.010.1103631
GGTCTAC951.5707259E-410.0445951
AGGACCT7650.09.939165
GTCCTAA6350.09.918051
CTCTTAG1356.052851E-79.8957861
GAACAGT2600.09.8650126
TGGCGAG10400.09.76929718
TAGGACT2054.0017767E-119.73613554
GTAGAAC4050.09.621663
Produced by FastQC (version 0.11.5)