FastQCFastQC Report
Thu 26 May 2016
SRR1511965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202490
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32530.2705220001829537No Hit
GGTATCAACGCAGAGTACTTTTTTT20890.17372285840214888No Hit
TATCAACGCAGAGTACTTTTTTTTT20440.1699806235394889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT1650.013.2991191
TCGAACT655.468652E-513.14918119
TAGGACT1001.918108E-611.4049684
GCGTGCG1001.9393447E-611.3945369
AGGACGT6550.011.3179095
GGTATCA8900.011.1486511
GGACGTG6450.011.0476476
GCGCCAC909.5563766E-510.55049613
TATGAGT1106.0090315E-610.3681544
TAGGACG7100.010.3073074
GGCGTGC1201.5315345E-610.2867358
TTAGGAC4350.010.2688433
GACGTGA3900.010.2258667
AGGCGTG1303.8620783E-710.2258667
GGCGAGG3350.010.20533519
GTACTAA750.002554638310.1767181
TGCGCCA1054.1237716E-59.94761112
GCCTCGA1054.1237716E-59.94761116
TGTAGGA6550.09.7217942
GTAGGAC6600.09.6481443
Produced by FastQC (version 0.11.5)