FastQCFastQC Report
Thu 26 May 2016
SRR1511964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006744
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34120.33891436154573557No Hit
CTGTAGGACGTGGAATATGGCAAGA27930.2774290186979013No Hit
GTCCTAAAGTGTGTATTTCTCATTT27330.27146921163672194No Hit
CTTTAGGACGTGAAATATGGCGAGG26420.2624301709272665No Hit
TATCAACGCAGAGTACTTTTTTTTT25630.254583091630047No Hit
GTCCTACAGTGGACATTTCTAAATT24900.24733199303894537No Hit
GGTATCAACGCAGAGTACTTTTTTT22500.22349276479422775No Hit
CTGAAGGACCTGGAATATGGCGAGA15480.1537630221784287No Hit
TTTCTAAATTTTCCACCTTTTTCAG15120.15018713794172103No Hit
GAATATGGCAAGAAAACTGAAAATC12440.12356666640178636No Hit
ATTTAGAAATGTCCACTGTAGGACG12250.12167939416574622No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11580.11502427628076253No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10720.1064818861597387No Hit
GTCCTACAGTGTGCATTTCTCATTT10600.10528992474750284No Hit
CTGTAGGACCTGGAATATGGCGAGA10560.10489260427675755No Hit
GGAATATGGCGAGAAAACTGAAAAT10270.10201203086385416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCA400.005291417214.2443019
GAACTGT1102.741217E-912.9545136
TAGGACT1251.4024408E-912.162994
GTCATAA550.002972602412.1415521
ATCCCGT550.003076357512.08607410
GTGTAGA1052.5725603E-711.811171
TAGGACC3550.011.2422014
GGCGAGG8300.011.21293819
TATACTG1451.209628E-911.140675
CCACCTT3850.011.09945513
ACATACG600.005890737311.07898
GGTATCA8700.010.9652771
CACCTTT3900.010.95715414
AATCCCG1408.376446E-910.8533419
TCCAACG803.7821417E-410.68322618
TCCACCT3850.010.60614612
GACGTGA9250.010.5741477
AGGACCT9650.010.240865
GGACGTG16900.010.2307426
ACTGTTC3550.010.16494758
Produced by FastQC (version 0.11.5)