##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511968_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992897 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.95784759144201 31.0 30.0 34.0 26.0 34.0 2 30.170686385395463 31.0 30.0 34.0 26.0 34.0 3 30.23365364181783 31.0 30.0 34.0 26.0 34.0 4 33.842717824708906 35.0 35.0 37.0 30.0 37.0 5 33.41870103344053 35.0 35.0 37.0 28.0 37.0 6 33.36901914297253 36.0 35.0 37.0 28.0 37.0 7 33.17092105223402 35.0 35.0 37.0 27.0 37.0 8 33.0448022302414 35.0 33.0 37.0 26.0 37.0 9 34.39140817224747 38.0 35.0 39.0 27.0 39.0 10 34.11481352043565 37.0 34.0 39.0 27.0 39.0 11 34.146671809865474 37.0 34.0 39.0 27.0 39.0 12 33.9175382743628 37.0 34.0 39.0 25.0 39.0 13 33.893416940528574 37.0 34.0 39.0 25.0 39.0 14 34.684163614151316 38.0 34.0 40.0 25.0 41.0 15 34.59032306472877 38.0 34.0 40.0 25.0 41.0 16 34.45434521405544 38.0 33.0 40.0 24.0 41.0 17 34.34648306924082 38.0 33.0 40.0 23.0 41.0 18 34.230650309145865 38.0 33.0 40.0 22.0 41.0 19 34.247035694538305 38.0 34.0 40.0 22.0 41.0 20 34.09468857293355 38.0 33.0 40.0 19.0 41.0 21 33.95039364606802 38.0 33.0 40.0 18.0 41.0 22 33.78596974308513 38.0 33.0 40.0 18.0 41.0 23 33.85566780844337 38.0 34.0 40.0 18.0 41.0 24 33.69570358254683 38.0 33.0 40.0 16.0 41.0 25 33.13223627425604 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49246269824229927 1101 2 0.3571557269774104 1101 3 0.3292871495349772 1101 4 0.42173177266093376 1101 5 0.41783015130593526 1101 6 0.658440484215923 1101 7 0.3623114385095789 1101 8 0.6659511248975392 1101 9 0.3716093523926105 1101 10 0.4170748952387626 1101 11 0.30736075955289976 1101 12 0.5106495768727726 1101 13 0.21533512441455827 1101 14 0.11652538203071572 1101 15 0.21425491358886717 1101 16 0.6143961580637978 1101 17 0.30560133647953336 1101 18 0.6753121910248012 1101 19 0.8692618531172869 1101 20 0.6977402606630392 1101 21 0.41674804169971935 1101 22 0.5809722445895034 1101 23 0.21193485087445652 1101 24 0.056958958355345146 1101 25 0.13983413724927374 1102 1 0.7203657199768614 1102 2 0.6702287372889053 1102 3 0.5575938205045041 1102 4 0.6038749042208735 1102 5 0.7135780037178421 1102 6 0.6418580818882802 1102 7 0.7980548856125722 1102 8 0.6930396767290716 1102 9 0.7269251771842562 1102 10 0.7554599909517847 1102 11 0.7676827568994682 1102 12 0.9752785320461896 1102 13 1.0430465664360824 1102 14 1.0706640673021326 1102 15 1.0999658331202653 1102 16 1.119163624345255 1102 17 1.1201972518361316 1102 18 1.3323097168803812 1102 19 1.1323356317966145 1102 20 1.1982396997545166 1102 21 0.9524315561117334 1102 22 1.232420105709572 1102 23 0.9986830669804689 1102 24 1.2058276647824826 1102 25 1.1868152200332247 1103 1 0.28972707035967105 1103 2 0.21617007890168338 1103 3 0.23505031057774062 1103 4 0.43947920455907763 1103 5 0.567873553684322 1103 6 0.387055483137253 1103 7 0.25348751515950596 1103 8 0.43276941871885555 1103 9 0.416789745764369 1103 10 0.4849277913378671 1103 11 0.7799446682616917 1103 12 0.7468505344856737 1103 13 0.6610412540164106 1103 14 0.7342276222886994 1103 15 0.8087897924353982 1103 16 0.8783235160807621 1103 17 0.4874588355823448 1103 18 0.37336353159084723 1103 19 0.8638265870745059 1103 20 0.7613561848883137 1103 21 0.7319852926983828 1103 22 0.6226006977369138 1103 23 0.9539488643726486 1103 24 0.8530608215391879 1103 25 0.7168465199631626 1104 1 0.31538854783384096 1104 2 0.20866745173589507 1104 3 0.5062346389106516 1104 4 0.4806504319589706 1104 5 0.4099295427250027 1104 6 0.6555657854158596 1104 7 0.5668071958716254 1104 8 0.8180973707658552 1104 9 0.7367386581077611 1104 10 0.8703075887818841 1104 11 0.9160308592401947 1104 12 0.93891180776874 1104 13 0.8440146274169322 1104 14 0.994273166475935 1104 15 1.039166808174464 1104 16 1.354604246941335 1104 17 1.1026812423653212 1104 18 1.2737794911992921 1104 19 1.2388273348446575 1104 20 0.8332787119127261 1104 21 1.0172257057865508 1104 22 1.1181609371031271 1104 23 1.2125995679745074 1104 24 0.8694489521337516 1104 25 1.0356488514107909 1105 1 0.24546484937264168 1105 2 0.12400095407754819 1105 3 0.27109228116832185 1105 4 0.4335390446738572 1105 5 0.3277524232748803 1105 6 0.24021226893087544 1105 7 0.2022657770785301 1105 8 0.3212786665005538 1105 9 0.4154001503241673 1105 10 0.34674045848600343 1105 11 0.4104469132516968 1105 12 0.34457371361077804 1105 13 0.30707042494241676 1105 14 0.5306340996306034 1105 15 0.7842800215118899 1105 16 0.8211558481681038 1105 17 0.334804323628461 1105 18 0.3757763308707567 1105 19 0.569646828239037 1105 20 0.6337634354030399 1105 21 0.7368628207763805 1105 22 0.946937081769164 1105 23 0.6531413603649412 1105 24 1.0198209292833198 1105 25 0.4759704882246112 1106 1 -0.5675537198970382 1106 2 -0.5300282012879514 1106 3 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1107 15 0.5211875144948124 1107 16 0.42520804822764546 1107 17 0.31909772875550146 1107 18 0.5890173490667792 1107 19 0.5308216958091379 1107 20 0.358428498793792 1107 21 0.29111517991144353 1107 22 0.5782875427970708 1107 23 0.7070082383810572 1107 24 0.3603711953638893 1107 25 0.13954360239557673 1108 1 -0.17733571495444878 1108 2 -0.08675771588953296 1108 3 0.048171232278203746 1108 4 0.010983641825248469 1108 5 -0.30221961555724164 1108 6 -0.3249560266773628 1108 7 -0.21345609132534804 1108 8 -0.22183603261613172 1108 9 -0.17501704342448932 1108 10 -0.2597844823653759 1108 11 -0.2813513872050848 1108 12 0.05068980896309938 1108 13 -0.05200091431367326 1108 14 0.09473881365747872 1108 15 -0.030377900758253418 1108 16 -0.24286228466748838 1108 17 -0.06321123546730689 1108 18 -0.08069064615150978 1108 19 -0.16904293403635506 1108 20 0.2977390978858594 1108 21 0.3831631609037558 1108 22 0.1566467522502606 1108 23 0.12453858028845843 1108 24 0.2954726578084532 1108 25 0.257228121040157 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2314 1 -0.21408011226720802 2314 2 -0.314814010167062 2314 3 -0.3912321978338156 2314 4 -0.33196711899733344 2314 5 -0.39600555957066064 2314 6 -0.4340774725699461 2314 7 -0.4741742756077869 2314 8 -0.46016178336523694 2314 9 -0.43724441838511297 2314 10 -0.6058049077227068 2314 11 -0.21705693384713953 2314 12 -0.46447902258631757 2314 13 -0.546524603479007 2314 14 -0.6226725580210157 2314 15 -0.7458954579206818 2314 16 -0.563907802151391 2314 17 -0.5821768537592789 2314 18 -0.6599744399345226 2314 19 -0.48917268047766527 2314 20 -0.5592907269454557 2314 21 -0.6914758421512559 2314 22 -0.6209362715524591 2314 23 -0.7309893800330158 2314 24 -0.7101007247567352 2314 25 -0.6744700115195315 2315 1 -0.5921863712259992 2315 2 -0.5553160574900318 2315 3 -0.9374263823526512 2315 4 -0.7756064014344659 2315 5 -0.6111669626269745 2315 6 -0.6100211006392939 2315 7 -0.6915726897033849 2315 8 -0.8450884030490613 2315 9 -1.0458104700139046 2315 10 -1.2532411286130625 2315 11 -0.7957971060427909 2315 12 -0.6535952256852369 2315 13 -1.0466404720541362 2315 14 -1.0463955274709562 2315 15 -1.1400392358716402 2315 16 -1.2931705976863412 2315 17 -1.0583901565930134 2315 18 -1.1781153473349733 2315 19 -1.1195201553837109 2315 20 -1.2346522088671534 2315 21 -1.1960928311007564 2315 22 -1.2610998705051557 2315 23 -0.9312878350563878 2315 24 -0.9559121692080339 2315 25 -1.0120774001969295 2316 1 -1.3064847207513672 2316 2 -1.4208403375937024 2316 3 -1.311288500938776 2316 4 -1.369922360792721 2316 5 -1.4077952560234905 2316 6 -1.551417723367507 2316 7 -1.3911153508154683 2316 8 -1.668487220049908 2316 9 -1.9165709921871894 2316 10 -2.076152991995741 2316 11 -1.9916806585383782 2316 12 -2.120463497775045 2316 13 -1.963445937065032 2316 14 -2.156223444024995 2316 15 -2.079978972876937 2316 16 -2.277745099602356 2316 17 -1.9669114473977416 2316 18 -2.2751095515931326 2316 19 -2.2153248015174967 2316 20 -2.266471149574091 2316 21 -2.2627766570853964 2316 22 -1.8978006703016277 2316 23 -2.4591038182654295 2316 24 -2.5500830362637323 2316 25 -2.7503686501373004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26523.0 3 3.0 4 450.0 5 3552.0 6 4347.0 7 3513.0 8 3064.0 9 2707.0 10 2578.0 11 2359.0 12 2293.0 13 2203.0 14 2205.0 15 2185.0 16 2246.0 17 2303.0 18 2385.0 19 2710.0 20 3352.0 21 4143.0 22 5309.0 23 6761.0 24 7988.0 25 9453.0 26 10792.0 27 12190.0 28 14255.0 29 17081.0 30 21728.0 31 28205.0 32 36163.0 33 47024.0 34 62655.0 35 85841.0 36 129899.0 37 207287.0 38 215145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.48076854997689 21.494598282333687 14.700905360449978 23.323727807239443 2 15.698002914703135 22.76640980887242 39.487479567367004 22.048107709057437 3 18.992100892640426 26.609003753662265 31.112693461658157 23.286201892039156 4 13.730225793813458 19.205617501110385 38.111506027312 28.952650677764158 5 12.895211170543424 38.77540328390119 35.95626972844392 12.373115817111469 6 30.611105737453855 38.81506827231546 18.16359965178701 12.410226338443682 7 26.964931911366435 32.67186828039565 23.18508364915998 17.17811615907793 8 24.700044415483177 37.61256202808549 21.01637934246956 16.671014213961772 9 26.412205898497028 15.50936300542755 20.83428593298197 37.24414516309345 10 14.914739393914978 29.874095701769672 34.76271959729962 20.448445307015735 11 33.522711822072175 23.132510220093323 25.19647053017584 18.148307427658658 12 22.837514868108173 25.68141509139417 32.1556012355763 19.325468804921357 13 28.565400036458964 22.594992229808327 26.36789113070137 22.47171660303133 14 20.891190123446844 22.78242355450767 28.329121751803054 27.99726457024243 15 22.679996011670898 31.475570980675737 24.07047256664085 21.773960441012512 16 21.715545519827334 29.022547152423666 28.095764213206404 21.166143114542596 17 21.353876585385997 29.283802851655306 28.193357417738195 21.168963145220502 18 21.14690647670403 28.153071265196694 30.28602161150653 20.414000646592747 19 23.04911788433241 27.659767327325994 28.386126657649285 20.904988130692306 20 23.15247518370504 27.972516759056337 28.732918654118862 20.142089403119762 21 22.752007509338835 27.412007489195755 28.03372353829249 21.80226146317292 22 22.881023912852992 28.397507495742257 28.210680463331038 20.510788128073706 23 22.04558982452359 28.238981485491443 28.472540454850805 21.242888235134156 24 22.52691691762265 27.61499491373493 29.046702992335348 20.81138517630707 25 22.4777998033812 28.028050411771343 28.560089606601235 20.934060178246224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 527.0 1 527.0 2 498.5 3 470.0 4 470.0 5 470.0 6 1021.0 7 1572.0 8 1572.0 9 1572.0 10 2234.0 11 2896.0 12 2896.0 13 2896.0 14 4513.0 15 6130.0 16 6130.0 17 6130.0 18 10795.5 19 15461.0 20 15461.0 21 15461.0 22 23602.5 23 31744.0 24 31744.0 25 31744.0 26 43826.0 27 55908.0 28 55908.0 29 55908.0 30 69599.5 31 83291.0 32 83291.0 33 83291.0 34 93142.0 35 102993.0 36 102993.0 37 102993.0 38 109418.5 39 115844.0 40 115844.0 41 115844.0 42 120768.5 43 125693.0 44 125693.0 45 125693.0 46 125295.5 47 124898.0 48 124898.0 49 124898.0 50 118962.0 51 113026.0 52 113026.0 53 113026.0 54 99695.5 55 86365.0 56 86365.0 57 86365.0 58 72882.0 59 59399.0 60 59399.0 61 59399.0 62 47495.0 63 35591.0 64 35591.0 65 35591.0 66 26930.0 67 18269.0 68 18269.0 69 18269.0 70 13317.5 71 8366.0 72 8366.0 73 8366.0 74 5701.5 75 3037.0 76 3037.0 77 3037.0 78 2037.5 79 1038.0 80 1038.0 81 1038.0 82 670.0 83 302.0 84 302.0 85 302.0 86 180.0 87 58.0 88 58.0 89 58.0 90 36.0 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42602606312638674 2 0.0 3 0.0 4 0.0 5 0.036056106524644554 6 0.04038686792285605 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0071538135375573E-4 21 0.0 22 0.0 23 0.0 24 0.002719315296551405 25 0.011380838092974396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 992897.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.6626803976555 #Duplication Level Percentage of deduplicated Percentage of total 1 89.61187473942879 72.28334011941283 2 7.746173032480081 12.496541592477575 3 1.4294982426695089 3.4592147963238236 4 0.47457227798909696 1.531210879400874 5 0.2284704894268807 0.9214521034468206 6 0.11952394779962355 0.5784673200736257 7 0.08560227854853786 0.4833436465110254 8 0.05481494724401878 0.35372164564469083 9 0.03692210903142933 0.26804126523686167 >10 0.1837498094571975 2.6236611180776035 >50 0.013718512490556924 0.7813371738317331 >100 0.012960991627131269 2.1649227464367002 >500 0.0011216233096699405 0.5901864665969732 >1k 9.969984974843916E-4 1.464559126528922 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3427 0.34515161189932086 No Hit TATCAACGCAGAGTACTTTTTTTTT 2318 0.2334582539780058 No Hit GGTATCAACGCAGAGTACTTTTTTT 2232 0.2247967311815828 No Hit CTTTAGGACGTGAAATATGGCGAGG 1444 0.14543301067482325 No Hit CTGTAGGACGTGGAATATGGCAAGA 1387 0.1396922339376592 No Hit GTCCTACAGTGGACATTTCTAAATT 1331 0.13405217258184887 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1269 0.127807818937916 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1161 0.1169305577517104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0071538135375573E-4 0.0 5 0.0 0.0 0.0 1.0071538135375573E-4 0.0 6 0.0 0.0 0.0 1.0071538135375573E-4 0.0 7 0.0 0.0 0.0 1.0071538135375573E-4 0.0 8 0.0 0.0 0.0 2.0143076270751146E-4 0.0 9 0.0 0.0 0.0 3.021461440612672E-4 0.0 10 0.0 0.0 0.0 3.021461440612672E-4 0.0 11 0.0 0.0 0.0 3.021461440612672E-4 0.0 12 0.0 0.0 0.0 3.021461440612672E-4 0.0 13 0.0 0.0 0.0 3.021461440612672E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 25 0.0060208836 19.005291 5 TAGGACC 325 0.0 12.86512 4 TTAGCAC 70 1.0873037E-4 12.217688 3 TCCAACG 65 8.0443063E-4 11.687907 18 GGTATCA 885 0.0 11.641034 1 AATGTCC 275 0.0 11.049828 8 CTTAGAC 115 8.7709304E-7 10.742121 3 TTAGACC 80 3.7584023E-4 10.690476 4 AAATGTC 295 0.0 10.622583 7 GGCGAGG 385 0.0 10.361823 19 CTAGGAC 120 1.5157111E-6 10.294533 3 ATGTCCA 290 0.0 10.150839 9 ATTGGAC 75 0.002640024 10.136155 3 TAGGCCT 75 0.002640024 10.136155 4 TAGGACT 150 2.4278052E-8 10.136155 4 TAGTACC 75 0.002640024 10.136155 4 TACTGTC 85 6.614378E-4 10.054531 7 TGTAGGA 935 0.0 9.959992 2 GAAATGT 350 0.0 9.7716875 6 TTAGGAC 670 0.0 9.644477 3 >>END_MODULE