FastQCFastQC Report
Thu 26 May 2016
SRR1511963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511928
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT40890.2704493864787212No Hit
CTGTAGGACGTGGAATATGGCAAGA38680.25583228831002536No Hit
GTCCTAAAGTGTGTATTTCTCATTT33910.22428316692329262No Hit
CTTTAGGACGTGAAATATGGCGAGG30070.19888513209623737No Hit
GTATCAACGCAGAGTACTTTTTTTT26600.175934303749914No Hit
GTCCTACAGTGTGCATTTCTCATTT19260.12738701842944902No Hit
CTGAAGGACCTGGAATATGGCGAGA17430.1152832674571805No Hit
GGTATCAACGCAGAGTACTTTTTTT15510.10258425004365287No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTC402.7638793E-416.62277213
TAGGACC5800.014.0843194
TCCAACG1950.013.15251818
CCAACGA1051.995977E-812.66538819
GGCGAGG8700.012.33783519
CCAACGT1103.8184226E-812.08968819
GGTATCA6950.011.768221
CGGTAGG657.9501385E-411.7050641
CTATACC1552.5465852E-1111.6436014
TATGTCG1157.0816895E-811.5640516
AATCCCG2250.011.39884919
TGTCGAG1104.9737173E-711.22613918
TGCACCG600.005881399411.0818475
GGACTGT2250.010.9763076
TGGCGAG19550.010.932418
ACTGTTC5450.010.8058918
TGTAGGA20650.010.5462522
TTAGGAC12850.010.4966473
GTCCTAA10350.010.3832951
AGGACGT21700.010.3741865
Produced by FastQC (version 0.11.5)