FastQCFastQC Report
Thu 26 May 2016
SRR1511968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992897
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34270.34515161189932086No Hit
TATCAACGCAGAGTACTTTTTTTTT23180.2334582539780058No Hit
GGTATCAACGCAGAGTACTTTTTTT22320.2247967311815828No Hit
CTTTAGGACGTGAAATATGGCGAGG14440.14543301067482325No Hit
CTGTAGGACGTGGAATATGGCAAGA13870.1396922339376592No Hit
GTCCTACAGTGGACATTTCTAAATT13310.13405217258184887No Hit
GTCCTAAAGTGTGTATTTCTCATTT12690.127807818937916No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11610.1169305577517104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG250.006020883619.0052915
TAGGACC3250.012.865124
TTAGCAC701.0873037E-412.2176883
TCCAACG658.0443063E-411.68790718
GGTATCA8850.011.6410341
AATGTCC2750.011.0498288
CTTAGAC1158.7709304E-710.7421213
TTAGACC803.7584023E-410.6904764
AAATGTC2950.010.6225837
GGCGAGG3850.010.36182319
CTAGGAC1201.5157111E-610.2945333
ATGTCCA2900.010.1508399
ATTGGAC750.00264002410.1361553
TAGGCCT750.00264002410.1361554
TAGGACT1502.4278052E-810.1361554
TAGTACC750.00264002410.1361554
TACTGTC856.614378E-410.0545317
TGTAGGA9350.09.9599922
GAAATGT3500.09.77168756
TTAGGAC6700.09.6444773
Produced by FastQC (version 0.11.5)