FastQCFastQC Report
Thu 26 May 2016
SRR1511967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826641
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21660.2620242644630499No Hit
GTCCTACAGTGGACATTTCTAAATT18840.2279103020537331No Hit
CTGTAGGACGTGGAATATGGCAAGA18280.22113589817103194No Hit
GTCCTAAAGTGTGTATTTCTCATTT16810.20335308797894128No Hit
CTTTAGGACGTGAAATATGGCGAGG15820.19137690968630883No Hit
GTCCTACAGTGTGCATTTCTCATTT13690.16560998063246318No Hit
TATCAACGCAGAGTACTTTTTTTTT12840.155327403310506No Hit
GGTATCAACGCAGAGTACTTTTTTT12770.15448060282516837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT350.002216584716.23293318
CGGTAGG455.4628786E-415.1978051
ACGAATG400.00539118614.20381719
GGTATCA3800.013.1123921
CGTTAGG450.00955560213.02669051
TGTCGAG802.0702591E-613.02016518
TAGGACC4400.012.48214154
GAATTAC550.002485202212.4345691
TCGAACT550.003141807912.051723519
TATGTCG802.9592333E-511.83651416
GGCGAGG4650.011.81105819
TATACTG1157.37964E-811.5277365
TAGAACC1002.0024654E-611.3630544
GTATAGA959.949126E-611.3126531
TAGCACC600.006015295611.0474134
CTCGAAC600.006015295611.04741318
GACCTAC802.9062232E-410.991271
GACGTGG5700.010.964357
AGGACGT12200.010.9439255
AGGACCT6700.010.8825275
Produced by FastQC (version 0.11.5)