FastQCFastQC Report
Thu 26 May 2016
SRR1511966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149874
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30270.2632462339351964No Hit
TATCAACGCAGAGTACTTTTTTTTT20700.18001972390018384No Hit
GGTATCAACGCAGAGTACTTTTTTT19670.1710622207302713No Hit
GTCCTACAGTGGACATTTCTAAATT19420.1688880694754382No Hit
CTGTAGGACGTGGAATATGGCAAGA19400.16871413737505153No Hit
CTTTAGGACGTGAAATATGGCGAGG17270.1501903686838732No Hit
GTCCTAAAGTGTGTATTTCTCATTT17070.14845104768000666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGA400.00529397314.2435099
TAGGACC4150.012.3654954
GTAACCC853.926387E-612.2981433
CTTATAC802.8552533E-511.8788893
GGTATCA9750.011.3532031
ACTTAGC600.00589403311.0782868
TTTATAC2100.010.86073
ATCCCGT1053.4819986E-610.85219810
GGCGAGG5450.010.630033519
CGTTTCC909.5532356E-510.55074814
CCAACGA1002.4043433E-510.44705719
TCCCGTT1002.4089637E-510.44524111
CTAGGAC1553.6343408E-910.4227683
GTTGTAA1303.6077108E-710.2766061
TCCAACG1303.8578764E-710.22655418
GGTATGA750.002550297210.1787331
GGGTTAC750.002550297210.1787331
TGTAGGA11600.010.1584982
AGGACCT8300.010.0755885
TAGATAG951.6375566E-410.0032755
Produced by FastQC (version 0.11.5)