FastQCFastQC Report
Thu 26 May 2016
SRR1511966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149874
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22450.19523878268401582No Hit
GTCCTACAGTGGACATTTCTAAATT21530.18723790606622986No Hit
CTGTAGGACGTGGAATATGGCAAGA19380.16854020527466487No Hit
GTCCTAAAGTGTGTATTTCTCATTT18450.1604523626066856No Hit
CTTTAGGACGTGAAATATGGCGAGG16670.14497240567227365No Hit
GGTATCAACGCAGAGTACTTTTTTT13950.12131764001968912No Hit
TATCAACGCAGAGTACTTTTTTTTT13240.11514305045596299No Hit
GTCCTACAGTGTGCATTTCTCATTT11770.10235904107754415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG358.1073355E-519.5101431
CCGGTTT1059.276846E-1114.43179612
CCAACGA1250.014.39571619
GTTGCGG400.00538663614.20629917
TCCAACG1650.013.77580518
CGTACAC500.001534231113.2592123
GCCGGTT1153.947207E-1013.17685711
CGGTTTC1301.4551915E-1113.11350613
CTAGGGT1052.0785592E-812.6278224
GTAGAAC3750.012.1227083
TGCCGGT1251.4788384E-912.12270810
GTACTAA854.0602234E-511.4765561
TAGGACC3800.011.4647324
TAGAACA4350.011.3214944
GGGTAAT700.001194929911.1486541
CTTACAC1303.4120603E-810.9279223
GGTATCA6400.010.8220331
CACCTTT3600.010.78626414
GTAGGAC11200.010.73928453
GAACAGT2750.010.6762496
Produced by FastQC (version 0.11.5)