##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511966_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1149874 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.469030519865655 31.0 30.0 34.0 25.0 34.0 2 27.673399868159468 31.0 27.0 34.0 10.0 34.0 3 29.583493495809105 31.0 30.0 34.0 16.0 34.0 4 34.05142563446082 37.0 35.0 37.0 30.0 37.0 5 34.494032389635734 37.0 35.0 37.0 32.0 37.0 6 34.787349744406775 37.0 35.0 37.0 32.0 37.0 7 34.885771832392074 37.0 35.0 37.0 32.0 37.0 8 34.933815357160874 37.0 35.0 37.0 33.0 37.0 9 36.669377688338024 39.0 37.0 39.0 33.0 39.0 10 36.597985518413324 39.0 37.0 39.0 33.0 39.0 11 36.61858168808061 39.0 37.0 39.0 33.0 39.0 12 36.5472356101625 39.0 37.0 39.0 33.0 39.0 13 36.547226043896984 39.0 37.0 39.0 33.0 39.0 14 37.89466237170333 40.0 38.0 41.0 33.0 41.0 15 37.85331436313892 40.0 38.0 41.0 33.0 41.0 16 37.81658773048177 40.0 38.0 41.0 33.0 41.0 17 37.79544541401928 40.0 38.0 41.0 33.0 41.0 18 37.75829264771618 40.0 38.0 41.0 33.0 41.0 19 37.79659597486333 40.0 38.0 41.0 33.0 41.0 20 37.72130685622947 40.0 38.0 41.0 33.0 41.0 21 37.67591144768905 40.0 38.0 41.0 33.0 41.0 22 37.59299192781122 40.0 38.0 41.0 33.0 41.0 23 37.52566368141205 40.0 38.0 41.0 33.0 41.0 24 37.46844175970585 40.0 38.0 41.0 33.0 41.0 25 37.38918090155965 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2473639914997463 1101 2 4.307199235592105 1101 3 2.259871889151732 1101 4 1.3400290082450965 1101 5 0.8992015977487497 1101 6 0.7287284945083172 1101 7 0.4257878372676842 1101 8 0.2158049509928759 1101 9 0.1850807440556892 1101 10 0.39451079094367003 1101 11 0.47193355500390055 1101 12 0.41384101325309075 1101 13 0.4655692913997598 1101 14 0.39127996198173065 1101 15 0.5244549734208945 1101 16 0.5388749043939143 1101 17 0.5569852957513248 1101 18 0.5342876353648904 1101 19 0.49579654703703824 1101 20 0.5524489821934182 1101 21 0.49101553650628915 1101 22 0.5866578232579016 1101 23 0.3206212661036787 1101 24 0.480985495178885 1101 25 0.3543418016574904 1102 1 1.2175509445625927 1102 2 4.146707986586538 1102 3 2.2345847471469575 1102 4 1.371704749096331 1102 5 0.8899235547893269 1102 6 0.6691030221535073 1102 7 0.5463738058436576 1102 8 0.5005105993620091 1102 9 0.4625345030055712 1102 10 0.38497357435655744 1102 11 0.562759305799446 1102 12 0.5190574263398062 1102 13 0.5550227271595105 1102 14 0.6128379870413951 1102 15 0.6744381923946463 1102 16 0.5923827604002838 1102 17 0.5957730841363684 1102 18 0.6181163197324366 1102 19 0.7015580327172302 1102 20 0.6416606468712232 1102 21 0.625420369441855 1102 22 0.6004971977606886 1102 23 0.7616142056422603 1102 24 0.73147214494022 1102 25 0.6894887288651219 1103 1 1.3351693500497852 1103 2 4.263237454662239 1103 3 2.466517131468514 1103 4 1.595929939096159 1103 5 1.0347315425872097 1103 6 1.027613566415333 1103 7 0.7986367832881669 1103 8 0.6621209478407906 1103 9 0.7343099599737357 1103 10 0.5856276890918153 1103 11 0.6764714539400742 1103 12 0.6101896391396124 1103 13 0.7246186166558672 1103 14 0.7251095029588726 1103 15 0.814920522081259 1103 16 0.7126116065215768 1103 17 0.822730961629965 1103 18 0.7742951462001955 1103 19 0.6791680501891264 1103 20 0.6591927272682767 1103 21 0.6891316712580675 1103 22 0.6895925898063666 1103 23 0.7428164000674329 1103 24 0.8079967983703682 1103 25 0.8585072370396816 1104 1 1.1308789228960343 1104 2 3.923290841726086 1104 3 2.134517520709437 1104 4 1.357731666638962 1104 5 0.9757653588464095 1104 6 0.707501068156418 1104 7 0.5458228957826137 1104 8 0.4862851769815748 1104 9 0.3928617166142061 1104 10 0.524646611161586 1104 11 0.40339604995951106 1104 12 0.4629878806865051 1104 13 0.5261076539905645 1104 14 0.47425136028681436 1104 15 0.43036384650402937 1104 16 0.5945170553220862 1104 17 0.5683200415140348 1104 18 0.6420982437184009 1104 19 0.7226390107174296 1104 20 0.6187612804177931 1104 21 0.5235039344078274 1104 22 0.5721734860908327 1104 23 0.5584516736904419 1104 24 0.5900962952192401 1104 25 0.5593622314395716 1105 1 1.5531332222689755 1105 2 4.382359492002362 1105 3 2.569927978895322 1105 4 1.63720448901433 1105 5 1.260259290056446 1105 6 0.983836667585237 1105 7 0.8834400808574259 1105 8 0.7742985407364671 1105 9 0.8125647184146629 1105 10 0.8020628742770057 1105 11 0.6767612793628714 1105 12 0.8710325196633448 1105 13 0.9328970157587335 1105 14 0.9310636158278811 1105 15 0.8645791721388463 1105 16 0.9740511864452017 1105 17 1.0412801675461907 1105 18 0.9056618340828422 1105 19 1.0838173803703768 1105 20 1.0213191745011088 1105 21 1.092858485224241 1105 22 1.1545464450527732 1105 23 0.9981052404626496 1105 24 1.1316465528711888 1105 25 1.2370741734523563 1106 1 0.4002941660633361 1106 2 2.3508297426577762 1106 3 1.1697885038649467 1106 4 0.29611210778790564 1106 5 0.007192038314009608 1106 6 -0.17401789472692997 1106 7 -0.20595182399582512 1106 8 -0.31152256355409236 1106 9 -0.18997900241147647 1106 10 -0.2624373335367949 1106 11 -0.34609624029950936 1106 12 -0.16208180989736576 1106 13 -0.4237614182843572 1106 14 -0.34404188963922167 1106 15 -0.40471753800886034 1106 16 -0.491629563602757 1106 17 -0.5646948788122685 1106 18 -0.3825978852086749 1106 19 -0.5541709820568883 1106 20 -0.3542802883801528 1106 21 -0.41267056075056274 1106 22 -0.3548471382965417 1106 23 -0.4055970422920012 1106 24 -0.3745079327097329 1106 25 -0.3850583957656184 1107 1 1.2229368229236393 1107 2 4.074445553104383 1107 3 2.2061970732761154 1107 4 1.2735076805036911 1107 5 0.993330075653823 1107 6 0.6754283206130651 1107 7 0.4573626122267669 1107 8 0.4304121523022033 1107 9 0.43410386189530215 1107 10 0.32536824798131647 1107 11 0.3515942035309081 1107 12 0.3987875660354163 1107 13 0.41204663387112106 1107 14 0.34779847671168085 1107 15 0.41233217268440114 1107 16 0.41719425791274034 1107 17 0.3745282581081142 1107 18 0.3972568968215242 1107 19 0.2799439488373707 1107 20 0.4116628733554464 1107 21 0.4340852991904143 1107 22 0.44718487931355355 1107 23 0.3895400467910761 1107 24 0.3931031813490975 1107 25 0.3699335569766333 1108 1 1.160747829883583 1108 2 3.6346824005752723 1108 3 2.1587407864581287 1108 4 1.347828355888197 1108 5 0.822055133102289 1108 6 0.5790678253164003 1108 7 0.47907592649327313 1108 8 0.6034312136191389 1108 9 0.5268631514630968 1108 10 0.568792672256798 1108 11 0.6509502848018798 1108 12 0.4971980208288471 1108 13 0.5731213747330983 1108 14 0.6126551724194371 1108 15 0.7038452301549043 1108 16 0.7156741468181593 1108 17 0.6866359691519932 1108 18 0.5751046597756684 1108 19 0.6031776497306467 1108 20 0.6387757919577268 1108 21 0.5960555196712747 1108 22 0.7226716069279391 1108 23 0.6239146162720317 1108 24 0.4527421239667575 1108 25 0.6019165070446917 1109 1 1.2742379267637993 1109 2 3.4168883326960255 1109 3 2.1817921404294154 1109 4 1.3357900282621316 1109 5 0.9523745108662993 1109 6 0.8632512266207542 1109 7 0.7446203543034571 1109 8 0.6981303342978151 1109 9 0.558552847973921 1109 10 0.6325391661566186 1109 11 0.6511352692287744 1109 12 0.709383762401302 1109 13 0.630333186203849 1109 14 0.7126944636853025 1109 15 0.8078437617513927 1109 16 0.7811715398283283 1109 17 0.9438145121056678 1109 18 0.8747995071706853 1109 19 0.9259525308530527 1109 20 0.8256043271678593 1109 21 0.9029246080565443 1109 22 0.9066336005151427 1109 23 0.8329455846726717 1109 24 0.8874125075298167 1109 25 0.8432925043832142 1110 1 0.746912572755118 1110 2 3.4920552115164405 1110 3 1.8433346471052978 1110 4 0.8961439856741578 1110 5 0.5066070147736852 1110 6 0.2903921606734059 1110 7 0.094278540191155 1110 8 -0.03645214273025488 1110 9 0.05647208111501811 1110 10 -0.08014728622743661 1110 11 -0.08185715329360477 1110 12 -0.1449435149068421 1110 13 -0.0983460944557848 1110 14 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-0.5419158768142793 2315 12 -0.49712016098933276 2315 13 -0.5490377161759952 2315 14 -0.6034643562000923 2315 15 -1.0282675644578916 2315 16 -0.6375455501515361 2315 17 -0.6856283406123111 2315 18 -0.6008212661502554 2315 19 -0.6851220135690212 2315 20 -0.6018597299277957 2315 21 -0.6977155240997703 2315 22 -0.720089335833002 2315 23 -0.7565567641993525 2315 24 -0.6533184820938445 2315 25 -0.5277131225849345 2316 1 -0.2859194416339861 2316 2 0.7840704930771345 2316 3 0.36273179434135017 2316 4 -0.0896351010363432 2316 5 -0.5441367256045382 2316 6 -0.5221993373280114 2316 7 -0.5677344930716401 2316 8 -0.5828477436179043 2316 9 -0.7982089266029888 2316 10 -0.7880693612519423 2316 11 -0.9980093716428087 2316 12 -0.9045893135932204 2316 13 -0.7287748328517338 2316 14 -0.9725330370202698 2316 15 -0.799894764603053 2316 16 -1.0871293371230522 2316 17 -1.199838556544087 2316 18 -1.1484079110422911 2316 19 -0.9910329190308218 2316 20 -1.144042410022152 2316 21 -1.0812212399408168 2316 22 -0.9701800634686393 2316 23 -1.3634792827985152 2316 24 -1.238421004322113 2316 25 -1.0530714967457087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24710.0 3 1.0 4 202.0 5 905.0 6 1147.0 7 1035.0 8 894.0 9 934.0 10 894.0 11 851.0 12 757.0 13 794.0 14 782.0 15 832.0 16 886.0 17 945.0 18 1121.0 19 1356.0 20 1701.0 21 2098.0 22 2738.0 23 3037.0 24 3622.0 25 4221.0 26 4658.0 27 5467.0 28 6543.0 29 8152.0 30 10718.0 31 14323.0 32 19975.0 33 29298.0 34 44531.0 35 72659.0 36 130945.0 37 198559.0 38 547583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93885590943008 19.387950331949412 13.098652548018306 25.574541210602202 2 16.4079568387985 21.75186967079999 40.13282299016547 21.707350500236032 3 18.375665507699104 24.974997260569417 30.28801416503026 26.36132306670122 4 11.671800562496411 17.422778495730835 39.54172370190125 31.363697239871495 5 12.315175401826634 38.357767894569314 36.775159713151176 12.551896990452867 6 30.056423573365432 38.56448619587886 18.905984481778006 12.473105748977714 7 26.486206314778837 32.05716452411308 23.51657659882735 17.940052562280737 8 23.577887664213645 38.079737432101254 21.502790740550704 16.839584163134397 9 25.453310536632713 15.774250048266158 21.093789406491496 37.67865000860964 10 13.951963432515216 29.506450271942843 35.67547400845658 20.866112287085368 11 32.96909052644029 22.824326839288478 25.97997693660349 18.226605697667743 12 22.32157610312086 24.9022936426078 33.2048554885144 19.571274765756943 13 27.981413615752682 22.537773703901472 26.741973468397408 22.738839211948438 14 19.773644764556813 22.814586641666825 28.825853963129873 28.585914630646485 15 21.765863042385515 31.89514677260291 24.26587608729304 22.073114097718534 16 20.889680086687758 28.68279481056185 29.130669969057475 21.29685513369291 17 20.46885136980226 29.19215496654416 29.074142036431816 21.264851627221766 18 20.266952612780432 28.11377315811307 31.259441309299252 20.359832919807246 19 22.28781491959435 27.38723162967221 28.561812468854825 21.763140981878617 20 22.22769147281024 28.097347249194033 29.32540659191573 20.349554686079994 21 21.8311782283959 27.510897282028267 28.142470004209326 22.515454485366508 22 21.884487642311917 28.797557248721787 28.58196409795704 20.735991011009254 23 21.109618169247426 28.430193372207803 28.84233229407186 21.61785616447291 24 21.739742979864698 27.419075705013867 29.711419802743087 21.129761512378344 25 21.567276911155812 28.116816988399528 29.22494514582991 21.09096095461475 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 223.0 1 223.0 2 286.0 3 349.0 4 349.0 5 349.0 6 803.0 7 1257.0 8 1257.0 9 1257.0 10 1894.0 11 2531.0 12 2531.0 13 2531.0 14 4381.0 15 6231.0 16 6231.0 17 6231.0 18 11334.0 19 16437.0 20 16437.0 21 16437.0 22 25903.0 23 35369.0 24 35369.0 25 35369.0 26 50008.5 27 64648.0 28 64648.0 29 64648.0 30 83500.0 31 102352.0 32 102352.0 33 102352.0 34 113909.5 35 125467.0 36 125467.0 37 125467.0 38 132421.5 39 139376.0 40 139376.0 41 139376.0 42 144035.0 43 148694.0 44 148694.0 45 148694.0 46 147073.5 47 145453.0 48 145453.0 49 145453.0 50 136811.0 51 128169.0 52 128169.0 53 128169.0 54 111549.0 55 94929.0 56 94929.0 57 94929.0 58 80133.0 59 65337.0 60 65337.0 61 65337.0 62 52129.0 63 38921.0 64 38921.0 65 38921.0 66 29592.5 67 20264.0 68 20264.0 69 20264.0 70 14536.5 71 8809.0 72 8809.0 73 8809.0 74 6100.5 75 3392.0 76 3392.0 77 3392.0 78 2262.5 79 1133.0 80 1133.0 81 1133.0 82 751.0 83 369.0 84 369.0 85 369.0 86 242.5 87 116.0 88 116.0 89 116.0 90 74.5 91 33.0 92 33.0 93 33.0 94 22.5 95 12.0 96 12.0 97 12.0 98 7.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 2.9134496475265985 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 6.087623513532787E-4 19 0.001478422853286534 20 9.566265521265808E-4 21 0.004000438308892975 22 0.0020002191544464874 23 0.001652354953673185 24 2.608981505799766E-4 25 6.087623513532787E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1149874.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.04300476437625 #Duplication Level Percentage of deduplicated Percentage of total 1 87.42143449563706 68.2263142885161 2 9.142544844624624 14.27023341735126 3 1.956586225187627 4.580936042826926 4 0.6409754045881728 2.0009458621649103 5 0.26549974719947067 1.0360199017814489 6 0.15358261281860935 0.7191629150356849 7 0.0832823130723337 0.45497213691246885 8 0.05727139856651766 0.3575705624951383 9 0.04279223674766115 0.3005671262738432 >10 0.20249411140912488 2.8301794699508727 >50 0.01700932391131564 0.9189080639231111 >100 0.013941394613309637 2.21544892599491 >500 0.0016864510592412324 0.8827904258024233 >1k 8.994405649286574E-4 1.205950860970928 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2245 0.19523878268401582 No Hit GTCCTACAGTGGACATTTCTAAATT 2153 0.18723790606622986 No Hit CTGTAGGACGTGGAATATGGCAAGA 1938 0.16854020527466487 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1845 0.1604523626066856 No Hit CTTTAGGACGTGAAATATGGCGAGG 1667 0.14497240567227365 No Hit GGTATCAACGCAGAGTACTTTTTTT 1395 0.12131764001968912 No Hit TATCAACGCAGAGTACTTTTTTTTT 1324 0.11514305045596299 No Hit GTCCTACAGTGTGCATTTCTCATTT 1177 0.10235904107754415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 35 8.1073355E-5 19.510143 1 CCGGTTT 105 9.276846E-11 14.431796 12 CCAACGA 125 0.0 14.395716 19 GTTGCGG 40 0.005386636 14.206299 17 TCCAACG 165 0.0 13.775805 18 CGTACAC 50 0.0015342311 13.259212 3 GCCGGTT 115 3.947207E-10 13.176857 11 CGGTTTC 130 1.4551915E-11 13.113506 13 CTAGGGT 105 2.0785592E-8 12.627822 4 GTAGAAC 375 0.0 12.122708 3 TGCCGGT 125 1.4788384E-9 12.122708 10 GTACTAA 85 4.0602234E-5 11.476556 1 TAGGACC 380 0.0 11.464732 4 TAGAACA 435 0.0 11.321494 4 GGGTAAT 70 0.0011949299 11.148654 1 CTTACAC 130 3.4120603E-8 10.927922 3 GGTATCA 640 0.0 10.822033 1 CACCTTT 360 0.0 10.786264 14 GTAGGAC 1120 0.0 10.7392845 3 GAACAGT 275 0.0 10.676249 6 >>END_MODULE