##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511967_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 826641 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.524423540569607 31.0 31.0 34.0 26.0 34.0 2 27.67195554055509 31.0 27.0 34.0 10.0 34.0 3 29.618907119293624 31.0 30.0 34.0 16.0 34.0 4 34.09608766078624 37.0 35.0 37.0 30.0 37.0 5 34.550335635420936 37.0 35.0 37.0 32.0 37.0 6 34.84695653856995 37.0 35.0 37.0 32.0 37.0 7 34.942369178397875 37.0 35.0 37.0 33.0 37.0 8 34.99069245295116 37.0 35.0 37.0 33.0 37.0 9 36.73531557229801 39.0 37.0 39.0 34.0 39.0 10 36.6639968257079 39.0 37.0 39.0 33.0 39.0 11 36.68165261582718 39.0 37.0 39.0 33.0 39.0 12 36.617114321694665 39.0 37.0 39.0 33.0 39.0 13 36.618832116964924 39.0 37.0 39.0 33.0 39.0 14 37.977913023912436 40.0 38.0 41.0 34.0 41.0 15 37.94284943524456 40.0 38.0 41.0 33.0 41.0 16 37.908324169742365 40.0 38.0 41.0 33.0 41.0 17 37.89198213009033 40.0 38.0 41.0 33.0 41.0 18 37.857811311077 40.0 38.0 41.0 33.0 41.0 19 37.89139420861051 40.0 39.0 41.0 34.0 41.0 20 37.82391630707889 40.0 38.0 41.0 33.0 41.0 21 37.77357643765552 40.0 38.0 41.0 33.0 41.0 22 37.69545909288313 40.0 38.0 41.0 33.0 41.0 23 37.63111919200717 40.0 38.0 41.0 33.0 41.0 24 37.5877968791773 40.0 38.0 41.0 33.0 41.0 25 37.51458976750488 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9533486581377311 1101 2 3.8484738207687776 1101 3 1.843986479240069 1101 4 0.8697110891029212 1101 5 0.6278092810198572 1101 6 0.2475040744434196 1101 7 0.1275732103424616 1101 8 0.035732129155384484 1101 9 -0.095659270436947 1101 10 0.09445648149629449 1101 11 0.09163243640660568 1101 12 -0.06784183839540958 1101 13 0.08051154630880575 1101 14 -0.04833568575851643 1101 15 0.06401221977238691 1101 16 0.1435899761354662 1101 17 0.12270800346243504 1101 18 0.20369118146359 1101 19 0.2344226219587071 1101 20 0.023243439492254936 1101 21 0.2699437226184145 1101 22 0.15827776354922918 1101 23 0.0676617042884402 1101 24 0.1462046087519866 1101 25 0.01027924764378696 1102 1 1.6310793123453244 1102 2 4.525105614810986 1102 3 2.701805148688031 1102 4 1.7044696591479394 1102 5 1.2655898495536064 1102 6 1.0329256855373856 1102 7 0.8863561096304622 1102 8 0.8752426707630718 1102 9 0.9485372579099192 1102 10 0.7624942844145295 1102 11 0.9813925496450793 1102 12 0.8341148244753569 1102 13 0.8453794170211211 1102 14 1.155858075482847 1102 15 1.1390699564929392 1102 16 1.014416702117522 1102 17 0.9818516907369457 1102 18 1.111488033348948 1102 19 0.9945331997516149 1102 20 0.9697611436461102 1102 21 1.0333535415858606 1102 22 0.9558985849538573 1102 23 0.8729559221022143 1102 24 0.9192647081549268 1102 25 0.7655394087744725 1103 1 1.4219013121742563 1103 2 4.405101444936722 1103 3 2.5612803860501074 1103 4 1.5488798892394584 1103 5 1.083406439237983 1103 6 0.8925853596456719 1103 7 0.6023555964151726 1103 8 0.5728065019152382 1103 9 0.60752173314728 1103 10 0.47413411419812235 1103 11 0.5199478485929845 1103 12 0.49140462004459806 1103 13 0.6496679723200671 1103 14 0.6341453263595866 1103 15 0.7584566642168298 1103 16 0.5987657740531986 1103 17 0.5556210432303104 1103 18 0.5767283891782213 1103 19 0.6158016968861659 1103 20 0.6660312445784484 1103 21 0.7019180356411141 1103 22 0.6761935768279486 1103 23 0.8301265020354975 1103 24 0.8023852709810342 1103 25 0.8124905891696486 1104 1 1.6510679788409242 1104 2 4.400471815307096 1104 3 2.4733174230871455 1104 4 1.7537410003505727 1104 5 1.307943476275021 1104 6 0.979390915201229 1104 7 0.8153185593781345 1104 8 0.7475750204337572 1104 9 0.9790495109250585 1104 10 0.8838563364203438 1104 11 0.9018922930374274 1104 12 0.9555249904149719 1104 13 0.9772142686163647 1104 14 1.1156268078410747 1104 15 1.0628548123649821 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-0.7519305601746922 2315 14 -0.9768129196222404 2315 15 -1.2064280317999376 2315 16 -0.8212377200068985 2315 17 -0.9481438065251027 2315 18 -0.8333519742040281 2315 19 -0.6959732856435323 2315 20 -0.7535136540938083 2315 21 -0.6946839909137381 2315 22 -0.9190850744648529 2315 23 -0.8108981310881944 2315 24 -0.9900762943578911 2315 25 -0.7085654904949266 2316 1 -0.6451855636197443 2316 2 -0.2373146353916269 2316 3 -0.07038921230814665 2316 4 -0.3749449910498015 2316 5 -0.7501742555729223 2316 6 -0.8136683049688074 2316 7 -0.9940237644760614 2316 8 -1.095023679849639 2316 9 -1.206370820356092 2316 10 -1.3479702295915814 2316 11 -1.3420427699939381 2316 12 -1.339351267769338 2316 13 -1.45028438769166 2316 14 -1.3600126673861368 2316 15 -1.253929732734207 2316 16 -1.300371820005239 2316 17 -1.5553740217555188 2316 18 -1.5478074263206594 2316 19 -1.4383124436395818 2316 20 -1.4178670501542783 2316 21 -1.5173786026858664 2316 22 -1.6693215458144834 2316 23 -1.6616739230597872 2316 24 -1.766918696574912 2316 25 -1.7957346380364498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17874.0 3 1.0 4 152.0 5 708.0 6 805.0 7 753.0 8 678.0 9 639.0 10 601.0 11 615.0 12 558.0 13 599.0 14 622.0 15 610.0 16 608.0 17 674.0 18 800.0 19 909.0 20 1177.0 21 1524.0 22 1793.0 23 2073.0 24 2385.0 25 2761.0 26 3170.0 27 3697.0 28 4362.0 29 5428.0 30 7094.0 31 9389.0 32 13258.0 33 19444.0 34 30542.0 35 50288.0 36 93129.0 37 141414.0 38 405507.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.19592543800755 19.658231324117725 14.064025374981401 26.081817862893324 2 15.425602288584289 21.767137547210787 41.510171069678506 21.29708909452642 3 17.51897135515901 25.19872592818406 30.97245358021197 26.309849136444964 4 11.348699132997274 17.730187590501803 40.36298707661488 30.558126199886043 5 11.98960612889997 38.25530066860947 37.27107656165131 12.484016640839252 6 29.13175126808373 39.05492226976402 19.336205196693605 12.477121265458644 7 25.59575438430951 32.25499340100479 24.15982270417267 17.989429510513027 8 22.650851640375496 38.80988096390206 22.061598761250362 16.477668634472078 9 25.006744161008225 15.777949557304805 21.8550737260794 37.360232555607574 10 13.776993342922363 29.411145614954048 36.22718018385292 20.58468085827066 11 31.74872768227078 22.810264673540267 27.19971547503693 18.241292169152025 12 21.979432425926127 24.880207974199198 34.0434360260379 19.09692357383677 13 27.51557205606787 22.925550511044094 27.21386914029186 22.34500829259618 14 19.01309032578834 23.442703664589587 29.304619538590515 28.239586471031558 15 20.90714106849285 32.73065333076874 24.714839936562548 21.64736566417586 16 19.75234714948811 28.910736341410598 30.647887051331836 20.689029457769458 17 19.689441970577313 29.545957676911744 29.995971649119753 20.768628703391194 18 19.36634286198178 28.144998366863046 32.71995935303582 19.76869941811935 19 21.867340890120126 27.209755271403168 29.348075922722376 21.574827915754327 20 21.677697357835942 28.248085909950365 30.302927659553873 19.771289072659815 21 20.799268333401887 27.62464826047718 28.902645274774198 22.673438131346735 22 20.972336979072786 29.44689684706645 29.41495983073381 20.16580634312695 23 20.055163737101243 28.883902108561266 29.948949348559818 21.111984805777677 24 21.266089228684795 27.449433852704924 30.843414303687215 20.441062614923062 25 20.82691677241643 28.2275049386853 30.268787871822823 20.676790417075452 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 262.0 2 290.0 3 318.0 4 318.0 5 318.0 6 739.5 7 1161.0 8 1161.0 9 1161.0 10 1672.5 11 2184.0 12 2184.0 13 2184.0 14 3754.5 15 5325.0 16 5325.0 17 5325.0 18 9267.0 19 13209.0 20 13209.0 21 13209.0 22 20825.0 23 28441.0 24 28441.0 25 28441.0 26 40375.0 27 52309.0 28 52309.0 29 52309.0 30 67074.5 31 81840.0 32 81840.0 33 81840.0 34 89428.5 35 97017.0 36 97017.0 37 97017.0 38 100755.0 39 104493.0 40 104493.0 41 104493.0 42 105932.0 43 107371.0 44 107371.0 45 107371.0 46 104941.0 47 102511.0 48 102511.0 49 102511.0 50 94742.5 51 86974.0 52 86974.0 53 86974.0 54 74030.0 55 61086.0 56 61086.0 57 61086.0 58 50450.5 59 39815.0 60 39815.0 61 39815.0 62 31348.0 63 22881.0 64 22881.0 65 22881.0 66 17224.0 67 11567.0 68 11567.0 69 11567.0 70 8283.5 71 5000.0 72 5000.0 73 5000.0 74 3436.5 75 1873.0 76 1873.0 77 1873.0 78 1278.5 79 684.0 80 684.0 81 684.0 82 449.5 83 215.0 84 215.0 85 215.0 86 146.0 87 77.0 88 77.0 89 77.0 90 47.5 91 18.0 92 18.0 93 18.0 94 11.5 95 5.0 96 5.0 97 5.0 98 5.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.078361707198167 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.2097149790537851E-4 9 0.0 10 2.4194299581075702E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0013306864769591635 19 0.0013306864769591635 20 9.677719832430281E-4 21 0.004234002426688248 22 0.0016936009706752992 23 0.0013306864769591635 24 1.2097149790537851E-4 25 4.8388599162151405E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 826641.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.912846636949 #Duplication Level Percentage of deduplicated Percentage of total 1 88.62290840782845 69.04863071299515 2 8.21599166258307 12.802625967545728 3 1.7177683142967834 4.01508657688847 4 0.5680105582293861 1.7702127804597578 5 0.2718928975394876 1.059197481383489 6 0.14568337714874127 0.6810363972805608 7 0.08647843561013376 0.47164467637669344 8 0.06131493904432419 0.3821777153851445 9 0.04324667666951238 0.30325245182184946 >10 0.22685309153833208 3.248680768429281 >50 0.019848436486339098 1.0681245584765042 >100 0.016853092377816313 2.64001179942354 >500 0.0018900663886452927 0.9052836328021819 >1k 0.001260044259096862 1.6040344807316416 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2166 0.2620242644630499 No Hit GTCCTACAGTGGACATTTCTAAATT 1884 0.2279103020537331 No Hit CTGTAGGACGTGGAATATGGCAAGA 1828 0.22113589817103194 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1681 0.20335308797894128 No Hit CTTTAGGACGTGAAATATGGCGAGG 1582 0.19137690968630883 No Hit GTCCTACAGTGTGCATTTCTCATTT 1369 0.16560998063246318 No Hit TATCAACGCAGAGTACTTTTTTTTT 1284 0.155327403310506 No Hit GGTATCAACGCAGAGTACTTTTTTT 1277 0.15448060282516837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.4194299581075702E-4 0.0 5 0.0 0.0 0.0 2.4194299581075702E-4 0.0 6 0.0 0.0 0.0 2.4194299581075702E-4 0.0 7 0.0 0.0 0.0 2.4194299581075702E-4 0.0 8 0.0 0.0 0.0 2.4194299581075702E-4 0.0 9 0.0 0.0 0.0 3.6291449371613556E-4 0.0 10 0.0 0.0 0.0 4.8388599162151405E-4 0.0 11 0.0 0.0 0.0 4.8388599162151405E-4 0.0 12 0.0 0.0 0.0 4.8388599162151405E-4 0.0 13 0.0 0.0 0.0 4.8388599162151405E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAAT 35 0.0022165847 16.232933 18 CGGTAGG 45 5.4628786E-4 15.197805 1 ACGAATG 40 0.005391186 14.203817 19 GGTATCA 380 0.0 13.112392 1 CGTTAGG 45 0.009555602 13.0266905 1 TGTCGAG 80 2.0702591E-6 13.020165 18 TAGGACC 440 0.0 12.4821415 4 GAATTAC 55 0.0024852022 12.434569 1 TCGAACT 55 0.0031418079 12.0517235 19 TATGTCG 80 2.9592333E-5 11.836514 16 GGCGAGG 465 0.0 11.811058 19 TATACTG 115 7.37964E-8 11.527736 5 TAGAACC 100 2.0024654E-6 11.363054 4 GTATAGA 95 9.949126E-6 11.312653 1 TAGCACC 60 0.0060152956 11.047413 4 CTCGAAC 60 0.0060152956 11.047413 18 GACCTAC 80 2.9062232E-4 10.99127 1 GACGTGG 570 0.0 10.96435 7 AGGACGT 1220 0.0 10.943925 5 AGGACCT 670 0.0 10.882527 5 >>END_MODULE