FastQCFastQC Report
Thu 26 May 2016
SRR1511967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826641
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32440.3924315392050479No Hit
TATCAACGCAGAGTACTTTTTTTTT23560.2850088490650718No Hit
GGTATCAACGCAGAGTACTTTTTTT21970.2657743808981166No Hit
CTGTAGGACGTGGAATATGGCAAGA17120.207103204414008No Hit
CTTTAGGACGTGAAATATGGCGAGG15030.18182016135178392No Hit
GTCCTAAAGTGTGTATTTCTCATTT14570.1762554724481365No Hit
GTCCTACAGTGGACATTTCTAAATT14520.1756506149586096No Hit
GTCCTACAGTGTGCATTTCTCATTT11340.13718167862469924No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10200.12339092786348609No Hit
CTGTAGGACCTGGAATATGGCGAGA9130.11044697758761059No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8330.1007692577551803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACAC704.4253102E-714.929573
TAGGACC4500.013.5120154
AGGACCT6950.012.1662825
GGTATCA7300.012.1461771
CTGATCA550.00307316512.0873299
GGACCTG6900.011.9768886
ACTGTTC2950.011.9117268
CGTGATT1201.0017175E-811.87220318
GACGTGG5800.011.7896127
ATTGCAC658.0119516E-411.69308953
TGTAGGA13750.011.4698582
GGGTAAG752.0037562E-411.4409161
CTGTAGG14150.011.25225451
GATATAC3300.010.9786571
AATGCCG1053.4689856E-610.85458618
ATACACT3600.010.8201684
GTAGGAC13550.010.797773
AATCCCG1502.1318556E-910.7641319
TTAGAGG803.7643203E-410.6882153
ACGTGGA6500.010.6660718
Produced by FastQC (version 0.11.5)