##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511965_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1202490 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30209565152309 31.0 30.0 34.0 25.0 34.0 2 27.400691897645718 31.0 27.0 34.0 10.0 34.0 3 29.361472444677293 31.0 28.0 34.0 16.0 34.0 4 33.89939874759873 37.0 35.0 37.0 28.0 37.0 5 34.347301848664024 37.0 35.0 37.0 32.0 37.0 6 34.64278705020416 37.0 35.0 37.0 32.0 37.0 7 34.735404868231754 37.0 35.0 37.0 32.0 37.0 8 34.78390672687507 37.0 35.0 37.0 32.0 37.0 9 36.50266696604545 39.0 37.0 39.0 33.0 39.0 10 36.42936905920216 39.0 37.0 39.0 33.0 39.0 11 36.450589194088934 39.0 37.0 39.0 33.0 39.0 12 36.36628163227969 39.0 37.0 39.0 32.0 39.0 13 36.365499920997266 39.0 37.0 39.0 32.0 39.0 14 37.682452244925116 40.0 38.0 41.0 33.0 41.0 15 37.631658475330354 40.0 38.0 41.0 33.0 41.0 16 37.584077206463256 40.0 38.0 41.0 32.0 41.0 17 37.55670234263902 40.0 38.0 41.0 33.0 41.0 18 37.51484419828855 40.0 38.0 41.0 32.0 41.0 19 37.5364368934461 40.0 38.0 41.0 32.0 41.0 20 37.45738592420727 40.0 38.0 41.0 32.0 41.0 21 37.410573892506385 40.0 38.0 41.0 32.0 41.0 22 37.327024756962636 40.0 38.0 41.0 32.0 41.0 23 37.248563397616614 40.0 38.0 41.0 32.0 41.0 24 37.176276725793976 40.0 38.0 41.0 32.0 41.0 25 37.08725727448877 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8237336462488898 1101 2 3.9274766361897555 1101 3 1.9861484446174238 1101 4 0.9841224172831531 1101 5 0.6235960637887246 1101 6 0.3481592193522758 1101 7 0.09150809033594953 1101 8 0.09966281122483167 1101 9 0.01544068192414727 1101 10 0.06557840480879662 1101 11 0.2204865414347239 1101 12 0.16111529710592976 1101 13 0.17044733511902166 1101 14 0.12042858631956221 1101 15 0.11358498211740908 1101 16 0.22246572437562406 1101 17 0.3372723703997238 1101 18 0.29777094797488246 1101 19 0.203380019985822 1101 20 0.33175449688444303 1101 21 0.1398514467909706 1101 22 0.3052201747956005 1101 23 0.26925021421864415 1101 24 0.05724390484834885 1101 25 -0.04336085907846865 1102 1 1.479851950386994 1102 2 4.3880376922953666 1102 3 2.512568009650849 1102 4 1.5428640606782622 1102 5 0.9818003457553814 1102 6 0.8582040699911886 1102 7 0.7518660492087577 1102 8 0.7032297063758861 1102 9 0.6929730505456249 1102 10 0.3760717618368119 1102 11 0.6673808462498201 1102 12 0.6555089672421701 1102 13 0.6256770888636254 1102 14 0.8072526533416493 1102 15 0.6532016360904933 1102 16 0.7939263319748449 1102 17 0.6357025980378737 1102 18 0.7681864510636487 1102 19 0.6927258776485132 1102 20 0.572479723253231 1102 21 0.707262803311238 1102 22 0.7093709744909518 1102 23 0.5948573903208683 1102 24 0.6420369995424338 1102 25 0.631400078553753 1103 1 1.2577286834697325 1103 2 4.030371590697616 1103 3 2.170791400228122 1103 4 1.1999757395880906 1103 5 0.9189917075714646 1103 6 0.796656600107724 1103 7 0.4727322425278473 1103 8 0.5779092959229359 1103 9 0.5802049499142257 1103 10 0.5236980629284531 1103 11 0.6239053448535259 1103 12 0.40746214022696137 1103 13 0.6505135055077744 1103 14 0.6703872298265878 1103 15 0.7358347753349435 1103 16 0.4665241748856843 1103 17 0.5105009339508655 1103 18 0.5555321831554707 1103 19 0.4203913240938988 1103 20 0.5609797519161503 1103 21 0.6074830982935921 1103 22 0.5216800589974184 1103 23 0.6706011929889755 1103 24 0.8301967584463625 1103 25 0.8540474673621148 1104 1 1.3756692106844532 1104 2 4.110268843981963 1104 3 2.39924507021405 1104 4 1.5902232608839952 1104 5 1.0872421399348013 1104 6 0.8260660347590942 1104 7 0.5366213104491706 1104 8 0.6862512228132474 1104 9 0.7372730162564807 1104 10 0.7441442833746805 1104 11 0.5440685150166971 1104 12 0.7117244102150408 1104 13 0.762705077376765 1104 14 0.7454036112945843 1104 15 0.7733127543451772 1104 16 0.8184350328449312 1104 17 0.9155662986936477 1104 18 0.7852265479304776 1104 19 0.8047522588580591 1104 20 0.7115677391976831 1104 21 0.5494363063758314 1104 22 0.7470783166537416 1104 23 0.8755702830387122 1104 24 0.8842710443754882 1104 25 0.8947621290445156 1105 1 1.549496772012013 1105 2 4.477476636189756 1105 3 2.661240019709002 1105 4 1.6855876187483574 1105 5 1.2714593116519737 1105 6 1.0753264965195513 1105 7 0.8531076019354629 1105 8 0.7049741665361893 1105 9 0.7639144303978966 1105 10 0.7255295647599596 1105 11 0.7205475914957731 1105 12 0.8112373972280267 1105 13 0.8451726098442975 1105 14 0.870367536258513 1105 15 0.8163322348646602 1105 16 0.8727099246198264 1105 17 0.9419732251005755 1105 18 1.0099199101238412 1105 19 1.0268842934900988 1105 20 0.9252221403520835 1105 21 0.8359442428837696 1105 22 0.8940480136234399 1105 23 0.8915334865019133 1105 24 0.9795271771316223 1105 25 0.9576769919593815 1106 1 0.16301485684157413 1106 2 2.335673357501232 1106 3 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15 0.6616831714478266 1107 16 0.6927618316385562 1107 17 0.7823365742316994 1107 18 0.7048232427048973 1107 19 0.7689853993352784 1107 20 0.8183425796810582 1107 21 0.6823069901943839 1107 22 0.6868825882115672 1107 23 0.7975930667321123 1107 24 0.8997521075461208 1107 25 0.9155608406015219 1108 1 1.1169570894723329 1108 2 3.3687464774595988 1108 3 1.9564781149470925 1108 4 1.2260638092245486 1108 5 0.8119355021281649 1108 6 0.6729455441385994 1108 7 0.44040918923705163 1108 8 0.4986249601869872 1108 9 0.5655017319852007 1108 10 0.5199740092044038 1108 11 0.41499203594021594 1108 12 0.44139612738675993 1108 13 0.4102519749236606 1108 14 0.33465325054422124 1108 15 0.45839572692815267 1108 16 0.4838210357309336 1108 17 0.5649890981164489 1108 18 0.549602449806379 1108 19 0.6070430236488278 1108 20 0.614904680034627 1108 21 0.6748331317726581 1108 22 0.5765876961631236 1108 23 0.6367715817400068 1108 24 0.5541303517347913 1108 25 0.519581753864145 1109 1 0.8632949490466402 1109 2 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2313 25 0.7043428991516052 2314 1 0.17523866297129587 2314 2 1.897437110498057 2314 3 0.9913611063518246 2314 4 0.3447757735886867 2314 5 -0.03859778101381295 2314 6 -0.041459374614142064 2314 7 -0.30898851450847786 2314 8 -0.23533262811842803 2314 9 -0.21826763874069144 2314 10 -0.2609375912724161 2314 11 -0.23913306601097162 2314 12 -0.3310268602536226 2314 13 -0.37312213888523615 2314 14 -0.36509892964274115 2314 15 -0.48826968495465195 2314 16 -0.6097764296056596 2314 17 -0.4908989751629278 2314 18 -0.5187048467617856 2314 19 -0.31455180664165283 2314 20 -0.43519256571479303 2314 21 -0.40220118656601045 2314 22 -0.5139041791113641 2314 23 -0.572319327350904 2314 24 -0.42587153044100035 2314 25 -0.5698059116104801 2315 1 -2.6490251265098834 2315 2 -11.375881267168147 2315 3 -4.780942422473444 2315 4 -1.9299811968204565 2315 5 -1.122370882178224 2315 6 -0.6870599158668611 2315 7 -0.5819436831158242 2315 8 -0.6059126943506712 2315 9 -0.8262653597818925 2315 10 -0.8093832701528783 2315 11 -0.83492411397593 2315 12 -0.7215050855144582 2315 13 -0.8313922667205773 2315 14 -0.9835656176746426 2315 15 -1.058272889740465 2315 16 -1.0213991194892174 2315 17 -0.9735468404194876 2315 18 -1.0178110676071341 2315 19 -1.1199216033158024 2315 20 -0.9945060293760832 2315 21 -1.0725070655675424 2315 22 -1.051681482106055 2315 23 -0.9470941021256749 2315 24 -0.7702934226889795 2315 25 -0.9267834425010548 2316 1 -0.11181143780612857 2316 2 0.40909915274604813 2316 3 0.3533981191518656 2316 4 0.11234311123232743 2316 5 -0.23805135795803523 2316 6 -0.37762472812040926 2316 7 -0.30687873255418197 2316 8 -0.4366846161821982 2316 9 -0.48179144577155597 2316 10 -0.6087734942119738 2316 11 -0.6318044530748139 2316 12 -0.6225137853642124 2316 13 -0.8113789511774598 2316 14 -0.6927145619229265 2316 15 -0.581728313858143 2316 16 -0.9125628599907074 2316 17 -0.8693954283210559 2316 18 -0.8930707342575275 2316 19 -0.7071112914988689 2316 20 -0.7328773025155684 2316 21 -0.8519355006251175 2316 22 -0.8344130396720502 2316 23 -0.7176986169354862 2316 24 -0.8154849115890812 2316 25 -0.7469876774403872 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26259.0 3 1.0 4 232.0 5 1039.0 6 1190.0 7 1097.0 8 1038.0 9 992.0 10 1003.0 11 875.0 12 915.0 13 925.0 14 887.0 15 909.0 16 1045.0 17 1080.0 18 1330.0 19 1576.0 20 2032.0 21 2584.0 22 3198.0 23 3610.0 24 4333.0 25 4929.0 26 5616.0 27 6696.0 28 8125.0 29 10115.0 30 12946.0 31 17518.0 32 24207.0 33 34643.0 34 52283.0 35 82414.0 36 141422.0 37 207013.0 38 536413.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41973737827342 18.12397608296119 12.860481168242563 25.595805370522832 2 17.576726738074463 20.987605548646847 38.07360407835162 23.36206363492707 3 18.957080724164026 23.63587223178571 29.667772704970517 27.739274339079746 4 12.878942860231685 16.346248201648248 37.2429708355163 33.531838102603764 5 13.976665086611947 36.936440219877085 35.141664379745364 13.945230313765602 6 32.58804647024092 36.0660795515971 17.867009289058537 13.478864689103442 7 28.40672271702883 31.41972074611847 21.602175485866827 18.57138105098587 8 25.932711234780527 35.55209236841252 20.25781524820601 18.257381148600942 9 26.533526266330696 15.560462041264374 19.60257465758551 38.30343703481942 10 15.544175456074857 28.306371201732404 33.54134632416596 22.608107018026775 11 34.598653293294156 22.259746243000976 24.018182286906576 19.12341817679829 12 23.668887059351846 23.992299312260393 30.870942793703065 21.467870834684696 13 28.79175710400918 21.308784272634284 25.71672113697411 24.182737486382422 14 21.735500283162672 21.05349820247836 27.091557594289846 30.119443920069124 15 23.540154180076343 29.092300143868137 23.514457500686074 23.853088175369443 16 23.16227161972241 27.307170953604604 26.454357208791755 23.07620021788123 17 22.374156957646218 27.487962477858446 27.176026411862054 22.961854152633286 18 22.33762194518527 26.852930634593143 28.571784975637993 22.2376624445836 19 23.899175457490117 26.39375958951671 26.98545096196626 22.721613991026913 20 23.913791927723064 26.528957509692418 27.387761687038353 22.169488875546158 21 23.693844285880612 26.08361192887913 26.8362357167806 23.38630806845966 22 23.72421145773041 26.611264129227123 26.940253078657967 22.7242713343845 23 23.31621600269442 26.397834484421846 27.094225707597186 23.19172380528655 24 23.459155585493434 26.19140283910885 27.3629718334456 22.986469741952114 25 23.463748940738753 26.30859824913159 27.31858669809038 22.909066112039277 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 451.0 1 451.0 2 434.0 3 417.0 4 417.0 5 417.0 6 836.5 7 1256.0 8 1256.0 9 1256.0 10 1679.5 11 2103.0 12 2103.0 13 2103.0 14 3393.5 15 4684.0 16 4684.0 17 4684.0 18 8120.0 19 11556.0 20 11556.0 21 11556.0 22 18034.5 23 24513.0 24 24513.0 25 24513.0 26 35840.5 27 47168.0 28 47168.0 29 47168.0 30 61531.0 31 75894.0 32 75894.0 33 75894.0 34 87196.0 35 98498.0 36 98498.0 37 98498.0 38 109537.5 39 120577.0 40 120577.0 41 120577.0 42 132544.0 43 144511.0 44 144511.0 45 144511.0 46 150742.5 47 156974.0 48 156974.0 49 156974.0 50 157000.0 51 157026.0 52 157026.0 53 157026.0 54 145036.0 55 133046.0 56 133046.0 57 133046.0 58 115909.5 59 98773.0 60 98773.0 61 98773.0 62 81384.0 63 63995.0 64 63995.0 65 63995.0 66 49917.0 67 35839.0 68 35839.0 69 35839.0 70 25941.5 71 16044.0 72 16044.0 73 16044.0 74 11064.5 75 6085.0 76 6085.0 77 6085.0 78 4105.0 79 2125.0 80 2125.0 81 2125.0 82 1400.0 83 675.0 84 675.0 85 675.0 86 435.5 87 196.0 88 196.0 89 196.0 90 131.5 91 67.0 92 67.0 93 67.0 94 39.5 95 12.0 96 12.0 97 12.0 98 8.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.168259195502665 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 8.316077472577734E-5 9 0.0 10 8.316077472577734E-5 11 8.316077472577734E-5 12 0.0 13 0.0 14 8.316077472577734E-5 15 0.0 16 0.0 17 0.0 18 2.4948232417733204E-4 19 4.1580387362888675E-4 20 3.3264309890310937E-4 21 0.0024948232417733203 22 0.0014968939450639921 23 4.1580387362888675E-4 24 0.0 25 8.316077472577734E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1202490.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.09260140562112 #Duplication Level Percentage of deduplicated Percentage of total 1 79.75394231558694 55.90161289253336 2 13.259407826388905 18.587727752993015 3 3.777657184510397 7.943574578429047 4 1.412264729568933 3.959572350755701 5 0.6746921264043522 2.3645463143785603 6 0.3480284466332907 1.463653151261084 7 0.20491402560075234 1.0054069983198344 8 0.11960771965184305 0.670689297487353 9 0.07885906870912443 0.4974693543222434 >10 0.33998499296549145 3.9699484273546313 >50 0.01780277916073803 0.8583008664969022 >100 0.01103896410291915 1.6023303730730138 >500 0.0013198685253300577 0.6257244157237801 >1k 4.79952191029112E-4 0.5494432268714322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2512 0.2088998661111527 No Hit GGTATCAACGCAGAGTACTTTTTTT 1472 0.12241266039634424 No Hit TATCAACGCAGAGTACTTTTTTTTT 1432 0.11908622940731316 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.316077472577734E-5 0.0 10 0.0 0.0 0.0 8.316077472577734E-5 0.0 11 0.0 0.0 0.0 8.316077472577734E-5 0.0 12 0.0 0.0 0.0 8.316077472577734E-5 0.0 13 0.0 0.0 0.0 8.316077472577734E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 105 2.8367685E-7 11.723085 18 TAGGACC 260 0.0 11.653234 4 GATTTGA 85 3.9658655E-5 11.503122 1 TTAGGAC 400 0.0 11.361902 3 AGGACGT 670 0.0 11.305376 5 TTATACT 110 5.183465E-7 11.189753 4 GTGCTAC 115 6.075006E-7 11.0529995 1 CTTAGAC 60 0.0060225264 11.046294 3 TTTATAC 130 3.4266122E-8 10.924907 3 GGACGTG 720 0.0 10.783287 6 TAGAAAT 275 0.0 10.673302 4 TATGAGT 80 3.8877275E-4 10.651784 4 TGTTACA 120 1.0512558E-6 10.592459 2 GGTTTAG 75 0.0020835435 10.429497 1 GTCTAAT 75 0.0020835435 10.429497 1 GTAGGAC 695 0.0 10.353772 3 GACGTGA 385 0.0 10.329002 7 GGTATCA 720 0.0 10.320856 1 GTAATAC 120 1.5889236E-6 10.257274 3 CTCCTAT 115 7.2783714E-6 10.202769 1 >>END_MODULE