FastQCFastQC Report
Thu 26 May 2016
SRR1511968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992897
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27310.2750537064771069No Hit
GGTATCAACGCAGAGTACTTTTTTT17510.17635263275042626No Hit
TATCAACGCAGAGTACTTTTTTTTT17380.17504333279282747No Hit
CTGTAGGACGTGGAATATGGCAAGA14200.14301584152233313No Hit
GTCCTACAGTGGACATTTCTAAATT14090.14190797232744182No Hit
GTCCTAAAGTGTGTATTTCTCATTT14060.14160582618338055No Hit
CTTTAGGACGTGAAATATGGCGAGG12990.1308292803785287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG357.962867E-519.555391
ACTATAC751.0037566E-613.8863583
CTAGGTA701.1250435E-412.1731054
GCGCCAC550.00314565612.05014513
GCTAGGT550.00314565612.0501453
ATTGCAC550.00314565612.0501453
GTCTAGG1103.4163168E-711.5554561
TAGGACC3250.011.3615664
GACGTGA4850.011.1273067
TTAGGAC5650.011.0599313
AATGCCG600.006015839511.04763518
GGTTAGG802.8862993E-410.9999061
AGGACGT8650.010.8361765
GGCGAGG3950.010.78792719
GGACGTG8450.010.7565126
GGTATCA6100.010.7394351
CTATACC1159.1936454E-710.7029244
GTCCTAC7000.010.6157831
CAGGACT1803.8198777E-1110.5199684
ACGTGAA5350.010.4413148
Produced by FastQC (version 0.11.5)