FastQCFastQC Report
Thu 26 May 2016
SRR1511965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202490
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25120.2088998661111527No Hit
GGTATCAACGCAGAGTACTTTTTTT14720.12241266039634424No Hit
TATCAACGCAGAGTACTTTTTTTTT14320.11908622940731316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1052.8367685E-711.72308518
TAGGACC2600.011.6532344
GATTTGA853.9658655E-511.5031221
TTAGGAC4000.011.3619023
AGGACGT6700.011.3053765
TTATACT1105.183465E-711.1897534
GTGCTAC1156.075006E-711.05299951
CTTAGAC600.006022526411.0462943
TTTATAC1303.4266122E-810.9249073
GGACGTG7200.010.7832876
TAGAAAT2750.010.6733024
TATGAGT803.8877275E-410.6517844
TGTTACA1201.0512558E-610.5924592
GGTTTAG750.002083543510.4294971
GTCTAAT750.002083543510.4294971
GTAGGAC6950.010.3537723
GACGTGA3850.010.3290027
GGTATCA7200.010.3208561
GTAATAC1201.5889236E-610.2572743
CTCCTAT1157.2783714E-610.2027691
Produced by FastQC (version 0.11.5)