##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511965_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1202490 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.995819507854534 31.0 30.0 34.0 26.0 34.0 2 30.192153780904622 31.0 30.0 34.0 26.0 34.0 3 30.26590740879342 31.0 30.0 34.0 26.0 34.0 4 33.86408452461143 35.0 35.0 37.0 30.0 37.0 5 33.43303395454432 35.0 35.0 37.0 28.0 37.0 6 33.378398157157235 36.0 35.0 37.0 28.0 37.0 7 33.17568545268568 36.0 35.0 37.0 27.0 37.0 8 33.04895508486557 35.0 33.0 37.0 26.0 37.0 9 34.39898377533285 38.0 35.0 39.0 27.0 39.0 10 34.133210255386736 37.0 34.0 39.0 27.0 39.0 11 34.16190488070587 38.0 34.0 39.0 26.0 39.0 12 33.921818060857056 37.0 34.0 39.0 25.0 39.0 13 33.89655548071086 37.0 34.0 39.0 25.0 39.0 14 34.699917670833024 38.0 34.0 40.0 25.0 41.0 15 34.587248958411294 38.0 34.0 40.0 25.0 41.0 16 34.443710966411366 38.0 33.0 40.0 24.0 41.0 17 34.333622732829376 38.0 33.0 40.0 23.0 41.0 18 34.211190945454845 38.0 33.0 40.0 22.0 41.0 19 34.222860896972115 38.0 34.0 40.0 22.0 41.0 20 34.05381001089406 38.0 33.0 40.0 19.0 41.0 21 33.90517675822668 38.0 33.0 40.0 18.0 41.0 22 33.741651905629155 38.0 33.0 40.0 18.0 41.0 23 33.81478764896174 38.0 34.0 40.0 18.0 41.0 24 33.64076707498607 38.0 33.0 40.0 16.0 41.0 25 33.059810060790525 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.007523757836999323 1101 2 0.023033645702962957 1101 3 0.0887629191649566 1101 4 -0.027951568030232465 1101 5 -0.10760685358991395 1101 6 0.06008195706792918 1101 7 -0.2653956756088647 1101 8 -0.11409121739188066 1101 9 -0.2489014835968817 1101 10 -0.37380209735663783 1101 11 -0.2515247835227896 1101 12 -0.27732713392332187 1101 13 -0.4590272763426029 1101 14 -0.29697422793827144 1101 15 -0.3971871308920356 1101 16 -0.19534513390229336 1101 17 -0.37544722069109326 1101 18 -0.183803547366594 1101 19 -0.05814109249757138 1101 20 -0.16018579346553707 1101 21 -0.23704726739484983 1101 22 -0.11231739200729152 1101 23 -0.6280429011735507 1101 24 -0.6080184854480635 1101 25 -0.7028279674406619 1102 1 0.4143853561513211 1102 2 0.4294904452290673 1102 3 0.36471282184753306 1102 4 0.5020391233465986 1102 5 0.6328094957373267 1102 6 0.6389090705485074 1102 7 0.5765819836955259 1102 8 0.5782460932622584 1102 9 0.758608882824376 1102 10 0.7308733701901176 1102 11 0.5151672702979795 1102 12 0.872837882578331 1102 13 0.9314186913310891 1102 14 0.8369980154399173 1102 15 0.6682482588007801 1102 16 0.8234625160934783 1102 17 0.9208462548103 1102 18 0.7570147652338974 1102 19 1.042425887277325 1102 20 1.0493386205143267 1102 21 1.133465553117972 1102 22 1.1537399829609711 1102 23 1.1039406818001254 1102 24 1.2075057592841034 1102 25 1.1245139975250638 1103 1 0.2426003116419082 1103 2 0.23921781995165148 1103 3 0.2679468177036952 1103 4 0.3467106872771879 1103 5 0.39652879570702737 1103 6 0.45214151041780326 1103 7 0.3585083773274462 1103 8 0.473198553768853 1103 9 0.4679443945393231 1103 10 0.49735864154603604 1103 11 0.603727491084328 1103 12 0.747486761858319 1103 13 0.733390699946348 1103 14 0.5637646747361202 1103 15 0.633416673905316 1103 16 0.8267116623480533 1103 17 1.0018067081757351 1103 18 0.521297086766296 1103 19 0.44844837538221327 1103 20 0.4905448379102566 1103 21 0.6373944199500627 1103 22 0.4124965037743422 1103 23 0.6183315129427953 1103 24 0.8110055013178581 1103 25 1.061357309647839 1104 1 0.4470591592459172 1104 2 0.6607653584346735 1104 3 0.6403627082647461 1104 4 0.6432300281513648 1104 5 0.5473839889009326 1104 6 0.749038833524807 1104 7 0.5801046573914732 1104 8 0.7589954181947505 1104 9 0.6823825101871108 1104 10 0.5678284942739538 1104 11 0.8521840076860059 1104 12 0.9797074981113099 1104 13 0.852500085184765 1104 14 1.2135624575984139 1104 15 1.036004677299772 1104 16 1.1991215105643533 1104 17 1.2318246740807979 1104 18 1.209031395468351 1104 19 1.2397679429114632 1104 20 0.965094481814738 1104 21 1.248184167836584 1104 22 1.1083577661678632 1104 23 0.9567625711319181 1104 24 0.8466768192472429 1104 25 0.6762263116136182 1105 1 0.35804400773076495 1105 2 0.4680885907579082 1105 3 0.32807915848119507 1105 4 0.45098616090749744 1105 5 0.33012208413902755 1105 6 0.5260770730630426 1105 7 0.46183265161946707 1105 8 0.5870076837070215 1105 9 0.7011595664641703 1105 10 0.646832823278281 1105 11 0.9142871822891792 1105 12 0.5694982629020799 1105 13 0.666064298748978 1105 14 0.7865422555782189 1105 15 1.0125198288149235 1105 16 0.9934216548644983 1105 17 0.7779105326666595 1105 18 0.6536201400570931 1105 19 0.8185377841813022 1105 20 0.9710107877310463 1105 21 1.0771163467687614 1105 22 1.0518462221563212 1105 23 0.8782452551145994 1105 24 1.1661573387277642 1105 25 0.6504687358560375 1106 1 -0.2835641109265339 1106 2 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-1.1197286517266534 2315 12 -1.3182771748733586 2315 13 -1.247739765055087 2315 14 -1.6797773107413505 2315 15 -1.6492503829552874 2315 16 -1.9519548905120487 2315 17 -1.880663625907502 2315 18 -1.7327902963533433 2315 19 -1.39544762980411 2315 20 -1.5888718221515603 2315 21 -1.6509324812800656 2315 22 -1.8384876181775205 2315 23 -1.528078601209252 2315 24 -1.773570050999627 2315 25 -1.8352981999108948 2316 1 -1.4765507573275762 2316 2 -1.190862318524129 2316 3 -1.3764462278322718 2316 4 -1.5015945181632446 2316 5 -1.5034083479013383 2316 6 -1.554580973820169 2316 7 -1.683242508687485 2316 8 -1.7606087359292673 2316 9 -2.338425737856234 2316 10 -2.4093797279673836 2316 11 -2.382383163553353 2316 12 -2.5093911515833653 2316 13 -2.3008283744350884 2316 14 -2.5759038707751927 2316 15 -2.453287487489085 2316 16 -2.3725328284740925 2316 17 -2.680002323425004 2316 18 -2.4507475672907475 2316 19 -2.4971644128122854 2316 20 -2.6149347604661557 2316 21 -2.292583012003444 2316 22 -2.2139900019580487 2316 23 -2.440873319467549 2316 24 -2.4965437019534136 2316 25 -2.5883518254810767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32100.0 3 2.0 4 546.0 5 4232.0 6 5448.0 7 4373.0 8 3664.0 9 3269.0 10 3086.0 11 2966.0 12 2694.0 13 2748.0 14 2731.0 15 2630.0 16 2770.0 17 2819.0 18 3065.0 19 3418.0 20 4080.0 21 5198.0 22 6571.0 23 8085.0 24 9649.0 25 11375.0 26 12943.0 27 15007.0 28 17315.0 29 20674.0 30 26432.0 31 33727.0 32 43585.0 33 56457.0 34 74799.0 35 103666.0 36 156968.0 37 251037.0 38 262361.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.11835597312118 19.924711258155376 14.216845939493439 23.740086829230002 2 16.561551447413283 21.68658367221349 38.299694799956754 23.45217008041647 3 20.082994453176326 25.101414564778086 30.077006877396066 24.73858410464952 4 14.53966353150546 18.112250413724855 36.30283827724139 31.045247777528296 5 14.10797874066119 37.74447698915321 34.87543122201268 13.272113048172917 6 32.56253161523921 37.1537299326429 17.25085527541865 13.032883176699237 7 28.55000873188135 31.80866368951093 21.530158254954305 18.11116932365342 8 26.262754783823567 36.10749361741054 19.887400311021295 17.742351287744597 9 26.89860206737686 15.419255045780005 19.400992939650223 38.28114994719291 10 15.659839167061682 28.981613152708128 33.3754958461193 21.983051834110885 11 35.28320401832863 22.37299270680006 23.63978078819782 18.704022486673484 12 23.78556162629211 24.607106919808064 30.749943866477057 20.857387587422764 13 29.320077505842047 21.510615472893747 25.616013438781195 23.553293582483015 14 22.08558906934777 21.34728771133232 26.923882942893496 29.643240276426415 15 23.918888239310295 29.58039533001965 23.241376844195663 23.259339586474386 16 23.46555896514732 27.79499205814601 26.11921928664687 22.620229690059794 17 22.704887358730634 27.904847441558765 26.817936115892856 22.572329083817742 18 22.639439829021445 27.209872847175443 28.29137872248418 21.85930860131893 19 24.298996249449058 26.65735265989738 26.924714550640754 22.118936540012808 20 24.327560857156165 26.79810526175729 27.101226789789166 21.773107091297376 21 24.04710226280468 26.201298971301217 26.720471687914245 23.031127077979857 22 24.23163602192118 26.870909529393177 26.7129040574142 22.184550391271447 23 23.689926735357467 26.69402656155145 26.80113763939825 22.814909063692838 24 23.820402257030352 26.473618774766834 27.168025680581138 22.537953287621676 25 23.819060704554754 26.703832833755285 27.149377717599233 22.32772874409073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 522.0 1 522.0 2 444.0 3 366.0 4 366.0 5 366.0 6 828.5 7 1291.0 8 1291.0 9 1291.0 10 1787.5 11 2284.0 12 2284.0 13 2284.0 14 3664.0 15 5044.0 16 5044.0 17 5044.0 18 8635.0 19 12226.0 20 12226.0 21 12226.0 22 19204.0 23 26182.0 24 26182.0 25 26182.0 26 37854.0 27 49526.0 28 49526.0 29 49526.0 30 64295.5 31 79065.0 32 79065.0 33 79065.0 34 89948.0 35 100831.0 36 100831.0 37 100831.0 38 112406.0 39 123981.0 40 123981.0 41 123981.0 42 134962.0 43 145943.0 44 145943.0 45 145943.0 46 151248.5 47 156554.0 48 156554.0 49 156554.0 50 155425.0 51 154296.0 52 154296.0 53 154296.0 54 141854.0 55 129412.0 56 129412.0 57 129412.0 58 112587.0 59 95762.0 60 95762.0 61 95762.0 62 78503.5 63 61245.0 64 61245.0 65 61245.0 66 47750.0 67 34255.0 68 34255.0 69 34255.0 70 24709.0 71 15163.0 72 15163.0 73 15163.0 74 10459.0 75 5755.0 76 5755.0 77 5755.0 78 3891.0 79 2027.0 80 2027.0 81 2027.0 82 1310.0 83 593.0 84 593.0 85 593.0 86 364.5 87 136.0 88 136.0 89 136.0 90 81.5 91 27.0 92 27.0 93 27.0 94 14.0 95 1.0 96 1.0 97 1.0 98 2.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4130595680629361 2 0.0 3 0.0 4 0.0 5 0.03185057671997272 6 0.04474049680246821 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 8.316077472577734E-5 16 0.0 17 0.0 18 0.0 19 0.0 20 1.6632154945155468E-4 21 0.0 22 0.0 23 0.0 24 0.0020790193681444334 25 0.011808830011060383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1202490.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.07664708086782 #Duplication Level Percentage of deduplicated Percentage of total 1 80.64846557380919 56.51574059629344 2 12.489158031868298 17.50396639472841 3 3.6653773875931517 7.705720728256756 4 1.399272717705634 3.9222536163417794 5 0.6363372730139657 2.2296191252700757 6 0.35411669152691344 1.4889186250545627 7 0.2072220092507026 1.0164996526754866 8 0.12927117476525524 0.7247112393403181 9 0.08661339426913751 0.5462618636406967 >10 0.35093240529246145 4.151144020419827 >50 0.019960739036085952 0.965321764162554 >100 0.011139515688252607 1.666867825480952 >500 0.0014220574539718866 0.6969067748593991 >1k 7.110287269859433E-4 0.8660677734757714 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3253 0.2705220001829537 No Hit GGTATCAACGCAGAGTACTTTTTTT 2089 0.17372285840214888 No Hit TATCAACGCAGAGTACTTTTTTTTT 2044 0.1699806235394889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 8.316077472577734E-5 13 0.0 0.0 0.0 0.0 8.316077472577734E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAT 165 0.0 13.299119 1 TCGAACT 65 5.468652E-5 13.149181 19 TAGGACT 100 1.918108E-6 11.404968 4 GCGTGCG 100 1.9393447E-6 11.394536 9 AGGACGT 655 0.0 11.317909 5 GGTATCA 890 0.0 11.148651 1 GGACGTG 645 0.0 11.047647 6 GCGCCAC 90 9.5563766E-5 10.550496 13 TATGAGT 110 6.0090315E-6 10.368154 4 TAGGACG 710 0.0 10.307307 4 GGCGTGC 120 1.5315345E-6 10.286735 8 TTAGGAC 435 0.0 10.268843 3 GACGTGA 390 0.0 10.225866 7 AGGCGTG 130 3.8620783E-7 10.225866 7 GGCGAGG 335 0.0 10.205335 19 GTACTAA 75 0.0025546383 10.176718 1 TGCGCCA 105 4.1237716E-5 9.947611 12 GCCTCGA 105 4.1237716E-5 9.947611 16 TGTAGGA 655 0.0 9.721794 2 GTAGGAC 660 0.0 9.648144 3 >>END_MODULE