##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511964_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1006744 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.533483189370884 31.0 31.0 34.0 26.0 34.0 2 27.68058712045962 31.0 27.0 34.0 10.0 34.0 3 29.622632963295533 31.0 30.0 34.0 16.0 34.0 4 34.11229369134556 37.0 35.0 37.0 31.0 37.0 5 34.56600188329903 37.0 35.0 37.0 32.0 37.0 6 34.85982235801753 37.0 35.0 37.0 32.0 37.0 7 34.95924981921919 37.0 35.0 37.0 33.0 37.0 8 34.99789122160152 37.0 35.0 37.0 33.0 37.0 9 36.75102806671806 39.0 37.0 39.0 34.0 39.0 10 36.67942595138387 39.0 37.0 39.0 33.0 39.0 11 36.686700889203216 39.0 37.0 39.0 33.0 39.0 12 36.63099457260237 39.0 37.0 39.0 33.0 39.0 13 36.63574453883013 39.0 37.0 39.0 33.0 39.0 14 37.99434811630365 40.0 38.0 41.0 34.0 41.0 15 37.96077850973038 40.0 38.0 41.0 33.0 41.0 16 37.91796822230875 40.0 38.0 41.0 33.0 41.0 17 37.90974368856432 40.0 38.0 41.0 33.0 41.0 18 37.88074724060933 40.0 38.0 41.0 33.0 41.0 19 37.91789074481695 40.0 39.0 41.0 34.0 41.0 20 37.851987198334434 40.0 38.0 41.0 34.0 41.0 21 37.80706217270726 40.0 38.0 41.0 34.0 41.0 22 37.73094848342776 40.0 38.0 41.0 33.0 41.0 23 37.65948940346305 40.0 38.0 41.0 33.0 41.0 24 37.61941764738603 40.0 38.0 41.0 33.0 41.0 25 37.54720862503278 40.0 38.0 41.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3124820131866208 1101 2 4.374129188143584 1101 3 2.509317481096197 1101 4 1.477451215151575 1101 5 1.0437814067272768 1101 6 0.7507095015237297 1101 7 0.3149961419977103 1101 8 0.2658271396588532 1101 9 0.3226583552902227 1101 10 0.4724513534661128 1101 11 0.589048394996496 1101 12 0.5361366323688515 1101 13 0.602280147450557 1101 14 0.3280097774261037 1101 15 0.47533353999529737 1101 16 0.5867228180304807 1101 17 0.6268508973263138 1101 18 0.5894418076541257 1101 19 0.5045518964610736 1101 20 0.5974787994388038 1101 21 0.5705501479823241 1101 22 0.5133839318539088 1101 23 0.6365104760000122 1101 24 0.6076444020345733 1101 25 0.43358324539084236 1102 1 1.4439177846452367 1102 2 4.319251880022438 1102 3 2.5311019748732235 1102 4 1.5271615952380486 1102 5 1.0744974341754485 1102 6 0.7898720965906705 1102 7 0.7505736141914809 1102 8 0.6738975186575544 1102 9 0.7685696835533165 1102 10 0.49857062792064255 1102 11 0.7125116871454864 1102 12 0.736421266650062 1102 13 0.7795293474831837 1102 14 0.8037696331147117 1102 15 0.7247039906058106 1102 16 0.8531740449492702 1102 17 0.8998242377906109 1102 18 0.7901234795632632 1102 19 0.8648390688823682 1102 20 0.9051113492724667 1102 21 0.9910791688947853 1102 22 0.9379630991626655 1102 23 0.9624439223410093 1102 24 0.8937136388702385 1102 25 0.8907539443764918 1103 1 1.1389066586995291 1103 2 4.084271906724709 1103 3 2.285236376297341 1103 4 1.3802012036047628 1103 5 0.8563932953236417 1103 6 0.758577036510566 1103 7 0.6280190970575248 1103 8 0.4991133620042376 1103 9 0.5775149439004466 1103 10 0.49250031194377897 1103 11 0.5792317583515398 1103 12 0.6368396022085818 1103 13 0.6322307271004561 1103 14 0.7134358547435511 1103 15 0.7911748406280594 1103 16 0.6051099595020091 1103 17 0.8104784433980896 1103 18 0.663821743915733 1103 19 0.6492805464079581 1103 20 0.6496685366333921 1103 21 0.6592767656905139 1103 22 0.6550614525227019 1103 23 0.846039827992989 1103 24 0.8906428778555551 1103 25 0.7750797120667485 1104 1 1.1230414076132078 1104 2 3.8571115788117964 1104 3 2.1021220995424237 1104 4 1.339430697848364 1104 5 0.85488005785556 1104 6 0.6220443124143387 1104 7 0.5210451240252283 1104 8 0.4431513497299022 1104 9 0.5170526404466074 1104 10 0.5816084067339062 1104 11 0.4828029629387345 1104 12 0.5441388003450385 1104 13 0.5329797303706272 1104 14 0.5428593193316402 1104 15 0.512877120787536 1104 16 0.55725348859756 1104 17 0.5326233163990324 1104 18 0.3947181288102328 1104 19 0.49746582616426593 1104 20 0.4697160212904663 1104 21 0.5371399561219121 1104 22 0.5715789456911793 1104 23 0.7452487156872962 1104 24 0.69373386025039 1104 25 0.5360876411085442 1105 1 1.5716635509301078 1105 2 4.54179070371719 1105 3 2.7471286068737797 1105 4 1.7375069680157864 1105 5 1.287746678782291 1105 6 1.0031635026759744 1105 7 0.8269789968069006 1105 8 0.746306344460379 1105 9 0.8542066732237572 1105 10 0.8172622167056858 1105 11 0.8886553172487837 1105 12 0.7695338377975602 1105 13 0.8166542859977 1105 14 0.9182854788036963 1105 15 0.8611121839864566 1105 16 0.8077415384493776 1105 17 0.9729972403905691 1105 18 0.8079946762465582 1105 19 0.8664359348791351 1105 20 0.9487537496659684 1105 21 0.9889008258408936 1105 22 1.019622856031475 1105 23 0.9074934620782003 1105 24 1.0712819755999163 1105 25 1.1375233210893043 1106 1 0.40562225835518717 1106 2 2.589763275489151 1106 3 1.3291029885940198 1106 4 0.4606126031788662 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-0.6775456752984681 2315 11 -0.8086721134775701 2315 12 -0.720691921304244 2315 13 -0.7868257927037305 2315 14 -0.8977879992840414 2315 15 -1.2038526033713453 2315 16 -0.9110004098793922 2315 17 -0.9977873925312934 2315 18 -0.8312680053796271 2315 19 -0.9858359051570886 2315 20 -0.9057920775188109 2315 21 -1.0468232194748879 2315 22 -1.3161386661540533 2315 23 -1.066631882435729 2315 24 -0.8515615428535384 2315 25 -0.8676053494968201 2316 1 -0.5813776341220773 2316 2 -0.20186881822198544 2316 3 0.007382787100468136 2316 4 -0.29185281203134394 2316 5 -0.7925192846337126 2316 6 -0.744535969886023 2316 7 -0.8768280955508629 2316 8 -0.9711496088557467 2316 9 -0.980267572091023 2316 10 -0.90661838030924 2316 11 -1.060228085287413 2316 12 -0.9500402840742836 2316 13 -1.1351608862613105 2316 14 -1.2115961281988845 2316 15 -0.992734185669029 2316 16 -1.2667450511589777 2316 17 -1.3924639873055327 2316 18 -1.1444938211162494 2316 19 -1.0345431920426478 2316 20 -0.9961878954917012 2316 21 -1.173089972049354 2316 22 -1.0401908573564782 2316 23 -1.157257968737646 2316 24 -1.2539200219312292 2316 25 -1.0576708209822954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21542.0 3 0.0 4 164.0 5 821.0 6 992.0 7 872.0 8 804.0 9 748.0 10 708.0 11 733.0 12 639.0 13 674.0 14 681.0 15 713.0 16 735.0 17 808.0 18 914.0 19 1143.0 20 1473.0 21 1776.0 22 2172.0 23 2528.0 24 2868.0 25 3329.0 26 3937.0 27 4529.0 28 5323.0 29 6447.0 30 8545.0 31 11503.0 32 16128.0 33 23849.0 34 37252.0 35 61189.0 36 114208.0 37 172932.0 38 493065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.828198628449734 20.23960410988295 14.259732364930905 25.672464896736408 2 15.280009016023932 21.8884471947871 41.63743289209458 21.194110897094383 3 17.00352820578022 26.001843566984256 31.281139991894662 25.713488235340858 4 11.169771063944758 18.42702812234292 40.43709224986689 29.966108563845427 5 11.77051961571164 38.70934418283099 37.266276233084085 12.25385996837329 6 28.49463220044023 39.16616339407039 20.003496420142557 12.33570798534682 7 25.434370604642293 32.486312309782825 24.67419721398886 17.405119871586024 8 22.538897674085963 39.00614257447772 22.470757213353146 15.984202538083167 9 25.151975080060073 15.743823653282266 22.486948022536016 36.61725324412164 10 14.181062911723338 29.720763173160208 36.079877307438636 20.018296607677822 11 31.245679139880643 22.822087839609672 27.81630682676033 18.115926193749353 12 22.363778676604976 25.08671519274016 34.09943342100872 18.450072709646147 13 27.423257551075547 23.470018197277557 27.292737776435715 21.813986475211173 14 18.847889830979874 24.11238606835501 29.513759207901906 27.525964892763206 15 20.745989049847825 34.02612779415621 24.509209888511876 20.718673267484085 16 19.34583171094141 28.97151609545227 31.352657676628816 20.329994516977504 17 19.274910006913377 30.22615481194822 30.356773916705738 20.142161264432666 18 19.018893637986334 28.150338567715032 33.61460395474109 19.21616383955754 19 21.98981066592235 27.438293341173832 29.335408680899416 21.2364873120044 20 21.836068569975506 28.66088297054447 30.473370430042372 19.02967802943765 21 20.644352528419137 27.7210255921233 29.066588789688453 22.56803308976911 22 21.201640171486922 30.110536750887036 29.306344141989264 19.38147893563678 23 19.625919432621295 29.650503856526296 30.25294640595589 20.470630304896524 24 21.405484225352673 27.774047372613836 31.118399748892962 19.702068653140525 25 20.722728807835193 28.59407592824364 30.50807557065379 20.175119693267376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 263.0 1 263.0 2 343.0 3 423.0 4 423.0 5 423.0 6 954.5 7 1486.0 8 1486.0 9 1486.0 10 2027.5 11 2569.0 12 2569.0 13 2569.0 14 4458.0 15 6347.0 16 6347.0 17 6347.0 18 11329.0 19 16311.0 20 16311.0 21 16311.0 22 26232.0 23 36153.0 24 36153.0 25 36153.0 26 52379.0 27 68605.0 28 68605.0 29 68605.0 30 87815.0 31 107025.0 32 107025.0 33 107025.0 34 116131.0 35 125237.0 36 125237.0 37 125237.0 38 128055.0 39 130873.0 40 130873.0 41 130873.0 42 131134.5 43 131396.0 44 131396.0 45 131396.0 46 127549.0 47 123702.0 48 123702.0 49 123702.0 50 112607.5 51 101513.0 52 101513.0 53 101513.0 54 84701.0 55 67889.0 56 67889.0 57 67889.0 58 55544.0 59 43199.0 60 43199.0 61 43199.0 62 33796.5 63 24394.0 64 24394.0 65 24394.0 66 18085.0 67 11776.0 68 11776.0 69 11776.0 70 8278.5 71 4781.0 72 4781.0 73 4781.0 74 3361.0 75 1941.0 76 1941.0 77 1941.0 78 1265.0 79 589.0 80 589.0 81 589.0 82 379.0 83 169.0 84 169.0 85 169.0 86 109.0 87 49.0 88 49.0 89 49.0 90 40.5 91 32.0 92 32.0 93 32.0 94 23.5 95 15.0 96 15.0 97 15.0 98 11.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.0498319334408746 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.979903530589703E-4 19 4.966505884316172E-4 20 5.959807061179406E-4 21 0.002781243295217056 22 0.0019866023537264688 23 8.939710591769109E-4 24 1.9866023537264685E-4 25 3.973204707452937E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1006744.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.39553915521107 #Duplication Level Percentage of deduplicated Percentage of total 1 90.21644601325617 69.82350479862954 2 7.179433256774464 11.113122154738251 3 1.313047076481552 3.0487195926139017 4 0.44508231540654686 1.377895430773576 5 0.21796244484453314 0.8434660467165299 6 0.14379356353359643 0.6677388226039076 7 0.08209250957952949 0.444751582765841 8 0.06059572598424066 0.37518711064413907 9 0.04805705587988589 0.33474615750322867 >10 0.24175755686304798 3.3777899903730813 >50 0.027563431005014308 1.4857932401858727 >100 0.019101347500314246 2.954605832114474 >500 0.0031185863945033102 1.4621670898786585 >1k 0.001949116496564569 2.690512150459024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTAAAGTGTGTATTTCTCATTT 3074 0.30534078176775825 No Hit GTCCTACAGTGGACATTTCTAAATT 3007 0.29868566388277457 No Hit CTGTAGGACGTGGAATATGGCAAGA 2975 0.2955071001168122 No Hit CTTTAGGACGTGAAATATGGCGAGG 2900 0.28805734129033794 No Hit GTATCAACGCAGAGTACTTTTTTTT 2393 0.237696971623372 No Hit TATCAACGCAGAGTACTTTTTTTTT 1528 0.15177641982470222 No Hit GGTATCAACGCAGAGTACTTTTTTT 1494 0.14839919582336722 No Hit CTGAAGGACCTGGAATATGGCGAGA 1439 0.1429360393506194 No Hit GTCCTACAGTGTGCATTTCTCATTT 1276 0.12674523016774872 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1237 0.12287135557798208 No Hit ATTTAGAAATGTCCACTGTAGGACG 1186 0.11780551957597958 No Hit GAATATGGCAAGAAAACTGAAAATC 1081 0.10737585721891563 No Hit CTGTAGGACCTGGAATATGGCGAGA 1073 0.10658121627742505 No Hit GGAATATGGCGAGAAAACTGAAAAT 1072 0.1064818861597387 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1018 0.10111805980467727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 9.933011768632344E-5 0.0 0.0 0.0 3 0.0 9.933011768632344E-5 0.0 0.0 0.0 4 0.0 9.933011768632344E-5 0.0 0.0 0.0 5 0.0 9.933011768632344E-5 0.0 0.0 0.0 6 0.0 9.933011768632344E-5 0.0 0.0 0.0 7 0.0 9.933011768632344E-5 0.0 0.0 0.0 8 0.0 9.933011768632344E-5 0.0 0.0 0.0 9 0.0 9.933011768632344E-5 0.0 0.0 0.0 10 0.0 9.933011768632344E-5 0.0 0.0 0.0 11 0.0 9.933011768632344E-5 0.0 0.0 0.0 12 0.0 9.933011768632344E-5 0.0 0.0 0.0 13 0.0 9.933011768632344E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 35 0.0018022226 16.743881 1 GGCGAAA 40 2.8275783E-4 16.57228 19 TGGCGAA 50 8.948195E-5 15.151801 18 CGGTAGG 40 0.0043894737 14.650896 1 TCCAACG 150 0.0 13.257826 18 TAGGACC 395 0.0 13.185247 4 CCAACGT 65 5.6135486E-5 13.112135 19 GCTTAGA 70 8.4116895E-5 12.557911 2 CCAACGA 100 1.5012665E-7 12.310838 19 GTATTAG 105 1.8939863E-7 12.092803 1 GGCCTAC 105 1.8939863E-7 12.092803 1 AAATGTC 435 0.0 11.972812 7 TGCCGGT 115 7.384551E-8 11.527971 10 GACGTGA 970 0.0 11.519484 7 AGGACGT 1920 0.0 11.442198 5 GGACGTG 1905 0.0 11.432878 6 ACTGATC 75 2.1354483E-4 11.363287 8 GGTATCA 575 0.0 11.211122 1 ACGTGAA 1090 0.0 11.120034 8 ACCTTTT 495 0.0 11.095465 15 >>END_MODULE