##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511967_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 826641 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.710634967295356 31.0 28.0 31.0 23.0 34.0 2 28.909400816073724 31.0 28.0 33.0 23.0 34.0 3 28.906463628104582 31.0 28.0 34.0 23.0 34.0 4 32.39116859676692 35.0 32.0 37.0 26.0 37.0 5 31.90228527256693 35.0 32.0 37.0 22.0 37.0 6 31.91357433275146 35.0 32.0 37.0 22.0 37.0 7 31.671438992259034 35.0 32.0 37.0 19.0 37.0 8 31.515270837038084 35.0 32.0 37.0 18.0 37.0 9 32.64396999422966 37.0 32.0 39.0 17.0 39.0 10 32.26682441349993 37.0 32.0 39.0 17.0 39.0 11 32.36306207894358 37.0 32.0 39.0 17.0 39.0 12 32.05860585187524 37.0 32.0 39.0 13.0 39.0 13 32.074401100356745 37.0 32.0 39.0 11.0 39.0 14 32.57714049992681 37.0 32.0 40.0 10.0 41.0 15 32.497092450047845 37.0 31.0 40.0 10.0 41.0 16 32.31196371822835 37.0 31.0 40.0 10.0 41.0 17 32.202219585043565 37.0 31.0 40.0 10.0 41.0 18 32.03136911912184 37.0 31.0 40.0 8.0 41.0 19 32.07521281910769 37.0 31.0 40.0 2.0 41.0 20 31.884827875704204 37.0 31.0 40.0 2.0 41.0 21 31.694005015478304 37.0 30.0 40.0 2.0 41.0 22 31.484192049511215 37.0 30.0 40.0 2.0 41.0 23 31.493804444734778 37.0 31.0 40.0 2.0 41.0 24 31.242776489431325 37.0 30.0 40.0 2.0 41.0 25 30.62554361566871 36.0 30.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28371176801893583 1101 2 0.1848854982938164 1101 3 0.3649187669149576 1101 4 0.1064780394168281 1101 5 0.13787470992732764 1101 6 0.30175417067635024 1101 7 0.2827715465469822 1101 8 0.11126173785548588 1101 9 0.22764723849638102 1101 10 0.18978812627432973 1101 11 -0.040820671410926934 1101 12 0.4187287955676311 1101 13 -0.07174077602822848 1101 14 -0.14608575606009566 1101 15 -0.0817986989007835 1101 16 0.21497179525276522 1101 17 0.27023152427491937 1101 18 0.2380879744167359 1101 19 0.41536630864530366 1101 20 0.5921894692198428 1101 21 0.7033006409740175 1101 22 0.45615532059921193 1101 23 0.1012172141620411 1101 24 -0.0027330837030135058 1101 25 -0.1723904766277613 1102 1 0.38880590281954497 1102 2 0.23128240764982522 1102 3 0.12416462423874108 1102 4 0.24851037198033765 1102 5 0.15296607762243397 1102 6 0.4939083623090461 1102 7 0.5355519554400345 1102 8 0.25478405030427 1102 9 0.6419521096663345 1102 10 0.7693716768963981 1102 11 0.4795468214850658 1102 12 0.7708349885625978 1102 13 0.700765902274469 1102 14 0.7128264521965164 1102 15 0.4445127494660923 1102 16 0.8431819037020176 1102 17 0.4595840215876308 1102 18 0.5724341393149359 1102 19 0.36571538964699357 1102 20 0.6083318048697386 1102 21 0.3392825909319761 1102 22 0.423639170281529 1102 23 0.17989347346111018 1102 24 0.24484303830505283 1102 25 0.603436728276403 1103 1 -0.21973378582984537 1103 2 0.059144117512111904 1103 3 0.028129212383468882 1103 4 0.18865396534275192 1103 5 0.17391434979761655 1103 6 0.1545447492733807 1103 7 0.15177116252240452 1103 8 0.11077637345883318 1103 9 0.09868431820281387 1103 10 0.08543478099356605 1103 11 0.373851200922239 1103 12 0.45096019171031543 1103 13 0.4837934448285708 1103 14 0.19029843919506817 1103 15 0.2012700841679944 1103 16 0.10508742932001525 1103 17 0.2505075952767939 1103 18 -0.12523661169010936 1103 19 0.12601979046814193 1103 20 0.14190998466616733 1103 21 0.18191260547307664 1103 22 0.49166692667533596 1103 23 0.17560064136804598 1103 24 0.1914820127298249 1103 25 0.5992909109810469 1104 1 0.48049769565163913 1104 2 0.3635422656602607 1104 3 0.4829903234945796 1104 4 0.6435150764538662 1104 5 0.7537754609087237 1104 6 0.7401928974215259 1104 7 0.6899656069668509 1104 8 0.5887856327180891 1104 9 0.785026910795402 1104 10 0.7451570032157875 1104 11 0.8669067564777961 1104 12 0.791237969488094 1104 13 0.9895804818656089 1104 14 0.8268725132691372 1104 15 0.9304367508346658 1104 16 1.3365689108014962 1104 17 1.381294632313832 1104 18 1.350457832754337 1104 19 1.3528716423199896 1104 20 1.2229285031846864 1104 21 1.3219589017693743 1104 22 1.2127317414901526 1104 23 1.1929617524791603 1104 24 0.9461116423594547 1104 25 0.7080872072773445 1105 1 0.5815023730094069 1105 2 0.5005220289286818 1105 3 0.3417030955449789 1105 4 0.30407254113504223 1105 5 0.5489024177624877 1105 6 0.5629067194910213 1105 7 0.3540549540458855 1105 8 0.578357034016296 1105 9 0.4355853300393946 1105 10 0.429996159382263 1105 11 0.5541442517049759 1105 12 0.47679304776224285 1105 13 0.641916774343656 1105 14 0.8492808752393586 1105 15 0.8518046393417293 1105 16 0.8248516523058882 1105 17 0.39113856129436186 1105 18 0.2960954807115712 1105 19 0.5258980837098335 1105 20 0.8688811568692962 1105 21 0.7366329185696792 1105 22 1.0178935398864866 1105 23 0.8012926994054688 1105 24 1.147321550721422 1105 25 1.0358960083464552 1106 1 -0.4299402378649191 1106 2 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13 0.16931503908719492 1107 14 0.04354398577971352 1107 15 0.40654369688105874 1107 16 0.28015631308267075 1107 17 0.38844332512429247 1107 18 0.2730036484662435 1107 19 0.5382050076922837 1107 20 0.567379355421 1107 21 0.32888332186420755 1107 22 0.4479898153079134 1107 23 0.3556195443540773 1107 24 0.3781209656966311 1107 25 0.5882014261675153 1108 1 -0.16700804444535322 1108 2 -0.20393337916184962 1108 3 -0.145779632730477 1108 4 -0.26721836846266456 1108 5 -0.45520430390823563 1108 6 -0.23820663478739235 1108 7 -0.19605859800289593 1108 8 -0.22451368919942283 1108 9 0.03004590139666874 1108 10 -0.2965405097664906 1108 11 -0.34256386710059417 1108 12 0.03785962735685189 1108 13 -0.07060872675204521 1108 14 -0.21324110846383348 1108 15 0.21595829497439922 1108 16 -0.20151153638961006 1108 17 0.08071606272454801 1108 18 -0.12070756787224468 1108 19 -0.14951693542345268 1108 20 0.30186288368337344 1108 21 0.16900275181872715 1108 22 -0.041408370522209026 1108 23 0.08248500255426094 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-1.0558330517674754 2315 4 -0.9079873693882021 2315 5 -0.920586873123419 2315 6 -0.6884321899299763 2315 7 -0.5667429884209945 2315 8 -0.6634593008947682 2315 9 -0.40724248123813567 2315 10 -0.7164301735836531 2315 11 -0.7284985544935552 2315 12 -0.3124351611897538 2315 13 -0.6105287075124473 2315 14 -0.7538140695400841 2315 15 -0.8193099298082451 2315 16 -0.8207598802537106 2315 17 -0.6168840888601714 2315 18 -0.7520404202156143 2315 19 -0.47069142546477494 2315 20 -0.7225990998900897 2315 21 -0.6555000430244782 2315 22 -0.7461125981324912 2315 23 -0.4718923705117568 2315 24 -0.5994203282904422 2315 25 -0.6307274328344654 2316 1 -0.7339219515681421 2316 2 -1.03954089665773 2316 3 -0.8924952379243614 2316 4 -1.0386406940924076 2316 5 -0.7067138872785144 2316 6 -1.1543089593164773 2316 7 -1.0708816519015194 2316 8 -1.3056673624934803 2316 9 -0.9249371759826666 2316 10 -1.2764855991491117 2316 11 -1.2768316389096803 2316 12 -1.431115568086419 2316 13 -1.22016678188891 2316 14 -1.5220904008322975 2316 15 -0.9641017520481654 2316 16 -0.9728283822699417 2316 17 -1.1639908411543587 2316 18 -0.9322183130484589 2316 19 -1.3469994854040372 2316 20 -1.2641964845999354 2316 21 -1.3760921340949892 2316 22 -1.5580191380173254 2316 23 -1.3508273367419896 2316 24 -1.3469933854916185 2316 25 -1.485517429681515 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36555.0 3 0.0 4 569.0 5 4131.0 6 5111.0 7 4288.0 8 3470.0 9 3081.0 10 2966.0 11 2891.0 12 2730.0 13 2585.0 14 2553.0 15 2442.0 16 2591.0 17 2828.0 18 2982.0 19 3368.0 20 3940.0 21 5160.0 22 6483.0 23 7889.0 24 9285.0 25 10944.0 26 12317.0 27 14324.0 28 16746.0 29 19849.0 30 25253.0 31 31634.0 32 39668.0 33 50684.0 34 64621.0 35 83612.0 36 107272.0 37 130813.0 38 101006.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.64050838914967 23.948213776058587 16.200197963285703 20.211079871506037 2 16.480552017139242 25.101948729859757 39.99414497950138 18.42335427349962 3 20.64342320305913 28.290878386143444 31.281656728858113 19.784041681939318 4 15.423140153948328 21.44848852161942 38.350263294465194 24.778108029967058 5 12.729180305650381 40.32617390052144 36.1843195957254 10.760326198102785 6 29.59302227073919 41.16226100890007 18.159087773482792 11.085628946877959 7 26.494814556742284 33.753709288554525 23.64629869556434 16.105177459138854 8 24.202283699937457 40.01446819114948 21.103961695584903 14.679286413328155 9 26.761435738125737 16.082918703524264 21.56788739005203 35.58775816829797 10 14.87078429451237 31.689693591292954 35.03382967938924 18.405692434805434 11 33.71524035222062 23.504399128521328 26.186821123075195 16.593539396182866 12 22.949865782123073 26.59231758405402 33.156110088901954 17.30170654492095 13 29.33449949857314 23.688033862341694 26.64119006920779 20.33627656987737 14 20.472611448016735 24.74459892504727 28.893316445712223 25.88947318122377 15 22.403316554586574 33.9759339302067 24.071513510701745 19.549236004504976 16 20.989885573060132 30.356103798384066 29.51704548891236 19.13696513964345 17 20.918633360793866 30.92793606898279 28.90130056457398 19.252130005649366 18 20.565517558408065 29.16719591697 31.957282544659655 18.310003979962282 19 23.344474808290418 28.216118000437916 28.989004900555383 19.450402290716283 20 23.10171295848253 29.197837027000872 29.645432110713248 18.05501790380335 21 22.456423042167035 28.31567754321404 28.59996056329168 20.627938851327237 22 22.58295922897606 30.009278513889342 29.031707839315978 18.37605441781862 23 21.642163889765932 29.814756345257493 29.363169743576716 19.179910021399856 24 22.547283254336364 28.79772179033987 30.044857696579058 18.61013725874471 25 22.244805371563377 29.28881226748934 29.6933732571152 18.773009103832077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 307.0 1 307.0 2 364.5 3 422.0 4 422.0 5 422.0 6 906.5 7 1391.0 8 1391.0 9 1391.0 10 2163.0 11 2935.0 12 2935.0 13 2935.0 14 4644.5 15 6354.0 16 6354.0 17 6354.0 18 11143.5 19 15933.0 20 15933.0 21 15933.0 22 24329.0 23 32725.0 24 32725.0 25 32725.0 26 45680.5 27 58636.0 28 58636.0 29 58636.0 30 73276.0 31 87916.0 32 87916.0 33 87916.0 34 95116.5 35 102317.0 36 102317.0 37 102317.0 38 103967.0 39 105617.0 40 105617.0 41 105617.0 42 105466.5 43 105316.0 44 105316.0 45 105316.0 46 101071.5 47 96827.0 48 96827.0 49 96827.0 50 89129.0 51 81431.0 52 81431.0 53 81431.0 54 68367.0 55 55303.0 56 55303.0 57 55303.0 58 45435.0 59 35567.0 60 35567.0 61 35567.0 62 27818.0 63 20069.0 64 20069.0 65 20069.0 66 15228.0 67 10387.0 68 10387.0 69 10387.0 70 7459.5 71 4532.0 72 4532.0 73 4532.0 74 3155.0 75 1778.0 76 1778.0 77 1778.0 78 1204.0 79 630.0 80 630.0 81 630.0 82 404.0 83 178.0 84 178.0 85 178.0 86 117.0 87 56.0 88 56.0 89 56.0 90 32.5 91 9.0 92 9.0 93 9.0 94 6.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3938831971799124 2 0.0 3 0.0 4 0.0 5 0.032904247430262955 6 0.04294488175640937 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.2097149790537851E-4 21 0.0 22 0.0 23 0.0 24 0.0032662304434452195 25 0.007984118861754982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 826641.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.24888317506368 #Duplication Level Percentage of deduplicated Percentage of total 1 89.8894870198325 70.33751968481278 2 7.197191111347359 11.263443329208526 3 1.5652552764433652 3.6743843179670668 4 0.5156052031858096 1.6138212523416555 5 0.23696629184947848 0.9271173843678944 6 0.14838880731429174 0.6966755068813829 7 0.08571096759904429 0.46947512433355 8 0.055261215776940896 0.34593027339534677 9 0.03942881135240476 0.277673440792135 >10 0.22358534212520526 3.198678113526975 >50 0.021484144583103076 1.1728756916529155 >100 0.01854497884437975 3.091256621975134 >500 0.0016999563603825394 0.9872479288911168 >1k 0.0013908733857675322 1.9439013298535888 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3244 0.3924315392050479 No Hit TATCAACGCAGAGTACTTTTTTTTT 2356 0.2850088490650718 No Hit GGTATCAACGCAGAGTACTTTTTTT 2197 0.2657743808981166 No Hit CTGTAGGACGTGGAATATGGCAAGA 1712 0.207103204414008 No Hit CTTTAGGACGTGAAATATGGCGAGG 1503 0.18182016135178392 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1457 0.1762554724481365 No Hit GTCCTACAGTGGACATTTCTAAATT 1452 0.1756506149586096 No Hit GTCCTACAGTGTGCATTTCTCATTT 1134 0.13718167862469924 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1020 0.12339092786348609 No Hit CTGTAGGACCTGGAATATGGCGAGA 913 0.11044697758761059 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 833 0.1007692577551803 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.2097149790537851E-4 0.0 12 0.0 0.0 0.0 1.2097149790537851E-4 0.0 13 0.0 0.0 0.0 1.2097149790537851E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACAC 70 4.4253102E-7 14.92957 3 TAGGACC 450 0.0 13.512015 4 AGGACCT 695 0.0 12.166282 5 GGTATCA 730 0.0 12.146177 1 CTGATCA 55 0.003073165 12.087329 9 GGACCTG 690 0.0 11.976888 6 ACTGTTC 295 0.0 11.911726 8 CGTGATT 120 1.0017175E-8 11.872203 18 GACGTGG 580 0.0 11.789612 7 ATTGCAC 65 8.0119516E-4 11.6930895 3 TGTAGGA 1375 0.0 11.469858 2 GGGTAAG 75 2.0037562E-4 11.440916 1 CTGTAGG 1415 0.0 11.2522545 1 GATATAC 330 0.0 10.978657 1 AATGCCG 105 3.4689856E-6 10.854586 18 ATACACT 360 0.0 10.820168 4 GTAGGAC 1355 0.0 10.79777 3 AATCCCG 150 2.1318556E-9 10.76413 19 TTAGAGG 80 3.7643203E-4 10.688215 3 ACGTGGA 650 0.0 10.666071 8 >>END_MODULE