##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511964_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1006744 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.959041225972044 31.0 30.0 33.0 25.0 34.0 2 29.184280214235198 31.0 30.0 34.0 25.0 34.0 3 29.190678067115375 31.0 29.0 34.0 25.0 34.0 4 32.71840010966045 35.0 32.0 37.0 26.0 37.0 5 32.23121866134787 35.0 32.0 37.0 25.0 37.0 6 32.21705815977051 35.0 32.0 37.0 23.0 37.0 7 31.97959759382723 35.0 32.0 37.0 22.0 37.0 8 31.827248039223477 35.0 32.0 37.0 19.0 37.0 9 32.988316791557736 37.0 32.0 39.0 19.0 39.0 10 32.63633853293389 37.0 32.0 39.0 17.0 39.0 11 32.70658081895695 37.0 32.0 39.0 17.0 39.0 12 32.437404146436435 37.0 32.0 39.0 17.0 39.0 13 32.43294025094761 37.0 32.0 39.0 17.0 39.0 14 32.99626618087617 38.0 32.0 40.0 16.0 41.0 15 32.91087307200242 38.0 32.0 40.0 11.0 41.0 16 32.739779924191254 37.0 32.0 40.0 11.0 41.0 17 32.624548047964524 37.0 32.0 40.0 10.0 41.0 18 32.48065545958059 37.0 32.0 40.0 10.0 41.0 19 32.52116128827189 37.0 32.0 40.0 10.0 41.0 20 32.34370604642292 37.0 31.0 40.0 8.0 41.0 21 32.14534578800569 37.0 31.0 40.0 2.0 41.0 22 31.956741733747606 37.0 31.0 40.0 2.0 41.0 23 31.979259871427097 37.0 31.0 40.0 2.0 41.0 24 31.75922975453541 37.0 31.0 40.0 2.0 41.0 25 31.16666600446588 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46828690045297705 1101 2 0.2756307726504943 1101 3 0.39260976616392895 1101 4 0.23794041032707014 1101 5 0.4861679848198719 1101 6 0.6787478224740582 1101 7 0.308590245195024 1101 8 0.27903621121347655 1101 9 0.020300855603444745 1101 10 -0.18955136717238474 1101 11 0.008734428205499967 1101 12 0.30073330990886404 1101 13 -0.008677678166471026 1101 14 -0.08751189701693107 1101 15 -0.26889037456575693 1101 16 0.4398574930901944 1101 17 0.5268532349553894 1101 18 0.4233110438630021 1101 19 0.30536337788341683 1101 20 0.03315231048933498 1101 21 0.13722128907541276 1101 22 0.022610227372808822 1101 23 -0.48339035619787296 1101 24 -0.3470778128275356 1101 25 -0.14965713806828518 1102 1 0.23932944149379765 1102 2 0.4232888851807495 1102 3 0.05762694393344603 1102 4 0.4146312195405173 1102 5 0.5686502858251572 1102 6 0.45156796805451904 1102 7 0.5223188636309892 1102 8 0.5518889900236381 1102 9 0.5659538374648108 1102 10 0.5318877582570636 1102 11 0.512974935108339 1102 12 0.5269087860367492 1102 13 0.602394957963952 1102 14 0.5925374154190521 1102 15 0.5381076402461034 1102 16 0.5971360630295521 1102 17 0.5984333178075403 1102 18 0.6015265500859783 1102 19 0.641653342258806 1102 20 0.9631331837908235 1102 21 0.8910860605974804 1102 22 0.8652011245146838 1102 23 0.8507240595313483 1102 24 1.1394174689513399 1102 25 1.2154298385536286 1103 1 0.4375492901809359 1103 2 0.4253716622167971 1103 3 0.4841066947122634 1103 4 0.6108127949744286 1103 5 0.4223307486644927 1103 6 0.4467437579966642 1103 7 0.650961498254933 1103 8 0.24858680933827415 1103 9 0.529754460517772 1103 10 0.29586639644130486 1103 11 0.7788494344407795 1103 12 0.7210787904871268 1103 13 0.6228755988184247 1103 14 0.5127421328200299 1103 15 0.46354600250689515 1103 16 0.4295882214630211 1103 17 0.8148196106424308 1103 18 0.3769159581856698 1103 19 0.7109012283291207 1103 20 0.5200268197498801 1103 21 0.7724122733255641 1103 22 1.0133275152578989 1103 23 0.965169987435214 1103 24 0.9595509802997988 1103 25 1.1477840886648885 1104 1 0.10898682097876744 1104 2 -0.1013908697009569 1104 3 0.20220014093364824 1104 4 0.3911568347540282 1104 5 0.05442410529219188 1104 6 0.46899703936565373 1104 7 0.35912617146193426 1104 8 0.35305215897766473 1104 9 0.3579074865447396 1104 10 0.3410243053245239 1104 11 0.48800680576184163 1104 12 0.4127107395979053 1104 13 0.36079254546538664 1104 14 0.7617991592053741 1104 15 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-0.597928611622109 2315 4 -0.5936761532528365 2315 5 -0.34806830192146876 2315 6 -0.4069990101386267 2315 7 -0.39990335859123505 2315 8 -0.36887944195974853 2315 9 -0.37817908049988347 2315 10 -0.0735120340586164 2315 11 -0.31433555294981375 2315 12 -0.08575995747311538 2315 13 -0.19023224868194433 2315 14 -0.37942520140337876 2315 15 -0.1975102206054089 2315 16 -0.4656884437201896 2315 17 -0.5879633592433464 2315 18 -0.6360643118039491 2315 19 -0.4512853908668433 2315 20 -0.5065449055134366 2315 21 -0.8263594222685633 2315 22 -0.6777895296909264 2315 23 -0.5412800941550522 2315 24 -0.5784433825543758 2315 25 -0.7510291437932004 2316 1 -1.2198778597835265 2316 2 -1.5346709817064443 2316 3 -1.3691271361335104 2316 4 -1.6720017039594737 2316 5 -1.6441369130270473 2316 6 -1.5672292341852838 2316 7 -1.5368565543392414 2316 8 -1.5145119824172326 2316 9 -2.043280365352878 2316 10 -2.0417028999339593 2316 11 -2.3276324418918435 2316 12 -2.3690420339620566 2316 13 -2.1573802647210165 2316 14 -2.9554170711600705 2316 15 -2.02747034433035 2316 16 -2.0637451118703147 2316 17 -2.074533623188419 2316 18 -2.410575157663658 2316 19 -1.9725387148123126 2316 20 -2.124422362908259 2316 21 -2.0112693187142376 2316 22 -1.8293448372652037 2316 23 -1.6794355562748038 2316 24 -2.025694222258835 2316 25 -1.7355137080657421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40385.0 3 0.0 4 648.0 5 4749.0 6 6020.0 7 4984.0 8 4085.0 9 3717.0 10 3488.0 11 3315.0 12 3064.0 13 2972.0 14 2811.0 15 2942.0 16 3032.0 17 3119.0 18 3308.0 19 3766.0 20 4522.0 21 5776.0 22 7310.0 23 8951.0 24 10575.0 25 12494.0 26 14229.0 27 16264.0 28 18946.0 29 22907.0 30 28332.0 31 36404.0 32 45952.0 33 59505.0 34 77054.0 35 99893.0 36 133266.0 37 170759.0 38 137200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.318956618524865 24.150287783632187 16.53726127322939 20.993494324613554 2 15.698727779852673 24.903153135255835 40.605754789698274 18.792364295193217 3 19.456286801808602 28.617006905429783 31.80212645915943 20.124579833602187 4 14.590700317061737 21.71555032858403 38.53343054440851 25.160318809945725 5 12.395889433634544 40.4019547249454 36.391251035888956 10.8109048055311 6 29.130604141286266 40.83404050662093 18.77699099919114 11.258364352901664 7 25.943834778255447 33.680061664137064 24.353062943508977 16.02304061409852 8 23.736322242794593 39.97381658097788 21.638768147612502 14.65109302861502 9 26.409295709733556 15.81633463919328 22.22153794807816 35.552831702995 10 14.943918215554303 31.54903331929468 35.17418529437474 18.332863170776285 11 32.74745118918017 23.318937088276662 27.0728208958782 16.86079082666497 12 22.989459087911126 26.432240966919096 33.506432618421364 17.071867326748407 13 28.744149456068275 23.93547912875567 27.05931199987286 20.261059415303194 14 19.910920750458907 24.940103939035147 29.305761941466745 25.843213369039198 15 21.715153008113283 34.93191913733779 24.039577092090937 19.313350762457983 16 20.323736719563264 30.20112362229127 30.308698139745555 19.16644151839991 17 20.298606199788622 31.39040312134962 29.406383350682997 18.904607328178763 18 20.012237470498956 28.96317236556662 32.98177093680221 18.04281922713222 19 23.077664232416584 28.042282844496714 29.267122525686766 19.612930397399936 20 22.90197200278522 29.43541698328173 29.844955465297502 17.81765554863555 21 21.895933822302393 28.316433969310967 28.826792113983295 20.960840094403345 22 22.404305364620996 30.44527705156425 29.07958726349499 18.070830320319764 23 20.89150767225829 30.252079972664355 29.82863568096755 19.027776674109802 24 22.240940877304514 28.808506834075015 30.483252542911632 18.467299745708836 25 21.787245540628067 29.53238682195493 29.9177266243081 18.762641013108905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 288.0 1 288.0 2 384.0 3 480.0 4 480.0 5 480.0 6 1075.0 7 1670.0 8 1670.0 9 1670.0 10 2456.0 11 3242.0 12 3242.0 13 3242.0 14 5308.0 15 7374.0 16 7374.0 17 7374.0 18 13244.5 19 19115.0 20 19115.0 21 19115.0 22 30380.0 23 41645.0 24 41645.0 25 41645.0 26 58769.5 27 75894.0 28 75894.0 29 75894.0 30 94741.0 31 113588.0 32 113588.0 33 113588.0 34 121425.0 35 129262.0 36 129262.0 37 129262.0 38 130283.5 39 131305.0 40 131305.0 41 131305.0 42 130263.0 43 129221.0 44 129221.0 45 129221.0 46 123667.5 47 118114.0 48 118114.0 49 118114.0 50 106706.0 51 95298.0 52 95298.0 53 95298.0 54 78735.5 55 62173.0 56 62173.0 57 62173.0 58 50450.0 59 38727.0 60 38727.0 61 38727.0 62 30131.0 63 21535.0 64 21535.0 65 21535.0 66 16083.5 67 10632.0 68 10632.0 69 10632.0 70 7637.0 71 4642.0 72 4642.0 73 4642.0 74 3174.0 75 1706.0 76 1706.0 77 1706.0 78 1157.0 79 608.0 80 608.0 81 608.0 82 387.0 83 166.0 84 166.0 85 166.0 86 106.5 87 47.0 88 47.0 89 47.0 90 29.0 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40536621027788594 2 0.0 3 0.0 4 0.0 5 0.035957502602449086 6 0.03873874589766614 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 9.933011768632343E-5 21 0.0 22 0.0 23 0.0 24 0.0023839228244717623 25 0.009933011768632344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1006744.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.39330956688765 #Duplication Level Percentage of deduplicated Percentage of total 1 91.14908175651915 70.54329101119838 2 6.3381911939166296 9.81067186329822 3 1.2357975618230277 2.8692738979252366 4 0.44831348085943856 1.3878585602865394 5 0.20820952909703572 0.8057012270091393 6 0.12742199297938672 0.5916965848970076 7 0.08974380442331818 0.486189902621086 8 0.05889188166355437 0.36462701028511824 9 0.047119973071960475 0.32820935964674824 >10 0.24624227889937828 3.5172114089066606 >50 0.02313636017142331 1.259022652105295 >100 0.02286781592093779 3.581157424935686 >500 0.0029383211553714967 1.5101762326243475 >1k 0.002044049499388867 2.9449128642605857 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3412 0.33891436154573557 No Hit CTGTAGGACGTGGAATATGGCAAGA 2793 0.2774290186979013 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2733 0.27146921163672194 No Hit CTTTAGGACGTGAAATATGGCGAGG 2642 0.2624301709272665 No Hit TATCAACGCAGAGTACTTTTTTTTT 2563 0.254583091630047 No Hit GTCCTACAGTGGACATTTCTAAATT 2490 0.24733199303894537 No Hit GGTATCAACGCAGAGTACTTTTTTT 2250 0.22349276479422775 No Hit CTGAAGGACCTGGAATATGGCGAGA 1548 0.1537630221784287 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1512 0.15018713794172103 No Hit GAATATGGCAAGAAAACTGAAAATC 1244 0.12356666640178636 No Hit ATTTAGAAATGTCCACTGTAGGACG 1225 0.12167939416574622 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1158 0.11502427628076253 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1072 0.1064818861597387 No Hit GTCCTACAGTGTGCATTTCTCATTT 1060 0.10528992474750284 No Hit CTGTAGGACCTGGAATATGGCGAGA 1056 0.10489260427675755 No Hit GGAATATGGCGAGAAAACTGAAAAT 1027 0.10201203086385416 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 9.933011768632344E-5 0.0 8 0.0 0.0 0.0 9.933011768632344E-5 0.0 9 0.0 0.0 0.0 9.933011768632344E-5 0.0 10 0.0 0.0 0.0 9.933011768632344E-5 0.0 11 0.0 0.0 0.0 9.933011768632344E-5 0.0 12 0.0 0.0 0.0 9.933011768632344E-5 0.0 13 0.0 0.0 0.0 9.933011768632344E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGCA 40 0.0052914172 14.244301 9 GAACTGT 110 2.741217E-9 12.954513 6 TAGGACT 125 1.4024408E-9 12.16299 4 GTCATAA 55 0.0029726024 12.141552 1 ATCCCGT 55 0.0030763575 12.086074 10 GTGTAGA 105 2.5725603E-7 11.81117 1 TAGGACC 355 0.0 11.242201 4 GGCGAGG 830 0.0 11.212938 19 TATACTG 145 1.209628E-9 11.14067 5 CCACCTT 385 0.0 11.099455 13 ACATACG 60 0.0058907373 11.0789 8 GGTATCA 870 0.0 10.965277 1 CACCTTT 390 0.0 10.957154 14 AATCCCG 140 8.376446E-9 10.85334 19 TCCAACG 80 3.7821417E-4 10.683226 18 TCCACCT 385 0.0 10.606146 12 GACGTGA 925 0.0 10.574147 7 AGGACCT 965 0.0 10.24086 5 GGACGTG 1690 0.0 10.230742 6 ACTGTTC 355 0.0 10.1649475 8 >>END_MODULE