FastQCFastQC Report
Thu 26 May 2016
SRR1511963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511928
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT45720.30239535215962665No Hit
CTGTAGGACGTGGAATATGGCAAGA41160.2722351858024985No Hit
GTCCTAAAGTGTGTATTTCTCATTT34920.23096337920853377No Hit
CTTTAGGACGTGAAATATGGCGAGG33680.22276193046229714No Hit
GTATCAACGCAGAGTACTTTTTTTT26080.17249498653375028No Hit
GTCCTACAGTGTGCATTTCTCATTT21840.1444513230788768No Hit
CTGAAGGACCTGGAATATGGCGAGA18780.12421226407606711No Hit
CTGTAGGACCTGGAATATGGCGAGA16060.10622198940690299No Hit
TATCAACGCAGAGTACTTTTTTTTT15250.10086459143557101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT759.672385E-713.9333119
TAGGACC6550.013.2032564
TCCAACG1950.012.17946518
GGACCGT550.00307222212.0888886
GGTATCA6700.011.3956781
GGCGAGG9300.011.3386919
CTAGGAC2350.011.323253
TATGTCG855.3494674E-511.17090716
GTGTTAC1303.0504452E-811.0121691
GTGGTAT3050.010.9519931
GTCCTAC20650.010.9072911
AAAGGCG700.001486860110.8610775
AGGACCT13150.010.6236215
AGGACGT19550.010.5485785
TGTAGGA20700.010.513472
TGTCGAG1002.398299E-510.44998218
GGACGTG19100.010.4432816
GTCCTAA10700.010.4358321
GGACCTG13050.010.4082216
TCCTACA22100.010.4064852
Produced by FastQC (version 0.11.5)