FastQCFastQC Report
Thu 26 May 2016
SRR1511964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006744
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT30740.30534078176775825No Hit
GTCCTACAGTGGACATTTCTAAATT30070.29868566388277457No Hit
CTGTAGGACGTGGAATATGGCAAGA29750.2955071001168122No Hit
CTTTAGGACGTGAAATATGGCGAGG29000.28805734129033794No Hit
GTATCAACGCAGAGTACTTTTTTTT23930.237696971623372No Hit
TATCAACGCAGAGTACTTTTTTTTT15280.15177641982470222No Hit
GGTATCAACGCAGAGTACTTTTTTT14940.14839919582336722No Hit
CTGAAGGACCTGGAATATGGCGAGA14390.1429360393506194No Hit
GTCCTACAGTGTGCATTTCTCATTT12760.12674523016774872No Hit
TTTCTAAATTTTCCACCTTTTTCAG12370.12287135557798208No Hit
ATTTAGAAATGTCCACTGTAGGACG11860.11780551957597958No Hit
GAATATGGCAAGAAAACTGAAAATC10810.10737585721891563No Hit
CTGTAGGACCTGGAATATGGCGAGA10730.10658121627742505No Hit
GGAATATGGCGAGAAAACTGAAAAT10720.1064818861597387No Hit
GTCCTTCAGTGTGCATTTCTCATTT10180.10111805980467727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG350.001802222616.7438811
GGCGAAA402.8275783E-416.5722819
TGGCGAA508.948195E-515.15180118
CGGTAGG400.004389473714.6508961
TCCAACG1500.013.25782618
TAGGACC3950.013.1852474
CCAACGT655.6135486E-513.11213519
GCTTAGA708.4116895E-512.5579112
CCAACGA1001.5012665E-712.31083819
GTATTAG1051.8939863E-712.0928031
GGCCTAC1051.8939863E-712.0928031
AAATGTC4350.011.9728127
TGCCGGT1157.384551E-811.52797110
GACGTGA9700.011.5194847
AGGACGT19200.011.4421985
GGACGTG19050.011.4328786
ACTGATC752.1354483E-411.3632878
GGTATCA5750.011.2111221
ACGTGAA10900.011.1200348
ACCTTTT4950.011.09546515
Produced by FastQC (version 0.11.5)